Protein Alignment nAChRalpha3 and nAChRbeta1

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_309158.3
• Gene: nAChRbeta1 / 1270461
• VectorBaseID: AGAMI1_011382
• Length: 519
• Species: Anopheles gambiae
• Alignment Length: 766
• Identity: 247/766 - (32%)
• Similarity: 344/766 - (44%)
• Gaps: 272/766 - (35%)

  Fly    11 ILVLS-------FISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLK 68
            :|:||       ::...:.:.|.:||..||...||||:|||.|:|..:.||..|...|||:||.|
Mosquito     5 VLILSVLLALQQYLGMGSCSEDEERLVRDLFRGYNKLIRPVQNMTQKVDVRFGLAFVQLINVNEK 69

  Fly    69 NQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLN 133
            ||||.:|:|:...|.||:|:|:..:|||:.:|.:|.|.:|:|||||:||||||:||...:...:.
Mosquito    70 NQIMKSNVWLRLVWSDYQLQWDEADYGGIGVLRLPPDKVWKPDIVLFNNADGNYEVRYKSNVLIY 134

  Fly   134 YTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDL 198
            ..|.|.|.|||||:|||.|||.|||||:|||:|||||||::|.||.|...:..|       .|||
Mosquito   135 PNGEVLWVPPAIYQSSCTIDVTYFPFDQQTCIMKFGSWTFNGDQVSLALYNNKN-------FVDL 192

  Fly   199 SEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVF 263
            |:::.|..|||:||||..|  .|. .:....||||.|.:||||||||||||:|.:.||||.:|||
Mosquito   193 SDYWKSGTWDIIEVPAYLN--VYE-GNPTETDITFYIIIRRKTLFYTVNLILPTVLISFLCVLVF 254

  Fly   264 YLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNI 328
            |||:::||||:|.|||||||.||.||:::|:||||||:||:.|::|||.|::|.||.|||:::|.
Mosquito   255 YLPAEAGEKVTLGISILLSLVVFLLLVSKILPPTSLVLPLIAKYLLFTFIMNTVSILVTVIIINW 319

  Fly   329 HFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPP 393
            :||.|:||.|..|:|:||::.||..|:|:||        :.:|...:....|:    .:||.|
Mosquito   320 NFRGPRTHRMPMWIRSVFLHYLPAMLLMKRP--------RKTRLRWMMEMPGM----SVPPQP-- 370

  Fly   394 VALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTS 458
                                  |...:|           |..:.|..|:|:|      .|.|...
Mosquito   371 ----------------------HTHPSY-----------GSPAEIPKHISAL------GAKQSKM 396

  Fly   459 TTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHH 523
            ....|.|              |.||
Mosquito   397 EVMELSD--------------LHHP---------------------------------------- 407

  Fly   524 LYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQI 588
                  :|..|            ....:.|||:.                    ||||
Mosquito   408 ------NCKMN------------RKLNSGDLGIG--------------------ADSC------- 427

  Fly   589 HQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAA 653
                                                     :|.:.|:.:...:
Mosquito   428 -----------------------------------------RRESESSDSILLS----------- 440

  Fly   654 AAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIA 718
                                                               ||..||.:.|.:||
Mosquito   441 ---------------------------------------------------PEASKATEAVEFIA 454

  Fly   719 DQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYD 769
            :..|.|:...:.:|||||||||:|||.|:||.|....||.||::.||.:::
Mosquito   455 EHLRNEDLYIQTREDWKYVAMVIDRLQLYIFFIVTTAGTVGILMDAPHIFE 505

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	111/217 (51%)
		Cys-loop	150..164	CDD:349832	10/13 (77%)
		LGIC_TM_nAChR	242..>331	CDD:349866	56/88 (64%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	13/20 (65%)
		TM3 helix	306..328	CDD:349866	11/21 (52%)
		Neur_chan_memb	<683..760	CDD:460753	27/76 (36%)
nAChRbeta1	XP_309158.3	LIC	6..498	CDD:455710	245/756 (32%)
		LGIC_ECD_nAChR_proto_beta-like	26..232	CDD:349833	109/215 (51%)
		Cys-loop	151..165	CDD:349833	10/13 (77%)