Protein Alignment nAChRalpha3 and Chrnb2

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_033732.2
• Gene: Chrnb2 / 11444
• MGIID: 87891
• Length: 501
• Species: Mus musculus
• Alignment Length: 762
• Identity: 240/762 - (31%)
• Similarity: 331/762 - (43%)
• Gaps: 298/762 - (39%)

  Fly    10 MILVLSF----ISSSTANPDA-KRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNL 67
            |.|:.||    :.|.....|. :||.:.||  |.||||:||..|.::.:||::.:.|:|||.|:.
Mouse     8 MALLFSFGLLWLCSGVLGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHE 72

  Fly    68 KNQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATL 132
            :.||||||:|:.|.|.||:|.|:|:::..::.:.:||.|||.||:||||||||.:||:..:.|.:
Mouse    73 REQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVV 137

  Fly   133 NYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGV- 196
            :|.|.:.|.|||||||:|:|:|::||||:|.|.|||.|||||..::||          |::..|
Mouse   138 SYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL----------VLKSDVA 192

  Fly   197 DLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTIL 261
            .|.:|..|.||||:.:|..|||   ...|..|:|||::..:|||.||||:||||||:.|:.|.||
Mouse   193 SLDDFTPSGEWDIIALPGRRNE---NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAIL 254

  Fly   262 VFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVL 326
            |||||||.|||::|.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.||
Mouse   255 VFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVL 319

  Fly   327 NIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLP 391
            |:|.|||.||||||||:.||:.:||..|.:::|.:..:.     :||.:|
Mouse   320 NVHHRSPTTHTMAPWVKVVFLEKLPTLLFLQQPRHRCAR-----QRLRLR--------------- 364

  Fly   392 PPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQ 456
                                                                             
Mouse   365 ----------------------------------------------------------------- 364

  Fly   457 TSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGG 521
                        ..|.::.|
Mouse   365 ------------RRQREREG--------------------------------------------- 372

  Fly   522 HHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTI 586
                                                                         :||:
Mouse   373 -------------------------------------------------------------AGTL 376

  Fly   587 QIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTS 651
            ...:|..|.                                    |.|.....|:.....|
Mouse   377 FFREGPAAD------------------------------------PCTCFVNPASMQGLAG---- 401

  Fly   652 AAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTY 716
             |..|..|||..|                   |..||..|.              |.:|:|||.:
Mouse   402 -AFQAEPAAAGLG-------------------RSMGPCSCG--------------LREAVDGVRF 432

  Fly   717 IADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            |||..|.|::...|:|||||||||:||||||||....|.||.|:.||
Mouse   433 IADHMRSEDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQ 479

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	102/221 (46%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	59/88 (67%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	35/76 (46%)
Chrnb2	NP_033732.2	LIC	29..478	CDD:455710	232/738 (31%)
		LGIC_ECD_nAChR_B2	31..234	CDD:349826	100/215 (47%)
		Cys-loop	155..169	CDD:349826	8/13 (62%)