Protein Alignment nAChRalpha3 and Chrna6

DIOPT Version :9

• Sequence 1: NP_001285015.1
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_067344.2
• Gene: Chrna6 / 11440
• MGIID: 106213
• Length: 494
• Species: Mus musculus
• Alignment Length: 763
• Identity: 233/763 - (30%)
• Similarity: 333/763 - (43%)
• Gaps: 299/763 - (39%)

  Fly    14 LSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWV 78
            |:....||.....::|:..|.::||:.:|||.||:|.:||..:|.::||.:|:..||||.||||:
Mouse    22 LALFKGSTGCESEEQLFHRLFAHYNRFIRPVENVSDPVTVHFELAITQLANVDEVNQIMETNLWL 86

  Fly    79 EQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPP 143
            ...|.||:|:|:|.||.|:|.|.||:|:||:|||||||||.|:|:|...|||.|.|.|.:.|.||
Mouse    87 RHIWKDYRLRWDPTEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALLKYDGVITWTPP 151

  Fly   144 AIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWD 208
            ||:||||.:|:.:||||.|.|.:||||||||..::||.         ::...||:::|:.:.||:
Mouse   152 AIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLL---------IIGSKVDMNDFWENSEWE 207

  Fly   209 ILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKV 273
            |::....:::..|.||:|.|.|||::..:||..:|||:||||||:.|||||:||||||||.||||
Mouse   208 IVDASGYKHDIKYNCCEEIYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKV 272

  Fly   274 SLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTM 338
            :|.||:|||||||.|::.|.||.||||:||:|:::|||||..|.||.|||.|||||:|:|.||||
Mouse   273 TLCISVLLSLTVFLLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTM 337

  Fly   339 APWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSP 403
            ..||:|:|:...|..|:||:||
Mouse   338 PKWVKTIFLQAFPSILMMRKPL------------------------------------------- 359

  Fly   404 KTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQY 468
                                                                             
Mouse   360 ----------------------------------------------------------------- 359

  Fly   469 HNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSA 533
             ::.::.||  :..|.:.||
Mouse   360 -DKTKEAGG--VKDPKSHTK--------------------------------------------- 376

  Fly   534 NFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAAC 598
                                    .|..:|.|                       |:|
Mouse   377 ------------------------RPAKVKFT-----------------------HRG------- 387

  Fly   599 QSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAA 663
             .|:||       ::.||.|:..
Mouse   388 -ESKLL-------KECHHCQKSS------------------------------------------ 402

  Fly   664 GTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRW------HTCPELHKAMDGVTYIADQTR 722
                         ||.|..:|.         .....||      |: .::...::.|.:||:..:
Mouse   403 -------------DIAPGKRRS---------SQQPARWVAENSEHS-SDVEDVIESVQFIAENMK 444

  Fly   723 KEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDT 770
            ...|:..|::||||:|||:||:|||:|.|..|.||.|:.|| |.|.:|
Mouse   445 SHNETNEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVGLFLQ-PLLGNT 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	100/214 (47%)
		Neur_chan_memb	248..>359	CDD:280999	73/110 (66%)
		Neur_chan_memb	<683..760	CDD:280999	26/82 (32%)
Chrna6	NP_067344.2	LIC	15..484	CDD:273305	228/753 (30%)
		Neur_chan_LBD	34..240	CDD:280998	100/214 (47%)
		Neur_chan_memb	247..483	CDD:280999	121/518 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	399..423		7/87 (8%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2060
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H20888
Inparanoid	1	1.050	476	1.000	Inparanoid score	I1470
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm8871
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.870