Protein Alignment nAChRalpha3 and Chrna6

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_067344.2
• Gene: Chrna6 / 11440
• MGIID: 106213
• Length: 494
• Species: Mus musculus
• Alignment Length: 763
• Identity: 233/763 - (30%)
• Similarity: 333/763 - (43%)
• Gaps: 299/763 - (39%)

  Fly    14 LSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWV 78
            |:....||.....::|:..|.::||:.:|||.||:|.:||..:|.::||.:|:..||||.||||:
Mouse    22 LALFKGSTGCESEEQLFHRLFAHYNRFIRPVENVSDPVTVHFELAITQLANVDEVNQIMETNLWL 86

  Fly    79 EQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPP 143
            ...|.||:|:|:|.||.|:|.|.||:|:||:|||||||||.|:|:|...|||.|.|.|.:.|.||
Mouse    87 RHIWKDYRLRWDPTEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALLKYDGVITWTPP 151

  Fly   144 AIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWD 208
            ||:||||.:|:.:||||.|.|.:||||||||..::||.         ::...||:::|:.:.||:
Mouse   152 AIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLL---------IIGSKVDMNDFWENSEWE 207

  Fly   209 ILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKV 273
            |::....:::..|.||:|.|.|||::..:||..:|||:||||||:.|||||:||||||||.||||
Mouse   208 IVDASGYKHDIKYNCCEEIYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKV 272

  Fly   274 SLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTM 338
            :|.||:|||||||.|::.|.||.||||:||:|:::|||||..|.||.|||.|||||:|:|.||||
Mouse   273 TLCISVLLSLTVFLLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTM 337

  Fly   339 APWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSP 403
            ..||:|:|:...|..|:||:||
Mouse   338 PKWVKTIFLQAFPSILMMRKPL------------------------------------------- 359

  Fly   404 KTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQY 468
                                                                             
Mouse   360 ----------------------------------------------------------------- 359

  Fly   469 HNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSA 533
             ::.::.||  :..|.:.||
Mouse   360 -DKTKEAGG--VKDPKSHTK--------------------------------------------- 376

  Fly   534 NFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAAC 598
                                    .|..:|.|                       |:|
Mouse   377 ------------------------RPAKVKFT-----------------------HRG------- 387

  Fly   599 QSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAA 663
             .|:||       ::.||.|:..
Mouse   388 -ESKLL-------KECHHCQKSS------------------------------------------ 402

  Fly   664 GTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRW------HTCPELHKAMDGVTYIADQTR 722
                         ||.|..:|.         .....||      |: .::...::.|.:||:..:
Mouse   403 -------------DIAPGKRRS---------SQQPARWVAENSEHS-SDVEDVIESVQFIAENMK 444

  Fly   723 KEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDT 770
            ...|:..|::||||:|||:||:|||:|.|..|.||.|:.|| |.|.:|
Mouse   445 SHNETNEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVGLFLQ-PLLGNT 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	100/217 (46%)
		Cys-loop	150..164	CDD:349832	7/13 (54%)
		LGIC_TM_nAChR	242..>331	CDD:349866	63/88 (72%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	13/21 (62%)
		Neur_chan_memb	<683..760	CDD:460753	26/82 (32%)
Chrna6	NP_067344.2	LIC	15..484	CDD:455710	228/753 (30%)
		LGIC_ECD	34..240	CDD:475126	100/214 (47%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	399..423		7/87 (8%)