Protein Alignment nAChRalpha3 and CHRNB2

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_000739.1
• Gene: CHRNB2 / 1141
• HGNCID: 1962
• Length: 502
• Species: Homo sapiens
• Alignment Length: 760
• Identity: 244/760 - (32%)
• Similarity: 331/760 - (43%)
• Gaps: 297/760 - (39%)

  Fly    12 LVLSF----ISSSTANPDA-KRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKN 69
            |:|.|    :.|.....|. :||.:.||  |.||||:||..|.::.:||::.:.|:|||.|:.:.
Human    10 LLLGFGLLRLCSGVWGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHERE 74

  Fly    70 QIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNY 134
            ||||||:|:.|.|.||:|.|:|:|:..::.:.:||.|||.||:||||||||.:||:..:.|.::|
Human    75 QIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSY 139

  Fly   135 TGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLS 199
            .|.:.|.|||||||:|:|:|::||||:|.|.|||.|||||..::||....|:         ..|.
Human   140 DGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEV---------ASLD 195

  Fly   200 EFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFY 264
            :|..|.||||:.:|..|||   ...|..|:|||::..:|||.||||:||||||:.|:.|.|||||
Human   196 DFTPSGEWDIVALPGRRNE---NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFY 257

  Fly   265 LPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIH 329
            ||||.|||::|.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.|||:|
Human   258 LPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVH 322

  Fly   330 FRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPV 394
            .|||.||||||||:.||:.:||..|.|::|
Human   323 HRSPTTHTMAPWVKVVFLEKLPALLFMQQP----------------------------------- 352

  Fly   395 ALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTST 459
                .||..:...|     |:.|
Human   353 ----RHHCARQRLR-----LRRR------------------------------------------ 366

  Fly   460 TSSLIDAQYHNQYQQTG-GSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHH 523
                       |.::.| |:|.        ||:...
Human   367 -----------QREREGAGALF--------FREAPG----------------------------- 383

  Fly   524 LYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQI 588
                                                                  ||||   |..:
Human   384 ------------------------------------------------------ADSC---TCFV 391

  Fly   589 HQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAA 653
            ::
Human   392 NR--------------------------------------------------------------- 393

  Fly   654 AAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIA 718
            |:....|.|.|..|.|....|         |.|.|..|.              |.:|:|||.:||
Human   394 ASVQGLAGAFGAEPAPVAGPG---------RSGEPCGCG--------------LREAVDGVRFIA 435

  Fly   719 DQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            |..|.|::...|.|||||||||:||||||||....|.||.|:.||
Human   436 DHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQ 480

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	102/220 (46%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	59/88 (67%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	35/76 (46%)
CHRNB2	NP_000739.1	LIC	29..479	CDD:455710	237/738 (32%)
		LGIC_ECD_nAChR_B2	31..234	CDD:349826	100/214 (47%)
		Cys-loop	155..169	CDD:349826	8/13 (62%)