Protein Alignment nAChRalpha3 and CHRNB1

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_000738.2
• Gene: CHRNB1 / 1140
• HGNCID: 1961
• Length: 501
• Species: Homo sapiens
• Alignment Length: 744
• Identity: 204/744 - (27%)
• Similarity: 290/744 - (38%)
• Gaps: 289/744 - (38%)

  Fly    28 RLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEPK 92
            ||.:.|.|.|:..|||...|.|.:.|.:.|.|:|||.:|.|::.|:|.::::..|.||:|.|:|.
Human    29 RLREKLFSGYDSSVRPAREVGDRVRVSVGLILAQLISLNEKDEEMSTKVYLDLEWTDYRLSWDPA 93

  Fly    93 EYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEYF 157
            |:.|::.|.:.::.:|.||:||.||.||||:|.|.....::..|.|.|:||.||:|||.|.|.||
Human    94 EHDGIDSLRITAESVWLPDVVLLNNNDGNFDVALDISVVVSSDGSVRWQPPGIYRSSCSIQVTYF 158

  Fly   158 PFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFYT 222
            |||.|.|.|.|.|::||..:|.|:.....:|....|:.:....|..:.:|:|:..|:    :...
Human   159 PFDWQNCTMVFSSYSYDSSEVSLQTGLGPDGQGHQEIHIHEGTFIENGQWEIIHKPS----RLIQ 219

  Fly   223 CCDEP-------YLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISIL 280
            ...:|       ..::.|.:.:|||.|||.||:|.||:.|:.|.|.|||||.|:|||:.|||..|
Human   220 PPGDPRGGREGQRQEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLSIFAL 284

  Fly   281 LSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTV 345
            |:||||.||||:.:|.|||.||::.|:::|||:|.|||:.::|:|||:|.|||.||.|..|||.:
Human   285 LTLTVFLLLLADKVPETSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQMPLWVRQI 349

  Fly   346 FINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRST 410
            ||::||.:|.::||        |..|.||              |.||
Human   350 FIHKLPLYLRLKRP--------KPERDLM--------------PEPP------------------ 374

  Fly   411 SPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQT 475
                                          |.||
Human   375 ------------------------------HCSS------------------------------- 378

  Fly   476 GGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQ 540
                                      .|||.|.|...|                     |...|
Human   379 --------------------------PGSGWGRGTDEY---------------------FIRKP- 395

  Fly   541 HAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLH 605
                      .||.....||
Human   396 ----------PSDFLFPKPN--------------------------------------------- 405

  Fly   606 HQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPP 670
                                     |.....:|........||:.:.|
Human   406 -------------------------RFQPELSAPDLRRFIDGPNRAVA----------------- 428

  Fly   671 PPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWK 735
                                            ..|||.:.:..::|||.|.:::|:...:||||:
Human   429 --------------------------------LLPELREVVSSISYIARQLQEQEDHDALKEDWQ 461

  Fly   736 YVAMVLDRLFLWIFTIAVVVGTAGIILQA 764
            :||||:||||||.|.|...|||..|.|.|
Human   462 FVAMVVDRLFLWTFIIFTSVGTLVIFLDA 490

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	79/221 (36%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	52/88 (59%)
		TM1 helix	242..266	CDD:349866	14/23 (61%)
		TM2 helix	274..295	CDD:349866	13/20 (65%)
		TM3 helix	306..328	CDD:349866	11/21 (52%)
		Neur_chan_memb	<683..760	CDD:460753	27/76 (36%)
CHRNB1	NP_000738.2	LGIC_ECD_nAChR_B1	27..245	CDD:349825	78/219 (36%)
		Cys-loop	151..165	CDD:349825	9/13 (69%)
		Neur_chan_memb	252..487	CDD:460753	117/512 (23%)