Protein Alignment nAChRalpha3 and CHRNA7

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001177384.1
• Gene: CHRNA7 / 1139
• HGNCID: 1960
• Length: 531
• Species: Homo sapiens
• Alignment Length: 744
• Identity: 219/744 - (29%)
• Similarity: 308/744 - (41%)
• Gaps: 276/744 - (37%)

  Fly    27 KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEP 91
            ::||.:|:.|||.|.|||.|.:..|||...|.|.|::||:.|||::|||:|::.||.|:.|:|..
Human    56 RKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNV 120

  Fly    92 KEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEY 156
            .||.||:.:..|...||:|||:|||:||..|:.|..|...:|.:|..::.||.|:||||.|||.:
Human   121 SEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRW 185

  Fly   157 FPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFY 221
            ||||.|.|.:|||||:|.|:.:||:..:           .|:|.:..:.|||::.:|..|:|:||
Human   186 FPFDVQHCKLKFGSWSYGGWSLDLQMQE-----------ADISGYIPNGEWDLVGIPGKRSERFY 239

  Fly   222 TCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLSLTVF 286
            .||.|||.|:||.:||||:||:|.:||:|||:.||.|.:|||.||:|||||:||.|::|||||||
Human   240 ECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVF 304

  Fly   287 FLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLP 351
            .||:|||:|.||..|||:.::...|||:...|:.|||:||..|...|....|..|.|.:.:|...
Human   305 MLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCA 369

  Fly   352 RFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSPHLQH 416
            .||.|:||                    |   .|::.|                    ...|.|.
Human   370 WFLRMKRP--------------------G---EDKVRP--------------------ACQHKQR 391

  Fly   417 RVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGGSLLD 481
            |                 .|..:..:|::.||..|
Human   392 R-----------------CSLASVEMSAVAPPPAS------------------------------ 409

  Fly   482 HPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGY-GGHGQSGGHHLYRQQSSCSANFSPLPQHAHQA 545
                                      |||..| |..|..|.|        |
Human   410 --------------------------NGNLLYIGFRGLDGVH--------C-------------- 426

  Fly   546 HASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLH 610
               ..|.|.|:                   ......||.|                   |.:.|.
Human   427 ---VPTPDSGV-------------------VCGRMACSPT-------------------HDEHLL 450

  Fly   611 HQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGV 675
            |                                                   .|.||
Human   451 H---------------------------------------------------GGQPP-------- 456

  Fly   676 CDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMV 740
                     ||.                 |:|.|.::.|.|||::.|.::||..|..:||:.|.|
Human   457 ---------EGD-----------------PDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACV 495

  Fly   741 LDRLFLWIFTIAVVVGTAGIILQAPTLYD 769
            :|||.|..|::..::.|.||::.||...:
Human   496 VDRLCLMAFSVFTIICTIGILMSAPNFVE 524

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	99/215 (46%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	51/88 (58%)
		TM1 helix	242..266	CDD:349866	13/23 (57%)
		TM2 helix	274..295	CDD:349866	15/20 (75%)
		TM3 helix	306..328	CDD:349866	9/21 (43%)
		Neur_chan_memb	<683..760	CDD:460753	23/76 (30%)
CHRNA7	NP_001177384.1	LIC	3..519	CDD:273305	217/737 (29%)