Protein Alignment nAChRalpha3 and CHRNA4

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_000735.1
• Gene: CHRNA4 / 1137
• HGNCID: 1958
• Length: 627
• Species: Homo sapiens
• Alignment Length: 748
• Identity: 278/748 - (37%)
• Similarity: 371/748 - (49%)
• Gaps: 178/748 - (23%)

  Fly    27 KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEP 91
            :||...|.|.|||..|||.|::|.:.||..|.::|||||:.|||:||||:||:|.|:||||:|:|
Human    38 ERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 102

  Fly    92 KEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEY 156
            .:|..|..:.:||:.||||||||||||||:|.||..|||.|.:.|||:|.|||||||||.|||.:
Human   103 ADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTF 167

  Fly   157 FPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFY 221
            ||||:|.|.|||||||||..::||.::..         .||..:|:.|.||.|::.....|.:.|
Human   168 FPFDQQNCTMKFGSWTYDKAKIDLVNMHS---------RVDQLDFWESGEWVIVDAVGTYNTRKY 223

  Fly   222 TCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLSLTVF 286
            .||.|.|.|||:...:||..||||:||||||:.||.||:|||||||:.|||::|.||:|||||||
Human   224 ECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVF 288

  Fly   287 FLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLP 351
            .||:.||||.||||:||:|:::|||||..|.||.:||.|||:|.|||:||||..|||.||::.:|
Human   289 LLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVP 353

  Fly   352 RFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLP---PPVALSRMHHSPKTTSRSTSPH 413
            |.|:|:||     .::|.:.|.::.:.:.:....:..|.|   ||.
Human   354 RLLLMKRP-----SVVKDNCRRLIESMHKMASAPRFWPEPEGEPPA------------------- 394

  Fly   414 LQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGGS 478
                                     |....||:||:.|....
Human   395 -------------------------TSGTQSLHPPSPSFCVP----------------------- 411

  Fly   479 LLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPL-PQHA 542
             ||.|..|                                         ..||.:....| ||..
Human   412 -LDVPAEP-----------------------------------------GPSCKSPSDQLPPQQP 434

  Fly   543 HQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQ 607
            .:|..::.....|...|   ...|....:|.|.:|:....|...:..:|   |..|:|..:
Human   435 LEAEKASPHPSPGPCRP---PHGTQAPGLAKARSLSVQHMSSPGEAVEG---GVRCRSRSI---- 489

  Fly   608 QLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPP 672
                                .:..|...||..|...|||       |.|:....:|..|||..|.
Human   490 --------------------QYCVPRDDAAPEADGQAAG-------ALASRNTHSAELPPPDQPS 527

  Fly   673 AGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELH--------KAMDGVTYIADQTRKEEESTR 729
            ...|    .|::|  |...|....|:.|....|..|        :|::||.||||..:.|:....
Human   528 PCKC----TCKKE--PSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFS 586

  Fly   730 VKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIIL 762
            |||||||||||:||:|||:|.|..::||.|:.|
Human   587 VKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	113/215 (53%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	61/88 (69%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	14/20 (70%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	35/84 (42%)
CHRNA4	NP_000735.1	LGIC_ECD	37..243	CDD:475126	113/213 (53%)
		Neur_chan_memb	250..618	CDD:460753	159/524 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	382..481		28/213 (13%)
		PRK14959	<392..496	CDD:184923	30/242 (12%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..561		22/77 (29%)