Protein Alignment nAChRalpha3 and CHRNA2

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_000733.2
• Gene: CHRNA2 / 1135
• HGNCID: 1956
• Length: 529
• Species: Homo sapiens
• Alignment Length: 758
• Identity: 254/758 - (33%)
• Similarity: 327/758 - (43%)
• Gaps: 295/758 - (38%)

  Fly    19 SSTANPDA-------KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNL 76
            |.||.|..       .||:..|...||:..|||.|.:|.:.||..|.::|||||:.|||:||||:
Human    45 SPTALPQGGSHTETEDRLFKHLFRGYNRWARPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNV 109

  Fly    77 WVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWR 141
            |::|.|.||||:|.|.::|.:..|.|||:.||.|||||||||||.|.||..|||.|..||.|.|.
Human   110 WLKQEWSDYKLRWNPTDFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSTGTVHWV 174

  Fly   142 PPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVE 206
            |||||||||.|||.:||||:|.|.|||||||||..::||..:::.         |||.:::.|.|
Human   175 PPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMEQT---------VDLKDYWESGE 230

  Fly   207 WDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGE 271
            |.|:......|.|.|.||.|.|.|:|:...:||..||||:||||||:.||.||:||||||||.||
Human   231 WAIVNATGTYNSKKYDCCAEIYPDVTYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGE 295

  Fly   272 KVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTH 336
            |::|.||:|||||||.||:.||||.||||:||:|:::|||||..|.||.:||.|||:|.|||.||
Human   296 KITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTH 360

  Fly   337 TMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALS---R 398
            ||..|||...:..:||:|:|.||                               ||||.|.   |
Human   361 TMPHWVRGALLGCVPRWLLMNRP-------------------------------PPPVELCHPLR 394

  Fly   399 MHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSL 463
            :..||                :|:.::                                    |.
Human   395 LKLSP----------------SYHWLE------------------------------------SN 407

  Fly   464 IDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQ 528
            :||:......:.                                                  ..:
Human   408 VDAEEREVVVEE--------------------------------------------------EDR 422

  Fly   529 SSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMG 593
            .:|:.:.:|                                ||.|       .||
Human   423 WACAGHVAP--------------------------------SVGT-------LCS---------- 438

  Fly   594 AGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAA 658
                        |..||                                :.|.||...|..
Human   439 ------------HGHLH--------------------------------SGASGPKAEALL---- 455

  Fly   659 AAAAAGTPPPPPPPAGVCDILPACQREG----GPHCCSGRGNVRQRWHTCPELHKAMDGVTYIAD 719
                                     :||    .||                 :.||::||.||||
Human   456 -------------------------QEGELLLSPH-----------------MQKALEGVHYIAD 478

  Fly   720 QTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIIL 762
            ..|.|:..:.|||||||||||:||:|||:|.|...:||.|:.|
Human   479 HLRSEDADSSVKEDWKYVAMVIDRIFLWLFIIVCFLGTIGLFL 521

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	110/224 (49%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	62/88 (70%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	14/20 (70%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	34/80 (43%)
CHRNA2	NP_000733.2	Atrophin-1	<28..>75	CDD:460830	9/29 (31%)
		LIC	57..522	CDD:455710	250/746 (34%)
		LGIC_ECD_nAChR_A2	59..265	CDD:349816	110/214 (51%)
		Cys-loop	183..197	CDD:349816	9/13 (69%)