Protein Alignment nAChRalpha3 and chrna3l

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_031754974.1
• Gene: chrna3l / 101732933
• XenbaseID: XB-GENE-29086591
• Length: 498
• Species: Xenopus tropicalis
• Alignment Length: 761
• Identity: 238/761 - (31%)
• Similarity: 341/761 - (44%)
• Gaps: 291/761 - (38%)

  Fly    10 MILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTT 74
            :.|...|..|:..:    |||:|||.:||..:|||:||:|.:.:..::.:|||:.|:..||||.|
 Frog    25 LFLSAGFFCSNVEH----RLYEDLLKSYNPYIRPVMNVSDPVILHFEVSMSQLVKVDEVNQIMET 85

  Fly    75 NLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVE 139
            |||:...|.||||||.|.:|||:|.|.:|:..||||||||||||.|.|.|....||.:.|:|.|.
 Frog    86 NLWLRHLWNDYKLKWNPSDYGGIEFLRIPASQIWRPDIVLYNNAVGAFHVDDKAKAVVRYSGDVT 150

  Fly   140 WRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVG--VDLSEFY 202
            |.||||:||||:|||.:||||.|.|.:||||||||..::           |:|.:|  ::|.||:
 Frog   151 WIPPAIFKSSCKIDVTFFPFDYQNCSLKFGSWTYDKAKI-----------NLVMIGSKINLKEFW 204

  Fly   203 TSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPS 267
            .|.||.|:..|..::|..|.||.|.|.|||:::.:||..||||:.:||||:.||.||||||||||
 Frog   205 ESGEWVIINAPGYKHEIKYNCCVEIYQDITYSLYIRRLPLFYTIYIIIPCLLISCLTILVFYLPS 269

  Fly   268 DSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRS 332
            |.||||:|.:|:|||||||.|::.||||.||||:||:|:::|||||..|.||.:||.|||:|:|:
 Frog   270 DCGEKVTLCMSVLLSLTVFLLVITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRT 334

  Fly   333 PQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALS 397
            |:||||..||:..|::.||:.:.|.||                         ::.|
 Frog   335 PKTHTMPHWVKRFFLHLLPKVMFMTRP-------------------------EKQP--------- 365

  Fly   398 RMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSS 462
                                                                             
 Frog   366 ----------------------------------------------------------------- 365

  Fly   463 LIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQ 527
                               :.|.|..:..::|:..::.|:                    .|:.|
 Frog   366 -------------------YALKPQAYLDISSSEIRSCQD--------------------QLFYQ 391

  Fly   528 QSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGM 592
            .|||:.                          ..:|...|          :|:.|:.|    :|
 Frog   392 DSSCAC--------------------------GQLKRLYT----------SDTACNVT----RG- 415

  Fly   593 GAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAA 657
                  .|.|.:::..||
 Frog   416 ------SSLESMYNLGLH----------------------------------------------- 427

  Fly   658 AAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTR 722
                                              ||.|       ..||:.:|::.|.:||:..:
 Frog   428 ----------------------------------SGSG-------LSPEIREAIEDVHFIAENMK 451

  Fly   723 KEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLY 768
            .::|:..|::||||||||:||:|||:|.:..::||||:.|| |.|:
 Frog   452 AQDEAKEVQDDWKYVAMVIDRIFLWVFVLVCILGTAGLFLQ-PLLF 496

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	108/219 (49%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	60/88 (68%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	28/76 (37%)
chrna3l	XP_031754974.1	LIC	34..491	CDD:455710	232/744 (31%)
		LGIC_ECD_nAChR_A3	37..243	CDD:349817	108/220 (49%)
		Cys-loop	161..175	CDD:349817	9/13 (69%)