Protein Alignment nAChRalpha3 and LOC100487782

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_031759268.1
• Gene: LOC100487782 / 100487782
• XenbaseID: XB-GENE-29078911
• Length: 512
• Species: Xenopus tropicalis
• Alignment Length: 756
• Identity: 211/756 - (27%)
• Similarity: 311/756 - (41%)
• Gaps: 272/756 - (35%)

  Fly    10 MILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTT 74
            :.::|:....|......::||.:||.|||:|.|||||.:..|.|.::|.|.|:|||:.|||::.|
 Frog    18 IFVILTLPGGSHQGQYQRKLYRELLRNYNRLERPVVNDSQPLLVELQLTLLQIIDVDEKNQVLIT 82

  Fly    75 NLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVE 139
            |.|::.:|.|:.|.|:..||.||:.|..|:||||.|||:|||:||..|:.|..|...:|.||..:
 Frog    83 NAWLQITWNDFYLTWDQAEYPGVQTLRFPADHIWVPDILLYNSADERFDATFHTNVLVNSTGACQ 147

  Fly   140 WRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTS 204
            :.||.|.||:|.|||.:||||.|.|.:||||||::|:.:||:.::           .|:|.:..:
 Frog   148 YIPPGILKSTCYIDVRWFPFDVQKCKLKFGSWTHNGWLLDLQMME-----------ADISSYIPN 201

  Fly   205 VEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDS 269
            .|||::.||..|||.:|.||.|||.|:|:.:||||:||:|.:||:|||:.||.|.:|||.||:||
 Frog   202 GEWDLVGVPGKRNELYYECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADS 266

  Fly   270 GEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQ 334
            |||:||.|::|||||||.||:|||:|.||..|||:.::....|::...|:.||||||..|...||
 Frog   267 GEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASIMVIVGLSVVVTVLVLQFHHHDPQ 331

  Fly   335 THTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRM 399
            ...|...||.:.:|....||.||:|                    |...|    |:|........
 Frog   332 AGKMPKMVRVILLNWCAWFLRMRKP--------------------GENKR----PIPCKYIYPNH 372

  Fly   400 HHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLI 464
            |.|..:...:.:|                           ||               .||..:::
 Frog   373 HSSISSIEMNIAP---------------------------GH---------------QSTNGNIV 395

  Fly   465 DAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQS 529
            ...||.         |:.|..|
 Frog   396 YCGYHT---------LESPCCP------------------------------------------- 408

  Fly   530 SCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGA 594
                                ..||.|:....|                  :|.|
 Frog   409 --------------------PNSDSGVMCARV------------------NCPS----------- 424

  Fly   595 GAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAA 659
               .:.|:|:..:.|.
 Frog   425 ---LEDSDLIQKKSLK------------------------------------------------- 437

  Fly   660 AAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKE 724
                                                      .:.||:.|.::.|.|||.:.|::
 Frog   438 ------------------------------------------ESMPEIVKILEEVQYIARRFREQ 460

  Fly   725 EESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAP 765
            :....:..:||:.|.|:|||.|..|::..::.|..|::.||
 Frog   461 DAGEEICSEWKFAAAVIDRLCLVAFSLFAIIATFTILMSAP 501

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	102/217 (47%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	50/88 (57%)
		TM1 helix	242..266	CDD:349866	13/23 (57%)
		TM2 helix	274..295	CDD:349866	15/20 (75%)
		TM3 helix	306..328	CDD:349866	8/21 (38%)
		Neur_chan_memb	<683..760	CDD:460753	18/76 (24%)
LOC100487782	XP_031759268.1	LIC	16..500	CDD:455710	209/753 (28%)