DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nAChRalpha3 and chrna4

DIOPT Version :9

Sequence 1:	NP_001285015.1	Gene:	nAChRalpha3 / 31767	FlyBaseID:	FBgn0015519	Length:	795	Species:	Drosophila melanogaster
Sequence 2:	XP_031749826.1	Gene:	chrna4 / 100135106	XenbaseID:	XB-GENE-5994598	Length:	762	Species:	Xenopus tropicalis

Alignment Length:	767	Identity:	283/767 - (36%)
Similarity:	387/767 - (50%)	Gaps:	175/767 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 MILVLSFISSSTANPDAK-----RLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKN 69
            ::|:..|.|::..:.:.:     ||...|.|.|||..|||.|::||:.||..|.::|||||:.||
Frog   149 LLLLGGFFSTAFCHIETRAHAEERLLKGLFSGYNKWSRPVANISDAVMVRFGLSIAQLIDVDEKN 213

  Fly    70 QIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNY 134
            |:||||:||:|.|:||||:|:|.||..|..:.:||:.||||||||||||||:|.||..|||.|.:
Frog   214 QMMTTNVWVKQEWHDYKLRWDPLEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFH 278

  Fly   135 TGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDL----RHIDELNGTNVVEVG 195
            .||::|.|||||||||.|||.:||||:|.|.|||||||||..::||    .|:|:|
Frog   279 DGRIKWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDRAKIDLISMHSHVDQL--------- 334

  Fly   196 VDLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTI 260
                :::.|.||.|:......|.|.|.||.|.|.|||::..:||..||||:||||||:.||.||:
Frog   335 ----DYWESGEWVIVNAVGNYNIKKYECCTEIYSDITYSFIIRRLPLFYTINLIIPCLLISCLTV 395

  Fly   261 LVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLV 325
            |||||||:.|||::|.||:|||||||.||:.||||.||||:||:|:::|||||..|.||.:||.|
Frog   396 LVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFV 460

  Fly   326 LNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYP-ISEMIKSSRRLMVRTCNGLELRDQIPP 389
            ||:|.|||:||||..|||..|::.:||.|.|:||... ..::|:|   |..|:.|
Frog   461 LNVHHRSPRTHTMPAWVRRTFLDVVPRVLFMKRPAKDNCKKLIES---LHARSFN---------- 512

  Fly   390 LPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTAN 454
             |||...|.....|...|.|::                                       |..:
Frog   513 -PPPRLWSEAEIEPAFASSSSA---------------------------------------SPTS 537

  Fly   455 QQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQS 519
            |:.|.|||..                 |.|..:...|:...|                     .|
Frog   538 QRPSPTSSCC-----------------HQLEQSPNAQVFCKS---------------------PS 564

  Fly   520 GGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSG 584
            |.:.:..::|:.::..||.|:          |..|..|.||::....:| ||....|..::
Frog   565 GQYSMLCEESALTSCPSPKPE----------TKLLSEAQPNLMPKVRSV-SVQQMYTPKEA---- 614

  Fly   585 TIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPS 649
                ::|   ...|:|      :.:.:..:|....|...|
Frog   615 ----NEG---AVRCRS------RSIQYCFLHEDFSQANGQ------------------------- 641

  Fly   650 TSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWH----TCPELHKA 710
               |:::..|....|..|.|......|...|..| |.......|...|..:.|    ..|.|..|
Frog   642 ---ASSSPVAQKGNGNEPQPSSHTSPCTCKPKSQ-EKPVDPSQGAKTVNAKEHHVLLMSPSLKLA 702

  Fly   711 MDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIIL 762
            ::||.||||..|.|:....||:||||||||:||:|||:|.|..::||.|:.|
Frog   703 IEGVHYIADHLRAEDADFSVKQDWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 754

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	115/223 (52%)
		Neur_chan_memb	248..>359	CDD:280999	71/110 (65%)
		Neur_chan_memb	<683..760	CDD:280999	35/80 (44%)
chrna4	XP_031749826.1	LGIC_ECD	170..376	CDD:355788	115/218 (53%)
chrna4	XP_031749826.1	Neur_chan_memb	383..753	CDD:397193	159/517 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	252	1.000	Domainoid score	I2037
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	477	1.000	Inparanoid score	I1443
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm9533
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	6.060