Protein Alignment nAChRalpha3 and chrna4

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_031749826.1
• Gene: chrna4 / 100135106
• XenbaseID: XB-GENE-5994598
• Length: 762
• Species: Xenopus tropicalis
• Alignment Length: 767
• Identity: 283/767 - (36%)
• Similarity: 387/767 - (50%)
• Gaps: 175/767 - (22%)

  Fly    10 MILVLSFISSSTANPDAK-----RLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKN 69
            ::|:..|.|::..:.:.:     ||...|.|.|||..|||.|::||:.||..|.::|||||:.||
 Frog   149 LLLLGGFFSTAFCHIETRAHAEERLLKGLFSGYNKWSRPVANISDAVMVRFGLSIAQLIDVDEKN 213

  Fly    70 QIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNY 134
            |:||||:||:|.|:||||:|:|.||..|..:.:||:.||||||||||||||:|.||..|||.|.:
 Frog   214 QMMTTNVWVKQEWHDYKLRWDPLEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFH 278

  Fly   135 TGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDL----RHIDELNGTNVVEVG 195
            .||::|.|||||||||.|||.:||||:|.|.|||||||||..::||    .|:|:|
 Frog   279 DGRIKWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDRAKIDLISMHSHVDQL--------- 334

  Fly   196 VDLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTI 260
                :::.|.||.|:......|.|.|.||.|.|.|||::..:||..||||:||||||:.||.||:
 Frog   335 ----DYWESGEWVIVNAVGNYNIKKYECCTEIYSDITYSFIIRRLPLFYTINLIIPCLLISCLTV 395

  Fly   261 LVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLV 325
            |||||||:.|||::|.||:|||||||.||:.||||.||||:||:|:::|||||..|.||.:||.|
 Frog   396 LVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFV 460

  Fly   326 LNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYP-ISEMIKSSRRLMVRTCNGLELRDQIPP 389
            ||:|.|||:||||..|||..|::.:||.|.|:||... ..::|:|   |..|:.|
 Frog   461 LNVHHRSPRTHTMPAWVRRTFLDVVPRVLFMKRPAKDNCKKLIES---LHARSFN---------- 512

  Fly   390 LPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTAN 454
             |||...|.....|...|.|::                                       |..:
 Frog   513 -PPPRLWSEAEIEPAFASSSSA---------------------------------------SPTS 537

  Fly   455 QQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQS 519
            |:.|.|||..                 |.|..:...|:...|                     .|
 Frog   538 QRPSPTSSCC-----------------HQLEQSPNAQVFCKS---------------------PS 564

  Fly   520 GGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSG 584
            |.:.:..::|:.::..||.|:          |..|..|.||::....:| ||....|..::
 Frog   565 GQYSMLCEESALTSCPSPKPE----------TKLLSEAQPNLMPKVRSV-SVQQMYTPKEA---- 614

  Fly   585 TIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPS 649
                ::|   ...|:|      :.:.:..:|....|...|
 Frog   615 ----NEG---AVRCRS------RSIQYCFLHEDFSQANGQ------------------------- 641

  Fly   650 TSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWH----TCPELHKA 710
               |:::..|....|..|.|......|...|..| |.......|...|..:.|    ..|.|..|
 Frog   642 ---ASSSPVAQKGNGNEPQPSSHTSPCTCKPKSQ-EKPVDPSQGAKTVNAKEHHVLLMSPSLKLA 702

  Fly   711 MDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIIL 762
            ::||.||||..|.|:....||:||||||||:||:|||:|.|..::||.|:.|
 Frog   703 IEGVHYIADHLRAEDADFSVKQDWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 754

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	115/226 (51%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	61/88 (69%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	14/20 (70%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	35/80 (44%)
chrna4	XP_031749826.1	LGIC_ECD	170..376	CDD:475126	115/218 (53%)
		Neur_chan_memb	383..753	CDD:460753	159/517 (31%)