Protein Alignment nAChRalpha3 and chrnb2

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001093684.1
• Gene: chrnb2 / 100101688
• XenbaseID: XB-GENE-854457
• Length: 499
• Species: Xenopus tropicalis
• Alignment Length: 762
• Identity: 236/762 - (30%)
• Similarity: 323/762 - (42%)
• Gaps: 302/762 - (39%)

  Fly    10 MILVLSFISSSTANPDAKRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIM 72
            :.|:|..:.........:||.:.||  |.||||:||..|.::.:||::.:.|:|||.|:.:.|||
 Frog    13 LYLLLGLLPGCLGTDTEERLVEHLLDPSRYNKLIRPATNGSEQVTVQLMVSLAQLISVHEREQIM 77

  Fly    73 TTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGR 137
            |||:|:.|.|.||:|.|:|.|:..::.:.:||.|||.||:||||||||.:||:..:.|.:::.|.
 Frog    78 TTNVWLTQEWEDYRLTWDPAEFDDMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSHDGS 142

  Fly   138 VEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGV-DLSEF 201
            :.|.|||||||:|:|:|::||||:|.|.|||.|||||..::||          |:...| .|.:|
 Frog   143 IFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTELDL----------VLRSDVASLDDF 197

  Fly   202 YTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLP 266
            ..|.||||:.:|..|||   ...|..|:|||::..:|||.||||:||||||:.|:.|.|||||||
 Frog   198 TPSGEWDIIALPGRRNE---NPNDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLP 259

  Fly   267 SDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFR 331
            ||.|||::|.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.|||:|.|
 Frog   260 SDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHR 324

  Fly   332 SPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVAL 396
            ||.||||.|||:.||:::||..|.|::|.               :.|....||.|
 Frog   325 SPTTHTMPPWVKVVFLDKLPTLLFMKQPR---------------QNCARQRLRQQ---------- 364

  Fly   397 SRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTS 461
                                                                             
 Frog   365 ----------------------------------------------------------------- 364

  Fly   462 SLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYR 526
                   ....::..||..
 Frog   365 -------RQSQERAAGSFF---------------------------------------------- 376

  Fly   527 QQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQG 591
                                                              |.|...|.|..::|.
 Frog   377 --------------------------------------------------LRDGARSCTCYVNQA 391

  Fly   592 MGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAA 656
            .......|.|||
 Frog   392 SVKKYGAQLSEL----------------------------------------------------- 403

  Fly   657 AAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSG----RGNVRQRWHTC-PELHKAMDGVTY 716
                           |.||                :|    :|.|||    | ..|.:|:|||.:
 Frog   404 ---------------PEGV----------------NGFRDRQGKVRQ----CLCGLQEAVDGVRF 433

  Fly   717 IADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            |||..:.|::...|.|||||||||:||||||||....|.||.|:.||
 Frog   434 IADHMKSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQ 480

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	101/220 (46%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	59/88 (67%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	36/81 (44%)
chrnb2	NP_001093684.1	LIC	29..479	CDD:455710	232/743 (31%)
		LGIC_ECD_nAChR_B2	31..234	CDD:349826	100/215 (47%)
		Cys-loop	155..169	CDD:349826	8/13 (62%)