Protein Alignment nAChRalpha3 and chrnb1

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001240739.1
• Gene: chrnb1 / 100006941
• ZFINID: ZDB-GENE-070821-4
• Length: 492
• Species: Danio rerio
• Alignment Length: 763
• Identity: 214/763 - (28%)
• Similarity: 301/763 - (39%)
• Gaps: 288/763 - (37%)

  Fly     3 WFQVTIDMILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNL 67
            |..:|. .:..|..::.:|...|  :|...|.|.||..|||..|..:.:.|||.:.||..:.:|:
Zfish     6 WIIITC-CLCGLGAMTGATEMED--KLMKKLFSAYNSKVRPARNPEERVVVRIGMILSSFVSLNM 67

  Fly    68 KNQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATL 132
            |::.|.|.:.:...|.||:|.|.||:||||::|.:.|..:|.|||||.||.||.|.|.|......
Zfish    68 KDEEMNTIVMMNLEWNDYRLSWNPKDYGGVDVLRISSGKVWLPDIVLINNNDGVFGVALQVHVQA 132

  Fly   133 NYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVD 197
            ...|||.|.|||:|||||.:.|.|||||.|.|.|.|.|:|||..:|||:|..:..|..:.|:.:|
Zfish   133 YSNGRVTWTPPALYKSSCGVKVTYFPFDWQNCTMVFRSYTYDSSEVDLQHALDKRGKEIKEIILD 197

  Fly   198 LSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILV 262
             :.|..|.||.|....:.:|.:     ::.|.||||.:.:.||.:||.||:::||:.|:.:.|..
Zfish   198 -TGFSESGEWHIRHKVSRKNVR-----EDLYEDITFYLIIERKPMFYVVNIVLPCILITIIAIFN 256

  Fly   263 FYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLN 327
            ||||.|:|||:.|||::||:||||.||||..||.|||.||::..:::.||||.|||:.::|:|||
Zfish   257 FYLPPDAGEKMGLSINVLLTLTVFLLLLANKIPETSLGVPIIVNYLMITMILVTFSVILSVVVLN 321

  Fly   328 IHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPP 392
            :|.|||.||.|..|||.:||:.||.:|.|.||              .|.|
Zfish   322 LHHRSPNTHQMPLWVRKIFIHMLPPYLGMLRP--------------KVET--------------- 357

  Fly   393 PVALSRMHHSPKTTSRSTSPHLQHRVQTYN-LMDECGMGMGGGASTITGHLSSLYPPTMSTANQQ 456
            |:.|.:             |..:..:|..| :.||
Zfish   358 PLFLEK-------------PAKKENIQAINRVADE------------------------------ 379

  Fly   457 TSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGG 521
                                                                             
Zfish   380 ----------------------------------------------------------------- 379

  Fly   522 HHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTI 586
             :..|:.|:.:..| |.| |.:|                                 .|..|:...
Zfish   380 -YFIRKPSNMNFLF-PKP-HRYQ---------------------------------PDGQCTDLR 408

  Fly   587 QIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTS 651
            :...|                                 ..|:...|
Zfish   409 KFIDG---------------------------------PSHYLTLP------------------- 421

  Fly   652 AAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTY 716
                                                                 |||..|::.:||
Zfish   422 -----------------------------------------------------PELKTAVEAITY 433

  Fly   717 IADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQA 764
            ||:|.:.|::...:||||:|||||.||||||||.|...:||.||...|
Zfish   434 IAEQLQAEKDYEALKEDWQYVAMVADRLFLWIFVIFTTLGTLGIFTDA 481

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	91/217 (42%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	48/88 (55%)
		TM1 helix	242..266	CDD:349866	10/23 (43%)
		TM2 helix	274..295	CDD:349866	13/20 (65%)
		TM3 helix	306..328	CDD:349866	10/21 (48%)
		Neur_chan_memb	<683..760	CDD:460753	30/76 (39%)
chrnb1	NP_001240739.1	LGIC_ECD	26..235	CDD:475126	90/216 (42%)
		Neur_chan_memb	242..478	CDD:460753	113/513 (22%)