Protein Alignment nAChRalpha3 and chrnb1l

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_068078213.1
• Gene: chrnb1l / 100005295
• ZFINID: ZDB-GENE-060811-1
• Length: 489
• Species: Danio rerio
• Alignment Length: 760
• Identity: 209/760 - (27%)
• Similarity: 310/760 - (40%)
• Gaps: 289/760 - (38%)

  Fly    17 ISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQS 81
            ::.:.|:...:||.:||..:||..|||.....:.:.||:.:.|.|||.:|.||..||||:::...
Zfish    14 LALTEASEAERRLLEDLFQDYNLKVRPARTWNERVMVRVGMTLVQLISLNQKNGEMTTNVFMNME 78

  Fly    82 WYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIY 146
            |.||:|.|.|::|..::::.:|...:|||||.|.||.||.|:|.|.....:...|.|.|.|||||
Zfish    79 WTDYRLSWNPEDYDKIDVVRIPPVKVWRPDIYLINNNDGQFDVALYVNVLVRSDGTVSWLPPAIY 143

  Fly   147 KSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRH-IDELNGTNVVEVGVDLSEFYTSVEWDIL 210
            :|||.|:|.|||||.|.|.|.|.|:|||..:|||:: :|| :|..:.|:.:|.:.|..:.||.|.
Zfish   144 RSSCSIEVAYFPFDWQNCSMVFRSYTYDSSEVDLQYGLDE-DGNELHEIVIDENAFTENGEWQIC 207

  Fly   211 EVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSL 275
            ..|:.:|.:     |:.|.||||.:.:.||.|||.:|:|:||:..|.|.|.|||||..:|||::|
Zfish   208 HKPSRKNVQ-----DDLYEDITFYLIIERKPLFYVINIIVPCILTSVLAIFVFYLPPGAGEKMTL 267

  Fly   276 SISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAP 340
            |||:|::||||.||||:.:|.|||.:|::..:|:|||||.|||:.::|:|||:|.|:|.||.|..
Zfish   268 SISVLIALTVFMLLLADKVPETSLAIPIIVNYVMFTMILVTFSVILSVVVLNLHHRTPSTHHMPG 332

  Fly   341 WVRTVFINQLPRFLVMRRPL--YPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSP 403
            |||.||||.|||:|.:.||.  .|:.|                |.::.:|
Zfish   333 WVRKVFINLLPRYLGVLRPTPEEPVEE----------------EPKENVP--------------- 366

  Fly   404 KTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQY 468
                                     ||..|         :||.|
Zfish   367 -------------------------MGCLG---------NSLRP--------------------- 376

  Fly   469 HNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSA 533
                                                               ||.:..|:
Zfish   377 ---------------------------------------------------GGEYFIRK------ 384

  Fly   534 NFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAAC 598
                                   .||
Zfish   385 -----------------------INP--------------------------------------- 387

  Fly   599 QSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAA 663
               ||:...:..|.:...:.|:.....::
Zfish   388 ---ELVMPWRGSHNESTVKLQRFSNSDNY------------------------------------ 413

  Fly   664 GTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEEST 728
                        |.|||                        |.|..|:..:||:::|.:|::...
Zfish   414 ------------CLILP------------------------PNLKSAIAAITYMSEQLKKQDVDD 442

  Fly   729 RVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMP 773
            .:.:||:|:|:||||||||:|.:...:||..:.|.|...|....|
Zfish   443 TMTDDWQYIAVVLDRLFLWLFVVITTLGTLTMFLDASFNYTPDQP 487

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	91/218 (42%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	50/88 (57%)
		TM1 helix	242..266	CDD:349866	13/23 (57%)
		TM2 helix	274..295	CDD:349866	13/20 (65%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	22/76 (29%)
chrnb1l	XP_068078213.1	LIC	2..476	CDD:455710	205/747 (27%)
		LGIC_ECD_nAChR_B1	23..233	CDD:349825	90/215 (42%)
		Cys-loop	147..161	CDD:349825	9/13 (69%)