Protein Alignment nAChRalpha3 and chrnb5a

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_021332008.1
• Gene: chrnb5a / 100003503
• ZFINID: ZDB-GENE-090312-169
• Length: 494
• Species: Danio rerio
• Alignment Length: 760
• Identity: 233/760 - (30%)
• Similarity: 322/760 - (42%)
• Gaps: 295/760 - (38%)

  Fly    17 ISSSTANPDAKRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVE 79
            ::.|.|....:||.|.||  ..||||:||.||.:..:|:.||:.|:|||.||.:.||||||:|:.
Zfish    20 VARSLAADAEERLVDFLLGPERYNKLIRPAVNKSQQVTIGIKVSLAQLISVNEREQIMTTNVWLT 84

  Fly    80 QSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPA 144
            |.|.||:|.|:|.||.|::.|.:||.|||.|||||||||||.:||:....|.::.||.:.|.|||
Zfish    85 QEWNDYRLVWDPNEYEGIKKLRIPSRHIWLPDIVLYNNADGVYEVSFYCNAVVSNTGDIFWLPPA 149

  Fly   145 IYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDI 209
            ||||:|.|:|..||||:|.|.:||.|||||..::||....:.         ....::..|.||||
Zfish   150 IYKSACAIEVRNFPFDQQNCTLKFRSWTYDRTELDLYLTSDF---------ASRDDYTPSGEWDI 205

  Fly   210 LEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVS 274
            :.:|..|||..   .|..|||:|::..::||.||||:||||||:.|:.|.|||||||||.|||::
Zfish   206 VSLPGRRNEDL---SDPTYLDVTYDFVIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMT 267

  Fly   275 LSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMA 339
            |.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.|||:|.|||.||.|.
Zfish   268 LCISVLLALTVFLLLISKIVPPTSLAVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHRMP 332

  Fly   340 PWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPK 404
            .||:.||:::||.||:||||                   ....:|::.          |..|..|
Zfish   333 EWVKRVFLHKLPFFLLMRRP-------------------GRSNVRERF----------RRKHQRK 368

  Fly   405 TTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYH 469
            :.|..|.                |:.:.|                               |:.:
Zfish   369 SFSSDTK----------------GLRLDG-------------------------------DSYF- 385

  Fly   470 NQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSAN 534
                     |.|.|                       |...|.:                    .
Zfish   386 ---------LSDDP-----------------------GRVCGAW--------------------R 398

  Fly   535 FSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQ 599
            ...||                                                           :
Zfish   399 VGDLP-----------------------------------------------------------E 404

  Fly   600 SSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAG 664
            .||.....::.|.
Zfish   405 GSEFRQRVKVRHD---------------------------------------------------- 417

  Fly   665 TPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTR 729
                                                    |::.:|::||.:||:..:.|::...
Zfish   418 ----------------------------------------PDVDEAIEGVRFIAEHMKIEDDDEG 442

  Fly   730 VKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPI 774
            :.|||||||||:||||||||.:..||||.|:.:| |.......|:
Zfish   443 IIEDWKYVAMVIDRLFLWIFILVCVVGTLGLFVQ-PLFQSYNTPV 486

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	103/219 (47%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	59/88 (67%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	28/76 (37%)
chrnb5a	XP_021332008.1	LIC	20..474	CDD:455710	230/745 (31%)
		LGIC_ECD	31..234	CDD:475126	102/214 (48%)