DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Gbeta5 and STE4

DIOPT Version :9

Sequence 1:	NP_572452.1	Gene:	Gbeta5 / 31744	FlyBaseID:	FBgn0030011	Length:	358	Species:	Drosophila melanogaster
Sequence 2:	NP_014855.3	Gene:	STE4 / 854387	SGDID:	S000005738	Length:	423	Species:	Saccharomyces cerevisiae

Alignment Length:	403	Identity:	135/403 - (33%)
Similarity:	219/403 - (54%)	Gaps:	56/403 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 PASNANANATE-----KMASLVREAENLKTKLEEERQKLNDVNLSNIAERLEQIA--YVNIKPRK 64
            |.|..:.:|.|     |:.:..:|::.|..::.:.:.|:.|.:|..:|.::..:.  .:|:||..
Yeast    22 PQSLQDISAVEDEIQNKIEAARQESKQLHAQINKAKHKIQDASLFQMANKVTSLTKNKINLKPNI 86

  Fly    65 VLKGHQAKVLCTDWSPDKRHIISSSQDGRLIIWDAFTTNKEHAVTMPTTWIMACAYAPSGNFVAC 129
            |||||..|:....||.|.:.|:|:||||.::|||:.:..|::|:.:.:.|:::||.:||...||.
Yeast    87 VLKGHNNKISDFRWSRDSKRILSASQDGFMLIWDSASGLKQNAIPLDSQWVLSCAISPSSTLVAS 151

  Fly   130 GGLDNKVTVYPITSDEEMAAKKRTV-GTHTSYMSCCIYPNSDQQILTGSGDSTCALWDVESGQLL 193
            .||:|..|:|.::.:..:|....:: ..||.|:|...: ..:..|||.|||.||||||:...:.:
Yeast   152 AGLNNNCTIYRVSKENRVAQNVASIFKGHTCYISDIEF-TDNAHILTASGDMTCALWDIPKAKRV 215

  Fly   194 QSFHGHSGDVMAIDL--APN--ETGNTFVSGSCDRMAFIWDMRSGHVVQSFEGHQSDVNSVKFHP 254
            :.:..|.|||:|:.:  .||  .:.|||.|...|...:|||.||...||||..:.||:|:::|..
Yeast   216 REYSDHLGDVLALAIPEEPNSENSSNTFASCGSDGYTYIWDSRSPSAVQSFYVNDSDINALRFFK 280

  Fly   255 CGDAIATGSDDSSCRLYDMRADREVAVFAKESIIF------------------------------ 289
            .|.:|..|||:.:..:||:|:|..:|.|:    :|
Yeast   281 DGMSIVAGSDNGAINMYDLRSDCSIATFS----LFRGYEERTPTPTYMAANMEYNTAQSPQTLKS 341

  Fly   290 ---------GVNSVDFSVSGRLLFAGYNDYTVNLWDTLKSERVCLLYGHENKVSCVQVSPDGTAL 345
                     ||.|:|||.||||:::.|.|....:||.||.|.|..|.||..:|:.|:.||||.|:
Yeast   342 TSSSYLDNQGVVSLDFSASGRLMYSCYTDIGCVVWDVLKGEIVGKLEGHGGRVTGVRSSPDGLAV 406

  Fly   346 STGSWDYTIRVWA 358
            .|||||.|:::|:
Yeast   407 CTGSWDSTMKIWS 419

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Gbeta5	NP_572452.1	WD40	62..358	CDD:238121	121/339 (36%)
		WD40 repeat	73..110	CDD:293791	14/36 (39%)
		WD40 repeat	116..156	CDD:293791	12/40 (30%)
		WD40 repeat	161..197	CDD:293791	13/35 (37%)
		WD40 repeat	204..241	CDD:293791	16/40 (40%)
		WD40 repeat	247..283	CDD:293791	12/35 (34%)
		WD40 repeat	291..327	CDD:293791	17/35 (49%)
		WD40 repeat	333..357	CDD:293791	13/23 (57%)
STE4	NP_014855.3	WD40	87..419	CDD:238121	120/336 (36%)
		WD40 repeat	95..132	CDD:293791	14/36 (39%)
		WD40 repeat	138..179	CDD:293791	12/40 (30%)
		WD40 repeat	184..218	CDD:293791	13/34 (38%)
		WD40 repeat	226..266	CDD:293791	15/39 (38%)
		WD40 repeat	273..329	CDD:293791	14/59 (24%)
		WD40 repeat	352..388	CDD:293791	17/35 (49%)
		WD40 repeat	394..418	CDD:293791	13/23 (57%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346073
Domainoid	1	1.000	47	1.000	Domainoid score	I3018
eggNOG	1	0.900	-	-		E1_KOG0286
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H40714
Inparanoid	1	1.050	238	1.000	Inparanoid score	I698
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000271
OrthoInspector	1	1.000	-	-		otm46827
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR19850
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X182
TreeFam	1	0.960	-	-
	11	10.850