Protein Alignment Upf2 and NMD2

DIOPT Version :10

• Sequence 1: NP_572434.1
• Gene: Upf2 / 31724
• FlyBaseID: FBgn0029992
• Length: 1241
• Species: Drosophila melanogaster
• Sequence 2: NP_011944.2
• Gene: NMD2 / 856476
• SGDID: S000001119
• Length: 1089
• Species: Saccharomyces cerevisiae
• Alignment Length: 1236
• Identity: 272/1236 - (22%)
• Similarity: 482/1236 - (38%)
• Gaps: 307/1236 - (24%)

  Fly    93 GEEYF----AKLDSNLKKNTAFVKKLKL-FTATQLDGLLREISALNLSKYISEICAALVEAKL-- 150
            |||.|    .||||::|:||.|:||||. |.......||:::|..:|.||:|||...:.|..|
Yeast    19 GEEVFPLKSKKLDSSIKRNTGFIKKLKKGFVKGSESSLLKDLSEASLEKYLSEIIVTVTECLLNV 83

  Fly   151 --KMTDVPAVVTLASRLHCTY-----ADFDVHFLEAWQK-ALNIKA-TEKIGNPSKLRVDLRLFA 206
              |..||.|.|.:.|.||..:     :.....||:|::. :::|:: .:::...::::.:||:|.
Yeast    84 LNKNDDVIAAVEIISGLHQRFNGRFTSPLLGAFLQAFENPSVDIESERDELQRITRVKGNLRVFT 148

  Fly   207 ELVSSGV----------------IQMKPGLAQ--LGVVLVHLIALDKDDHSNFSIILSFCRHCGE 253
            ||...||                :|.|.|...  |..:|..::        |:...|.|......
Yeast   149 ELYLVGVFRTLDDIESKDAIPNFLQKKTGRKDPLLFSILREIL--------NYKFKLGFTTTIAT 205

  Fly   254 EYAGLVPQKMQQLATKYGVEVPKSDFLTADKQLNLRTMLKGYFKALCKHVLAEQAELMNMTKNIR 318
            .:       :::.|..:..:....|.|..|.:  |:..|:..||.......|...||......::
Yeast   206 AF-------IKKFAPLFRDDDNSWDDLIYDSK--LKGALQSLFKNFIDATFARATELHKKVNKLQ 261

  Fly   319 RT-MECK---GEISTEKREKCELMQAGFDKLLASAQSLSELLGEPLPELAKESEGCNPGTVIDNM 379
            |. .:|:   |::..|..|:.:.:...|.:...||.:|.|.....:|||    ||.:.    |::
Yeast   262 REHQKCQIRTGKLRDEYVEEYDKLLPIFIRFKTSAITLGEFFKLEIPEL----EGASN----DDL 318

  Fly   380 LDSAS----FGVLDP----WGDEETRAFYTDLPDLRQFLPNFSAPKVDLETLEEPSELTEEAIDA 436
            .::||    ..:|.|    |.:|:||.||..|||:.             :|:||
Yeast   319 KETASPMITNQILPPNQRLWENEDTRKFYEILPDIS-------------KTVEE----------- 359

  Fly   437 NLDAEMDLDDPPSTTSDTTPENPIEEQPTTPVAAAEDLKPQKMGNALMELGRQQQQQSQLNQNPI 501
                        |.:|.|                                    ::.|.:|...|
Yeast   360 ------------SQSSKT------------------------------------EKDSNVNSKNI 376

  Fly   502 QIQNQMRQQFDGFLVNLFNCVNKELIDSAAIEFLLNF-NTKHQRKKLTRTIFSVQRTRLDILPYL 565
            .:          |..:|.....|::||..:..:..:: :.|..|.::.:  |.::......||..
Yeast   377 NL----------FFTDLEMADCKDIIDDLSNRYWSSYLDNKATRNRILK--FFMETQDWSKLPVY 429

  Fly   566 SRFVAIVHMCNTDVATDLAELLRKEFKWHIHKKNQLNIETKLKIVRFIGELVKFGLFKKFDALGC 630
            |||:|.......::.::....|...|:..:| .|::|:    |.:.|..|::||.|...|.....
Yeast   430 SRFIATNSKYMPEIVSEFINYLDNGFRSQLH-SNKINV----KNIIFFSEMIKFQLIPSFMIFHK 489

  Fly   631 LKMLLRDFQ-HHQIEMACAFVEVTGVYLYNCRDARLLMNVFLDQMLRLKTATAMDSRHAAQIESV 694
            ::.|:...| .:.:|:....:|.:|.:|.|..:.:.||...:..:...|....::....:.:|::
Yeast   490 IRTLIMYMQVPNNVEILTVLLEHSGKFLLNKPEYKELMEKMVQLIKDKKNDRQLNMNMKSALENI 554

  Fly   695 YYLVKPPESSKREPMVRPAMHE--YIRYLIFEELCKQNVDRCIKMLRRIDWQDPETNCYAIKCLS 757
            ..|:.||........|:....|  :.|.||..||...:....:|::|:..|.|...........|
Yeast   555 ITLLYPPSVKSLNVTVKTITPEQQFYRILIRSELSSLDFKHIVKLVRKAHWDDVAIQKVLFSLFS 619

  Fly   758 KAYLLRFQLIRCLADLVSGLSSYQPRAVTIVIDNVFEDIRAGLEIHSPRMAQRRIAMAKYLGEMY 822
            |.:.:.:|.|..|..::.||.||:...|...||.|.|:|..||||:.......||:..:||.|::
Yeast   620 KPHKISYQNIPLLTKVLGGLYSYRRDFVIRCIDQVLENIERGLEINDYGQNMHRISNVRYLTEIF 684

  Fly   823 NYKLVESTNILNTLYSIISLG-VSNDQN--VVSPLDPPDSLFRLKLACMLLDTCAPYFTSQATRK 884
            |:::::|..:|:|:|.||..| ::|..|  .::..||||:.||::|...:|.....  |..|..|
Yeast   685 NFEMIKSDVLLDTIYHIIRFGHINNQPNPFYLNYSDPPDNYFRIQLVTTILLNINR--TPAAFTK 747

  Fly   885 KLDYFLVFFQHYYWFKKSHPVFSKTE----NTSDLFPILMDHT---YRDCLLNVRPKLK-IYKSL 941
            |....|.||: ||.|.|..|:..:||    :|...:..:..:|   ..:.|:....:|: :.|||
Yeast   748 KCKLLLRFFE-YYTFIKEQPLPKETEFRVSSTFKKYENIFGNTKFERSENLVESASRLESLLKSL 811

  Fly   942 EQAK----------AAIDHLQEKLYP----------------------------QLKTTNNAQDP 968
            ...|          |:|.:.:|...|                            ::.|.|....|
Yeast   812 NAIKSKDDRVKGSSASIHNGKESAVPIESITEDDEDEDDENDDGVDLLGEDEDAEISTPNTESAP 876

  Fly   969 SLGTISEISEIDEGGTDEDSGSSNDQRERQVSGQEQDQ----------SNDWTENEAEPPLP--- 1020
            ......:....||...|:|....:|..:....|:|.|:          .:|..|.:::..|.
Yeast   877 GKHQAKQDESEDEDDEDDDEDDDDDDDDDDDDGEEGDEDDDEDDDDEDDDDEEEEDSDSDLEYGG 941

  Fly  1021 -------------------PLPPPEKSKEDLEFEQLYEKMTTDSYQERLKEPIKATAKDIPV--- 1063
                               .|...|:.|.:.|.|:.::||..:|...|..|  |..|..|||
Yeast   942 DLDADRDIEMKRMYEEYERKLKDEEERKAEEELERQFQKMMQESIDARKSE--KVVASKIPVISK 1004

  Fly  1064 PMMAR---LQKKSYDQITGAQTIGPGINNMSQISKSVPNQDGTPGSPGADSPDGNGKSGGGVGGS 1125
            |:..:   |.|||.:..:..:|       ..::||  |.:
Yeast  1005 PVSVQKPLLLKKSEEPSSSKET-------YEELSK--PKK------------------------- 1035

  Fly  1126 DVGAAGAAGASGATSVPFVLMVRGHKGGKQQFKSFVAPSDSHLATNLKRQEQMIREEKEKVKRLT 1190
                           :.|..:.:  .|.|.|.:....|:|....:::..:|:.::.|:.|:|::.
Yeast  1036 ---------------IAFTFLTK--SGKKTQSRILQLPTDVKFVSDVLEEEEKLKTERNKIKKIV 1083

  Fly  1191 L 1191
            |
Yeast  1084 L 1084

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Upf2	NP_572434.1	MIF4G	104..300	CDD:397130	55/225 (24%)
		MIF4G	518..701	CDD:214713	37/184 (20%)
		MIF4G	716..934	CDD:397130	69/229 (30%)
		Upf2	1029..1192	CDD:461146	33/169 (20%)
NMD2	NP_011944.2	MIF4G	34..241	CDD:397130	54/223 (24%)
		MIF4G	374..561	CDD:214713	39/203 (19%)
		MIF4G	578..779	CDD:397130	65/203 (32%)
		Upf2	976..1084	CDD:461146	29/160 (18%)