Protein Alignment Upf2 and smg-3

DIOPT Version :9

• Sequence 1: NP_572434.1
• Gene: Upf2 / 31724
• FlyBaseID: FBgn0029992
• Length: 1241
• Species: Drosophila melanogaster
• Sequence 2: NP_001368064.1
• Gene: smg-3 / 177397
• WormBaseID: WBGene00004881
• Length: 1144
• Species: Caenorhabditis elegans
• Alignment Length: 1223
• Identity: 343/1223 - (28%)
• Similarity: 578/1223 - (47%)
• Gaps: 157/1223 - (12%)

  Fly    68 FISELREKIESKRQLRLQNSTIE---LPGEEYFAKLDSNLKKNTAFVKKLKLFTATQLDGLLREI 129
            ::.||:::::.:...|||...|.   |..|:....|||.|||.|:|:||:||.:|..:..|:.|:
 Worm     9 WLEELKKRVKYQETSRLQMVEIRETLLDNEKELRSLDSTLKKTTSFMKKVKLLSAATVPQLIEEL 73

  Fly   130 SALNLSKYISEICAALVEAKLKMTDVPAVVTLASRLHCTYADFDVHFLEAWQKALNIKATEKIGN 194
            |.|||||::.|:.:.::|.|||::|:|.|..|...:...|::|.......::|.|.:|.::||.|
 Worm    74 SKLNLSKFVEEMASGIIETKLKLSDIPKVTELCLAISAKYSNFSEQMAGEFKKVLPVKKSDKIAN 138

  Fly   195 PSKLRVDLRLFAELVSSGVIQMKPGLAQLGVVLVHLIALDKDDHSNFSIILSFCRHCGEEYAGLV 259
            .:|||||:...|||...||...|.||..||.||.:||..||.|..|..::.:..|..|.:.|.:|
 Worm   139 VAKLRVDIIFLAELCLCGVFNEKEGLQVLGAVLSYLIQTDKTDFVNVGLLATVSRTVGWQIANIV 203

  Fly   260 PQKMQQLATK--YGVEVPKSDFLTADKQLNLRTMLKGYFKALCKHVLAEQAELMNMTKNIRRTME 322
            |..|....|.  ...|:|:|..|:.:::.::|.:.|.|:.:|........:......|.::|...
 Worm   204 PIPMDDAETVEIQDEELPESSALSVEQKKSIRELFKSYYDSLYSKTEKTCSARNKAMKRVKRQER 268

  Fly   323 CKGEISTEKREKCELMQAGFDKLLASAQSLSELLG---EPLPELAKESEGCNPGTVIDNMLDSAS 384
            .:|:...|::.....:|:..|.|...|..:|..:|   :||.|.|.:.|......:  .|....:
 Worm   269 SRGDAGDEEKTTLNDLQSELDSLRKMAIEISSAIGVEMKPLKEEASDDEEDEAANL--EMGRKLA 331

  Fly   385 FGVLDPWGDEETRAFYTDLPDLRQFLPNFSAPKVDLETLEEP--SELTEEAIDANLDAEMDLDDP 447
            .|.:..|.||||:|||.||.||||.:|.....:.:..||.:.  :|..|:....|::....:|..
 Worm   332 EGAIKLWSDEETKAFYEDLIDLRQMVPKDLYKESEQRTLSKAKMAERIEDVDMENINESGAMDAK 396

  Fly   448 PSTTSDTTPENPIEEQPTTPVAAAEDLKPQKMGNALMELGRQQQQQSQLNQNPIQIQNQMRQQFD 512
            .|:.     :....|:.|||    ||                .|.|:.|.:.....:.:...::.
 Worm   397 RSSL-----QRGESEKETTP----ED----------------SQLQALLKEAVDASEERGVSKWQ 436

  Fly   513 GFLVNLFNCVNKELIDSAAIEFLLNFNTKHQRKKLTRTIFSVQRTRLDILPYLSRFVAIVHMCNT 577
            .|:::|.:.|:|...|.|||.|:.|.|.|..||:|.:.:.....:|:|::|:.:|.||.:.....
 Worm   437 SFVLDLDHLVSKYSTDQAAIYFVSNLNNKGCRKRLVKLMIDPPPSRIDVVPFYARLVATLENVMP 501

  Fly   578 DVATDLAELLRKEFKWHIHKKNQ-----LNIETKLKIVRFIGELVKFGLFKKFDALGCLKMLLRD 637
            |:.|::...|.::|:..:.:|..     :.:|:|:..|..|.||:|||:..:.:.|.||:.|:.|
 Worm   502 DLTTEIVTQLLEKFRGFLQQKPSSATAIIKVESKMVCVMMIAELMKFGVISRAEGLSCLRQLVYD 566

  Fly   638 FQHHQIEMACAFVEVTGVYLYNCRDARLLMNVFLDQMLRLKTATAMDSRHAAQIESVYYLVKPPE 702
            .:.|.:||...|:|.:|:|||...::...|...| ::::.|.....|.|.|..|::.|:...|||
 Worm   567 LRGHSVEMTATFMESSGLYLYRHTESHAKMKRLL-EVVKAKRERMKDQRQAMLIDNAYFTCLPPE 630

  Fly   703 SSKRE-----------PMVRPAMHEYIRYLIFEELCKQNVDRCIKMLRRIDWQDPETNCYAIKCL 756
            .||.|           ||.|     :||::|. ::.:..||..:|.|||::|.|||...|||:.|
 Worm   631 DSKEERLRLKLDEEDTPMKR-----FIRHIIL-DINESTVDVFLKCLRRLEWYDPEVADYAIRYL 689

  Fly   757 SKAYLLRFQLIRCLADLVSGLSS--YQPRAVTIVIDNVFEDIRAGLEIHSPRM-AQRRIAMAKYL 818
            |..:||..:.::.:|..::||.:  :.......|||:..|.||..||  :|.: .|...:.|.||
 Worm   690 SSTWLLPIENLQHVASAIAGLCNLVHLQWIGMAVIDSTIETIRISLE--NPGVYNQWAHSAAVYL 752

  Fly   819 GEMYNYKLVESTNILNTLYSIISLGVSNDQNVVSPLDPPDS---LFRLKLACMLLDTCAPYFTSQ 880
            .|:|:::|.:...:|..||.:||..           :|.:|   |.|:::.|.:|:....:|...
 Worm   753 AELYSFELCDEDLVLKILYQLISYP-----------EPENSWRDLHRIRMICAMLEILREFFMKG 806

  Fly   881 ATRKKLDYFLVFFQHYYWFKK-------------SHPVFSKTENTSDL---FPILMDHTYRDCLL 929
            :.:.|:.|||.:|..||:.||             |.....:.:|..|:   ||..::..|.:...
 Worm   807 SGKIKMRYFLSYFHRYYYIKKDAWDQEVAEQQPNSGGETGENQNPLDVQTSFPYEVELAYTELCR 871

  Fly   930 NVRPK----LKIYKSLEQAKAAIDHLQEKLYPQLK----TTNNAQDPSLGTISEISEIDEGGTDE 986
            ..|.|    |:..|:|::|:..:..:::|...:||    ...:.:...|..::.|.|.||...||
 Worm   872 QFRQKKSNSLRWPKNLKEAQDIVTKIEKKFKGELKEFAGEHKDIEGDGLNELNVIEEDDEEDEDE 936

  Fly   987 DSGSSNDQRERQVSGQE-QDQSNDWTENEAEPPLPPLPPPEKSKEDLEFEQLYEKMTTDSY-QER 1049
            :..|..|:.:.:...|: ::.||..|:                :||.||::..::|..:.: |..
 Worm   937 NRDSELDEDDSEEDRQKIENFSNAVTK----------------EEDDEFQRELDRMMGEGFRQSA 985

  Fly  1050 LKEPIKATAKDIPVPMMARLQKKSYDQITGAQTIGPGINNMSQISKSVPNQDGTPGSPGADSPDG 1114
            .:.|:.|...|:.:|  |.::.:....|..|:|:      ..:.:.|...||..      |..|.
 Worm   986 FQMPVAAAQYDVTLP--AAVKDRFNRSIKFAETV------KDEPAASSSKQDND------DDEDV 1036

  Fly  1115 NGKSGGGVGGSDVGAAGAAGASGATSVPFVLMVRGHKGGKQQFKSFVAPSDSHLATNLKRQEQMI 1179
            ..||         ....|:|....|.|  .||||| |..|...|: |...|..|....|  |:..
 Worm  1037 YEKS---------PTIAASGRQKQTRV--TLMVRG-KTNKPSLKT-VNIDDDQLQERWK--EEKA 1086

  Fly  1180 REEKEK--VKRLTLNITERIEEEDYQ---ESLMPPQHRNFTQSYY--QKPNKH 1225
            |||:|:  :|||||....|||.|:.:   .||...:..|..:..:  |.|..|
 Worm  1087 REEQERADMKRLTLGQHRRIEMEEEKALLASLNSSRKGNAKRGQFRPQFPKPH 1139

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Upf2	NP_572434.1	MIF4G	104..300	CDD:214713	72/197 (37%)
		MIF4G	518..701	CDD:214713	56/187 (30%)
		MIF4G	716..934	CDD:280935	67/239 (28%)
		Upf2	980..1192	CDD:281974	56/215 (26%)
smg-3	NP_001368064.1	MIF4G	48..>201	CDD:413423	60/152 (39%)
		MIF4G	461..620	CDD:413423	46/159 (29%)
		MIF4G	650..827	CDD:413423	59/195 (30%)
		Upf2	960..1100	CDD:397938	46/184 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160165777
Domainoid	1	1.000	89	1.000	Domainoid score	I5026
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	405	1.000	Inparanoid score	I1078
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG55567
OrthoDB	1	1.010	-	-		D653534at2759
OrthoFinder	1	1.000	-	-		FOG0005166
OrthoInspector	1	1.000	-	-		oto19047
orthoMCL	1	0.900	-	-		OOG6_103377
Panther	1	1.100	-	-	LDO	PTHR12839
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R816
SonicParanoid	1	1.000	-	-		X3685
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.940