Protein Alignment Nek2 and Nek2

DIOPT Version :9

• Sequence 1: NP_572415.1
• Gene: Nek2 / 31696
• FlyBaseID: FBgn0029970
• Length: 735
• Species: Drosophila melanogaster
• Sequence 2: NP_035022.2
• Gene: Nek2 / 18005
• MGIID: 109359
• Length: 443
• Species: Mus musculus
• Alignment Length: 492
• Identity: 177/492 - (35%)
• Similarity: 258/492 - (52%)
• Gaps: 82/492 - (16%)

  Fly    16 LQDYEVLAVMGNGSFGTCYKVRDKSTGELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQ 80
            ::|||||..:|.||:|.|.|:|.||.|::..||.::|..:.|.:...||||:::||:|:|||||.
Mouse     5 VEDYEVLHSIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEVEKQMLVSEVNLLRELKHPNIVS 69

  Fly    81 YYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNG 145
            ||..:::|...::|||||.|.|||||.::.:....||..||.::.||:.||..||:.||.:...|
Mouse    70 YYDRIIDRTNTTLYIVMEYCEGGDLASVISKGTKDRQYLEEEFVLRVMTQLTLALKECHRRSDGG 134

  Fly   146 -TILHRDIKPANIFLDAAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDV 209
             |:||||:||||:|||:..|.|||||||||:|..|.|||.:|||||:|||||.:....|:.|||:
Mouse   135 HTVLHRDLKPANVFLDSKHNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMSCLSYNEKSDI 199

  Fly   210 WAVGCLVYEMCALRPPFRGRAFDQ--LSEKIAQGEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGI 272
            |::|||:||:|||.|||  .||:|  |:.||.:|.|.|||..||..|.::|..||.:....||.:
Mouse   200 WSLGCLLYELCALMPPF--TAFNQKELAGKIREGRFRRIPYRYSDGLNDLITRMLNLKDYHRPSV 262

  Fly   273 EVIIRHPLVVRNISELDGKFPILVDSGEDFYTLPSGARLFEDEEEDGVHPELSSTMFTEQYSFNE 337
            |.|:..||:...::|                                           ||....|
Mouse   263 EEILESPLIADLVAE-------------------------------------------EQRRNLE 284

  Fly   338 GYGQRRLSVTGVFTPD---LRSELFYSAKRKIFPAKKLQLSDPSLYESIR-REERAEERQVELAE 398
            ..|:|....:.:  ||   :.|||         ..|:.||.|..  :::| ||:..|:::.||..
Mouse   285 RRGRRSGEPSKL--PDSSPVLSEL---------KLKERQLQDRE--QALRAREDILEQKERELCI 336

  Fly   399 ERRRKKDQ----EQQKRDQELLKE-------------APSSPRALTQNIFDEVLKTRLHAIRAQE 446
            ..|..:|:    |...::..||||             .|||......:...|..:....:..::.
Mouse   337 RERLAEDKLARAESLMKNYSLLKEHRLLCLAGGPELDLPSSAMKKKVHFHGESKENTARSENSES 401

  Fly   447 SLLQQKLEELQTREQELQLAEQRVQTLERQMQEKLLQ 483
            .|.:.|..:|:.|....||..|.:..:|:..|.|..|
Mouse   402 YLAKSKCRDLKKRLHAAQLRAQALADIEKNYQLKSRQ 438

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nek2	NP_572415.1	STKc_Nek2	18..281	CDD:270857	133/265 (50%)
		S_TKc	19..281	CDD:214567	132/264 (50%)
Nek2	NP_035022.2	STKc_Nek2	7..271	CDD:270857	134/266 (50%)
		S_TKc	8..271	CDD:214567	133/265 (50%)
		Interaction with PCNT. /evidence=ECO:0000250	264..443		37/199 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	282..303		0/20 (0%)
		Interaction with CEP85. /evidence=ECO:0000250|UniProtKB:P51955	301..443		33/162 (20%)
		Leucine-zipper	306..334		5/27 (19%)
		Necessary for interaction with MAD1L1. /evidence=ECO:0000250	329..443		30/134 (22%)
		Required for microtubule binding and for localization to the centrosomes. /evidence=ECO:0000250	333..370		9/39 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	383..402		5/22 (23%)
		Interaction with SAV1 and STK3/MST2. /evidence=ECO:0000250	402..437		8/47 (17%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	274	1.000	Domainoid score	I1755
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000168
OrthoInspector	1	1.000	-	-		oto95196
orthoMCL	1	0.900	-	-		OOG6_104455
Panther	1	1.100	-	-	LDO	PTHR43671
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R445
SonicParanoid	1	1.000	-	-		X4876
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	10	9.900