DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nonC and PIK3C2A

DIOPT Version :9

Sequence 1:	NP_001284967.1	Gene:	nonC / 31625	FlyBaseID:	FBgn0263968	Length:	3218	Species:	Drosophila melanogaster
Sequence 2:	NP_001308307.1	Gene:	PIK3C2A / 5286	HGNCID:	8971	Length:	1686	Species:	Homo sapiens

Alignment Length:	1561	Identity:	288/1561 - (18%)
Similarity:	479/1561 - (30%)	Gaps:	653/1561 - (41%)

- Green bases have known domain annotations that are detailed below.

  Fly  1099 VELKQQQMTRPEDRELNPFLQRSNVEVNALERLLAKSEESCSSMDALGGVFQQLSLWPSNWDESV 1163
            |..|.:.:::.:..:|:|.   |..:|:.:|.|..:.|::.||:           |....||
Human   258 VSPKSEDISKFDWLDLDPL---SKPKVDNVEVLDHEEEKNVSSL-----------LAKDPWD--- 305

  Fly  1164 SSSGLSERASFSSIHMRQRTEDIVLHKLLEDRCVPDQAKNLLDTQWR------------------ 1210
              :.|.|..|.::.|:.::.....|......|   .|:.|:..||..
Human   306 --AVLLEERSTANCHLERKVNGKSLSVATVTR---SQSLNIRTTQLAKAQGHISQKDPNGTSSLP 365

  Fly  1211 --DSLLNPSFDQRS-----CKELTLLR----HIVQGVSGGQELSLLPVSSGR--C-QNRSKFIS- 1260
              .|||.....|..     |:.:|.|:    :.....:.|..||  ||::.|  | :|.|..:|
Human   366 TGSSLLQEVEVQNEEMAAFCRSITKLKTKFPYTNHRTNPGYLLS--PVTAQRNICGENASVKVSI 428

  Fly  1261 -------------------SAILMRCLAWTQ---------------------LLRQHCAPGS--- 1282
                               ..|:|:.|.|..                     |...||. ||
Human   429 DIEGFQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQNNHCL-GSHEH 492

  Fly  1283 ------WET-----------LCLDAAAAAREEGNLQLAETLLTQFFGQPIGEIAALFSLE---QG 1327
                  |:|           :|.:.|..|.::      ||        |:.....|:.:|   :.
Human   493 IQNCRKWDTEIRLQLLTFSAMCQNLARTAEDD------ET--------PVDLNKHLYQIEKPCKE 543

  Fly  1328 VQTDNP--EMLRGYSELVKCLHLQ-QQQSQTHSGDLSSSIDVCAAL-----------CLNIQKSN 1378
            ..|.:|  |:|..|...|: |.|| :.|.:.....:.:...:|:||           ...::::.
Human   544 AMTRHPVEELLDSYHNQVE-LALQIENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAV 607

  Fly  1379 NQPAAGADLLLNLADWIAVRTCNGLTTNQSPVLIQLLDQLPECPLTCDSSQ-PLAIPQAERMVA- 1441
            |.|.:      ..||  ......|..|::|.....|   .||.|:....:| ..||....|:.|
Human   608 NLPRS------KTAD--VTSLFGGEDTSRSSTRGSL---NPENPVQVSINQLTAAIYDLLRLHAN 661

  Fly  1442 --RLVHSCLQQRPNYAEA-----------LIAYG---NWCYRWGKKVADSCCVLTQADATAISQA 1490
              |....|.|...:..||           ..|:|   ||...:.|...  .|.|:.       ..
Human   662 SGRSPTDCAQSSKSVKEAWTTTEQLQFTIFAAHGISSNWVSNYEKYYL--ICSLSH-------NG 717

  Fly  1491 LDIPQPLESEKL------------DEL-LQALSTEQPPANCV-------------EVCPDAARAR 1529
            .|:.:|::|:|:            ||| :..:...|.|...|             ...||:.:.|
Human   718 KDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGILNQSSGSSPDSNKQR 782

  Fly  1530 DDEAAKNRLRRLTFLADKTPEALDAI---LQIWRRAIANTYDYYKDAARSYFQYLSFKSGSGPEK 1591
                             |.||||..:   |..::|       :.....:..:.:.|..:.|.|..
Human   783 -----------------KGPEALGKVSLPLFDFKR-------FLTCGTKLLYLWTSSHTNSVPGT 823

  Fly  1592 PEGEGVVSQRERLHVD----DSNLVTTTLRLLRLIV----------------------------- 1623
            ...:|.|.:|..|.||    ..:::.||.::.|.|:
Human   824 VTKKGYVMERIVLQVDFPSPAFDIIYTTPQVDRSIIQQHNLETLENDIKGKLLDILHKDSSLGLS 888

  Fly  1624 ---------------KHASGLQEVLEQGLHTTPIAP-WK-VIIPQLFSRLNHHEPYVRKSVCDLL 1671
                           ||.:.|.::|..       || || |.:.:.:|.| |..|
Human   889 KEDKAFLWEKRYYCFKHPNCLPKILAS-------APNWKWVNLAKTYSLL-HQWP---------- 935

  Fly  1672 CRLAKSRPQLVIFPAV----VGANREQQDATAPPATARPTTEDACCYGYLLGELSKQAPEAVQHV 1732
                      .::|.:    :.:....|:..:...|......|        .||:...|:.||.:
Human   936 ----------ALYPLIALELLDSKFADQEVRSLAVTWIEAISD--------DELTDLLPQFVQAL 982

  Fly  1733 K------------LMVKELRRVCLLWDEYW-----IHSLAHIYNTYVSRVSALATDFRPDDHEGK 1780
            |            |:.:.|..:.:..:.||     :|.:.  ::|....|
Human   983 KYEIYLNSSLVQFLLSRALGNIQIAHNLYWLLKDALHDVQ--FSTRYEHV--------------- 1030

  Fly  1781 NNRFNVWRPQLLADLEALVAVTSRPPETTYERSFRKRFDAPIRLTVDALRHRRYPEAWDKLKQLY 1845
                          |.||::|..:                  ||..:.|:.       .||.||.
Human  1031 --------------LGALLSVGGK------------------RLREELLKQ-------TKLVQLL 1056

  Fly  1846 HILQSNMIRGSGSTLKMQSISPVLCGIGRMRISMPGLDAHGPDGDQVYIESVES----SVCVLPT 1906
            ..:...:.:.|||..::     ||                     |..:|.|:|    :.|.||
Human  1057 GGVAEKVRQASGSARQV-----VL---------------------QRSMERVQSFFQKNKCRLP- 1094

  Fly  1907 KTKPKKVA---------FYGSN--------------GQRYTFLFKGMEDLHLDERIMQFLSISNA 1948
             .||..||         |:.||              |:....:||..|||..|...:|.:.|.:.
Human  1095 -LKPSLVAKELNIKSCSFFSSNAVPLKVTMVNADPMGEEINVMFKVGEDLRQDMLALQMIKIMDK 1158

  Fly  1949 IMACRSDAPGNGCYRAHHYSVIPLGPQSGLISWV-DGVTPVFALYKKWQQRRSQVAGNAGAGAVA 2012
            |                               |: :|:.....::|.....|.:       |.|.
Human  1159 I-------------------------------WLKEGLDLRMVIFKCLSTGRDR-------GMVE 1185

  Fly  2013 NVPRRFTDLFYNKLSPLLAKHNMQVS---DPRRQWPISVLLQVLDELSQETPNDLLARELWCQAG 2074
            .||...|      |..:..::.:..|   .|..:|           |.:..|::
Human  1186 LVPASDT------LRKIQVEYGVTGSFKDKPLAEW-----------LRKYNPSE----------- 1222

  Fly  2075 NAAEWRQSVRRFVRCMSVMSMIGYVIGLGDRHLDNVLINLGSGDIVHIDYNV---------CFEK 2130
              .|:.::...|:...:...:..||:|:.|||.||:::. .:|.:.|||:..         .|::
Human  1223 --EEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLR-STGHMFHIDFGKFLGHAQMFGSFKR 1284

  Fly  2131 GRTLRIPEKVPFRLTQNLVQAMGITGIEGP---FRLG---CEYVLKVMRKERETLLTLLEAFVYD 2189
                   ::.||.||.::  |..|.|.|.|   |:|.   |.....::||:....|.||...:..
Human  1285 -------DRAPFVLTSDM--AYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLFLNLLSLMIPS 1340

  Fly  2190 PLVDWTTNDDAQALRRSLN------------AKLQESADGGGA---------------GGLGVGD 2227
            .|.:.|:..|.:.:|.:|.            .:|.||:.|..|               .||...|
Human  1341 GLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSGLPSND 1405

  Fly  2228 ------------------------LKYHKK---DKN---------KGKPLDSDVKR-----QPFL 2251
                                    ..||||   ||:         :|:...|.|.|     |...
Human  1406 EPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGQIEPSFVFRTFDEFQELH 1470

  Fly  2252 SKLGMLQKYW--------------------STNKTELMPQLEEMEQEVGNLQAAQAKQVVAEEEL 2296
            :||.::...|                    :..|.||...|:.:         ..|...|||.:|
Human  1471 NKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSL---------MNASTDVAECDL 1526

  Fly  2297 V 2297
            |
Human  1527 V 1527

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nonC	NP_001284967.1	SMG1	571..1140	CDD:292413	9/40 (23%)
		Rapamycin_bind	1738..1845	CDD:285924	16/111 (14%)
		PIKKc_SMG1	1894..2196	CDD:270714	71/347 (20%)
		FATC	3189..3217	CDD:280430
PIK3C2A	NP_001308307.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..61
		Interaction with clathrin, sufficient to induce clathrin assembly. /evidence=ECO:0000269\|PubMed:16215232	2..142
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	348..367		0/18 (0%)
		PI3K_rbd	409..512	CDD:279173	19/105 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	618..639		5/23 (22%)
		C2A_PI3K_class_II	677..849	CDD:175979	38/204 (19%)
		PI3Ka_II	870..1038	CDD:238441	33/234 (14%)
		PI3Kc_C2_alpha	1043..1395	CDD:270720	91/464 (20%)
		PX_PI3K_C2_alpha	1426..1534	CDD:132822	24/111 (22%)
		Interaction with PtdIns(4,5)P2-containing membranes. /evidence=ECO:0000250\|UniProtKB:Q61194	1488..1493		0/4 (0%)
		C2B_PI3K_class_II	1560..1681	CDD:176027
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:11606566	1608..1619
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.900