Protein Alignment C3G and rasgrf2a

DIOPT Version :9

• Sequence 1: NP_572350.2
• Gene: C3G / 31618
• FlyBaseID: FBgn0259228
• Length: 1571
• Species: Drosophila melanogaster
• Sequence 2: XP_009303357.1
• Gene: rasgrf2a / 558859
• ZFINID: ZDB-GENE-111128-1
• Length: 1274
• Species: Danio rerio
• Alignment Length: 1136
• Identity: 211/1136 - (18%)
• Similarity: 379/1136 - (33%)
• Gaps: 356/1136 - (31%)

  Fly   583 YAQSHNISLNSDLDCSSNISLLNYGVDRLSVRSRSPDENSQC---------------------SF 626
            :.::|..||....:|..|...     |:|   .:..:.|:.|                     :.
Zfish   336 FVRNHQYSLQVLANCKQNRDF-----DKL---LKQYEANAACEGRMLETFLTYPMFQIPRYIITL 392

  Fly   627 DSALNHSREEEDQQQQHQHLRS-FPKLAAMMDEDMD--KMVSYSAAID-----------DKTQTP 677
            ...|.|:..|..:::..:..:| ..:|:.||.:::.  :.:..:.||:           |.:||.
Zfish   393 HELLAHTPHEHVERKSLEFAKSKLEELSKMMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTF 457

  Fly   678 LSTGGGIAGVAGGTGGAGEGVAAAASGD------GETNSNRHSNESGFVSMREFRTSTQTTDYSV 736
            :..|..|.            :.:...|.      |.....:.:....|:..:.|...|:|:...:
Zfish   458 VRQGSLIQ------------LPSVEKGKLSKVRLGSLTLRKEAERQCFLFTKHFLICTRTSGGKL 510

  Fly   737 QSSTKSSSSNSEIAFSISESTAV----GSSSEYQQISQLVSHSQRHI----SSSSSSCTTTTTSS 793
            ....:..:      .|:.|.|.:    .|..:|....|.::|.:..|    :..||........|
Zfish   511 HLLKQGGT------LSLIECTLIEELDTSDEDYNAAGQGLNHLEFKIVVEPADGSSFSVVLLAPS 569

  Fly   794 STTTGYGSSEVEQLQQQ-----------QQQQTTTTPADLAPALPPKSIQRSSLTRHDSPGVGDE 847
            .......:|::.|....           :.....|.|               .:.:.|:....|:
Zfish   570 RQEKAAWTSDISQCIDNIRCNGLMNSVFEDNSKVTVP---------------HMIKSDARLHKDD 619

  Fly   848 LDEVQSSS---------GWASHRSSQSEVAELRQLSPLHHLNHHPHT------------------ 885
            :|...|.:         .:||.......:.:||.|| :..||...||
Zfish   620 VDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLS-IDFLNTFLHTYRIFTTAAVVMDKLADIY 683

  Fly   886 ----ASAGQLQQWHSKHHSLIEG---------PRLQLAGSGSCSAF--------DQRHLDQE--- 926
                .|.....|:||.....|..         .||.|..|.|...|        ...||:.:
Zfish   684 KKPFTSIPVRVQYHSSDRLSITSICPDYSLKITRLALDQSKSLELFFATNQGPWSGEHLNNKSPR 748

  Fly   927 -------PPPLPIKKKHILAYMEICSASTRSIEQHRHTMHACNISRNISHSQTMNIMPMSKELSP 984
                   |||:.|             .|..|...|         ||.:|.|..::....:.:||.
Zfish   749 LSRKFSSPPPISI-------------TSRTSSPIH---------SRKLSLSSPVSCKAGALDLST 791

  Fly   985 ELEMPPALPPKNYKQRKATSMVASP----TLQPVIVTTPPPSPK---PTLGENGSTGRPDSRMAT 1042
            ......:.|...|     ..::.||    |..|:.::..|.||:   .|..|.|...|.|:....
Zfish   792 STFSAASSPTSPY-----NPIICSPPPTCTKAPLDLSRGPSSPELCPSTPEEGGELPRIDAFCGK 851

  Fly  1043 VCEELNDAVASEDAMPE--PRSPVLDSNENVSAVDDGQTFYCHSHQLPAAEME------------ 1093
            :...:..||....::.:  |.:|....:..:|......|.....::.||.:..
Zfish   852 LRRSIRRAVLESVSLDKFIPETPTSSDSGEMSPSRSPSTPRHLRYRQPAGQCPDNSRCAVSPASA 916

  Fly  1094 ---MSEDASSADNQPITTPQVLEEQE---EPTAESRPLVAVHESVKPANVDEDEEAERADMLINM 1152
               .:..|..:..|..|.|:...::|   ...|.:|.|..:...|...:.|.:..||....::.:
Zfish   917 FAIATAAAGHSSPQGFTNPEKSYDKEFLIRRAATNRVLNVLRHWVSKHSQDFEMNAELTTAVMAL 981

  Fly  1153 LEEVNITRYLILKKREEDGPEVKGGYIDALIVHASRVQKVADNAFCEAFITTFRTFIQPIDVIEK 1217
            ||||.....|:.::|                       |.|                  ::::..
Zfish   982 LEEVLRDPDLLPQER-----------------------KAA------------------VNILSA 1005

  Fly  1218 LTHRYTYFFCQVQDNKQKAAKETFALLVRVVNDLTSTDLTSQLLSLLVEFVYQLV-CSGQLYLAK 1281
            |:.       :.||:.|                            |.:|.:.|:| |
Zfish  1006 LSQ-------EEQDDSQ----------------------------LKIEDIVQMVDC-------- 1027

  Fly  1282 LLRNKFVEKVTLYKEPKVYGFVGELGGAGSVGGAGIAGSGGCSGTAGGGNQPSLLDLKSLEIAEQ 1346
                           |:...|.                                 .|.::|:|||
Zfish  1028 ---------------PRAECFE---------------------------------SLSAMELAEQ 1044

  Fly  1347 MTLLDAELFTKIEIPEVL--LFAKDQCEEKSPNLNKFTEHFNKMSYWARSKILRLQDAKEREKHV 1409
            :||||..:|..|...|.|  .:.|....|::|.:.|.::|||.||....|:|:...|...|...:
Zfish  1045 ITLLDHIVFRNIPYEEFLGQGWMKMDKNERTPYIMKTSQHFNDMSNLVASEIMAHADVSSRAGSI 1109

  Fly  1410 NKFIKIMKHLRKMNNYNSYLALLSALDSGPIRRLE--WQKGITEEVRSFC----ALIDSSSSFRA 1468
            :|::.:....|.:||||..|.:.|||:...|.||:  |.| :.::.::..    .::.|...|:.
Zfish  1110 DKWLAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAK-VCKQTKALMDRLQKIVSSEGRFKN 1173

  Fly  1469 YRQALAETNPPCIPYIGLILQDLTFVHVGNQDYLSKGVINFSKRWQQYNIIDNMKRFKKCAYPFR 1533
            .|:.|...||||:||:|:.|.||.|:..|..::..:|::||||.....:||..:::|::..|...
Zfish  1174 LRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQAPYRIE 1238

  Fly  1534 RNERIIRFFDNFKDFMGEEEMWQISEKIKPR 1564
            ...::.:|..:....|.|:.::::|.||:||
Zfish  1239 LQPKVTQFLLDKTLVMDEDTLYELSLKIEPR 1269

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
C3G	NP_572350.2	RasGEFN	1170..1306	CDD:214571	13/136 (10%)
		RasGEF	1335..1558	CDD:238087	69/230 (30%)
rasgrf2a	XP_009303357.1	PH_RasGRF1_2	19..155	CDD:270081
		PH	24..130	CDD:278594
		RhoGEF	244..425	CDD:279015	16/96 (17%)
		PH	478..585	CDD:214574	18/112 (16%)
		PH	489..584	CDD:278594	17/100 (17%)
		RasGEF_N	635..>683	CDD:279012	10/48 (21%)
		REM	<935..1007	CDD:295342	17/112 (15%)
		RasGEF	1033..1265	CDD:238087	71/265 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810