DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Fum1 and FH

DIOPT Version :9

Sequence 1:	NP_572339.1	Gene:	Fum1 / 31605	FlyBaseID:	FBgn0286222	Length:	495	Species:	Drosophila melanogaster
Sequence 2:	NP_000134.2	Gene:	FH / 2271	HGNCID:	3700	Length:	510	Species:	Homo sapiens

Alignment Length:	511	Identity:	369/511 - (72%)
Similarity:	420/511 - (82%)	Gaps:	23/511 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 LPLLQRSTLRGVQQMTKPWAAIGS-----------------LRLASQ-EFRVESDTFGELKVPAD 49
            |.||.||.    ..:..|.||:.|                 .|:||| .||:|.|||||||||.|
Human     5 LRLLARSR----PLVRAPAAALASAPGLGGAAVPSFWPPNAARMASQNSFRIEYDTFGELKVPND 65

  Fly    50 KYYGAQTMRSQINFPIGGATERMPKPVVQAMGILKKAAAEVNKEFGLDSKVSEAISKAADDVISG 114
            |||||||:||.:||.|||.|||||.||::|.||||:||||||:::|||.|::.||.||||:|..|
Human    66 KYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEG 130

  Fly   115 KLYDDHFPLVIWQTGSGTQSNMNVNEVISNRAIELLGGKLGSKTPVHPNDHVNKSQSSNDTFPTA 179
            || :||||||:||||||||:||||||||||||||:|||:||||.|||||||||||||||||||||
Human   131 KL-NDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTA 194

  Fly   180 IHISVALELNNNLKPAIKTLHDALRAKSEEFKDIIKIGRTHTMDAVPLTLGQEFSGYAQQLAYAQ 244
            :||:.|:|::..|.|.::.|||||.|||:||..|||||||||.||||||||||||||.||:.||.
Human   195 MHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAM 259

  Fly   245 ERIDACLPRVYELALGGTAVGTGLNTRKGFAEKCAAKIAELTSLPFVTAPNKFEALAARDAMVEV 309
            .||.|.:||:||||.||||||||||||.|||||.|||:|.||.|||||||||||||||.||:||:
Human   260 TRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVEL 324

  Fly   310 HGVLNTIAVSLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCESLTMLSAQVMG 374
            .|.:||.|.|||||||||||||||||.|||||.|||||||||||||||||||||::||::|||||
Human   325 SGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMG 389

  Fly   375 NQVAVTIGGSNGHFELNVFKPLIVSNVLRSIRLLSDGSRTFTANCVNGIQANRENIAKIMNESLM 439
            |.||||:||||||||||||||:::.|||.|.|||.|.|.:||.|||.|||||.|.|.|:||||||
Human   390 NHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLM 454

  Fly   440 LVTALNPHIGYDKAAKIAKTAHKNGTTLKEEAINLGYLTEQQFNDWVRPEQMLGPK 495
            |||||||||||||||||||||||||:||||.||.|||||.:||::||:|:.|||||
Human   455 LVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK 510

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Fum1	NP_572339.1	fumC	31..495	CDD:234779	356/464 (77%)
FH	NP_000134.2	fumC	49..510	CDD:234779	354/461 (77%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P05042	145..147		1/1 (100%)
		Substrate binding (B site). /evidence=ECO:0000250\|UniProtKB:P05042	176..179		2/2 (100%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P05042	186..188		1/1 (100%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P9WN93	371..373		1/1 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159914
Domainoid	1	1.000	520	1.000	Domainoid score	I305
eggNOG	1	0.900	-	-		E1_COG0114
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H115
Inparanoid	1	1.050	725	1.000	Inparanoid score	I621
Isobase	1	0.950	-	0	Normalized mean entropy	S145
OMA	1	1.010	-	-		QHG61095
OrthoDB	1	1.010	-	-		D1022919at2759
OrthoFinder	1	1.000	-	-		FOG0002459
OrthoInspector	1	1.000	-	-		otm40526
orthoMCL	1	0.900	-	-		OOG6_101016
Panther	1	1.100	-	-	LDO	PTHR11444
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R461
SonicParanoid	1	1.000	-	-		X1641
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	17	16.750