Protein Alignment CG34417 and MICALL2

DIOPT Version :10

• Sequence 1: NP_001259282.1
• Gene: CG34417 / 31591
• FlyBaseID: FBgn0085446
• Length: 5182
• Species: Drosophila melanogaster
• Sequence 2: NP_891554.1
• Gene: MICALL2 / 79778
• HGNCID: 29672
• Length: 904
• Species: Homo sapiens
• Alignment Length: 1053
• Identity: 206/1053 - (19%)
• Similarity: 329/1053 - (31%)
• Gaps: 370/1053 - (35%)

  Fly   452 SSVKKSSRRFSTETTSETVEEYDDRDQPTSR--VVQKAPTPSYAPPSHSPRQSPA--------KD 506
            :.||::|.              |..::|:.:  .||.|..||.| |:..|..|||        ::
Human   116 AGVKRASE--------------DSEEEPSGKKAPVQAAKLPSPA-PARKPPLSPAQTNPVVQRRN 165

  Fly   507 FSTHGFPSVRPNKATQEYPSQRPGIAGEEVVVVRSEKSRQVKQQTSSQRTIETEVVGDDYQEPQR 571
            ....|.|............|...|:.|:.|.:|:...:.......|..|..:....
Human   166 EGAGGPPPKTDQALAGSLVSSTCGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSCT--------- 221

  Fly   572 SPQKLREAPTPSWEQPATRRQPVEEDFSTHGFPSVRTTT---TSTRPDQPD--GEVLHTSKTVSR 631
                |......:..:|.|       ...|...|:..:.:   |...|.||.  |....||.:..:
Human   222 ----LHSGAYKATGEPGT-------FVCTSHLPAAASASPKLTGLVPRQPGAMGVDSRTSCSPQK 275

  Fly   632 NQSANRKTNTERIIETQVEHPN----APSHSTPRSGSPRRSQPRDDYASS--PRGPAGKPSQSR- 689
            .|.||:            ..|:    |..:|..|:..|....|....|:|  .|.|| :||:||
Human   276 AQEANK------------ARPSAWEPAAGNSPARASVPAAPNPAATSATSVHVRSPA-RPSESRL 327

  Fly   690 -PSNTGGSTTTKTTTTSEPLTRRQLQKEREVDAAHRAFAASLRSSSPADSTTSVGSHHQTPRSSV 753
             |:.|.|....:.|.:|                       .:..||.|..|.:..||...|.|
Human   328 APTPTEGKVRPRVTNSS-----------------------PMGWSSAAPCTAAAASHPAVPPS-- 367

  Fly   754 SSNRTFRREMREGSHDSQAPSESSRISSTTVTRHTTGGNVTSNTIKTKTTKPVKTASEPRSPTPK 818
                                                                   |.:||..||:
Human   368 -------------------------------------------------------APDPRPATPQ 377

  Fly   819 AGGS--VTTTTTTITTKSSPSPAPASPTTAAPPTSAPASSAPPDNKLSQYTYTTTKPGDIFSLPP 881
            .||:  |....||:::.|:      |..|..||...|::|.....:...:..:...||
Human   378 GGGAPRVAAPQTTLSSSST------SAATVDPPAWTPSASRTQQARNKFFQTSAVPPG------- 429

  Fly   882 TTPPTINNEPTLTTTRNTTTTTTTTTTATSDNLQNHPKK--SAIE-----------PATDT--DS 931
                      |..:.|..|.:...:..::.:..:|..|:  ||:|           |||..  .|
Human   430 ----------TSLSGRGPTPSLVLSKDSSKEQARNFLKQALSALEEAGAPAPGRPSPATAAVPSS 484

  Fly   932 QPIRKVKLSANEAKVVEEAPCVRRQYYQLGNEGENPETPESSGTPANKKPHQMRRPHDEPEPQLR 996
            ||..:...::..||.::.:                  :|...|.|:..:|         |.|...
Human   485 QPKTEAPQASPLAKPLQSS------------------SPRVLGLPSRMEP---------PAPLST 522

  Fly   997 RSSKSPSVEPRQVQRETTFEGRRVSQDREISIDELILIEETSGA--PGSPKIPSPRAQSPGKPAT 1059
            .|:...|..|.        .|||             .:.|:||.  .|:...|.|.|     |..
Human   523 SSTSQASALPP--------AGRR-------------NLAESSGVGRVGAGSRPKPEA-----PMA 561

  Fly  1060 RSQSPEKPQPRATPRQSPEKEQPAFKQTHEVYTPSQPEKQFPRARSPEKTPGWTQPQVSP--RQS 1122
            :.:|....|..:|..|..:::.||                           || :..:.|  |:|
Human   562 KGKSTTLTQDMSTSLQEGQEDGPA---------------------------GW-RANLKPVDRRS 598

  Fly  1123 P-EKQL----------PRA-QSPEKVPAVRQPSVSPRQSPEKQIPDPKTRDQGPGLPRISPRQSP 1175
            | |:.|          ||| ::|.||......||....:|.:....|:....||.||..||  ||
Human   599 PAERTLKPKEPRALAEPRAGEAPRKVSGSFAGSVHITLTPVRPDRTPRPASPGPSLPARSP--SP 661

  Fly  1176 EKQLPKDVPQKSRQSPEKDLTNQQRREEEIFRSTITTTQKRTTNNLNEEFITNERDNQNQPISEK 1240
            .::....||      ...|:.:...|.|       ...|:....:..||              ||
Human   662 PRRRRLAVP------ASLDVCDNWLRPE-------PPGQEARVQSWKEE--------------EK 699

  Fly  1241 KPQI---------PANAEPNTKPSETIESPDGGFPSKTTEVEAQPEVK------ESPTYRKKGLT 1290
            ||.:         |||..  ..|.||:.||....|...:..|.|.:::      ::...|...|.
Human   700 KPHLQGKPGRPLSPANVP--ALPGETVTSPVRLHPDYLSPEEIQRQLQDIERRLDALELRGVELE 762

  Fly  1291 RR------ETFEDRC----------RQILGMEEDGDTQGTYTERPNNEQEDVNVSHTTIETIQVK 1339
            :|      :..||..          :|:|..:|......:..:|...:|.|:   ...:..:..|
Human   763 KRLRAAEGDDAEDSLMVDWFWLIHEKQLLLRQESELMYKSKAQRLEEQQLDI---EGELRRLMAK 824

  Fly  1340 IEDCPNDDEDDKPRRVTETYVVRTQPKIKVEEELFVD-VTEAEDVEILVNPSKK----------- 1392
            .|...:..|..:.:.:.|.||.....:..:.:.|..| :.|.|:.::|.:..:|
Human   825 PEALKSLQERRREQELLEQYVSTVNDRSDIVDSLDEDRLREQEEDQMLRDMIEKLGLQRKKSKFR 889

  Fly  1393 -----SPKEEDSP 1400
                 |||.:.||
Human   890 LSKIWSPKSKSSP 902

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG34417	NP_001259282.1	PHA03247	<566..1191	CDD:223021	132/670 (20%)
		PTZ00449	<1035..1421	CDD:185628	91/430 (21%)
		PTZ00449	<1394..1702	CDD:185628	4/7 (57%)
		PTZ00121	<2080..2248	CDD:173412
		Smoothelin	2594..2636	CDD:463614
		Smoothelin	3127..3176	CDD:463614
		Smc	<3810..>3916	CDD:440809
		PHA03247	<4126..4601	CDD:223021
		CH_SMTN-like	5074..5179	CDD:409049
MICALL2	NP_891554.1	Forms an intramolecular interaction with the C-terminal coiled coil domain keeping the protein in a closed conformation. /evidence=ECO:0000250	1..260		32/178 (18%)
		CH_MICALL2	2..107	CDD:409102
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	117..178		18/75 (24%)
		LIM_Mical_like_1	188..241	CDD:188828	10/72 (14%)
		PHA03247	<243..649	CDD:223021	118/602 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	251..722		141/698 (20%)
		Mediates targeting to the cell plasma membrane. /evidence=ECO:0000250	261..697		127/647 (20%)
		Necessary and sufficient for interaction with actinins. /evidence=ECO:0000250	261..388		42/219 (19%)
		Forms an intramolecular interaction with the N-terminal Calponin-homology and LIM zinc-binding domains-containing region keeping the protein in a closed conformation. /evidence=ECO:0000250	698..807		23/110 (21%)
		bMERB_dom	743..872	CDD:463467	21/131 (16%)
		Mediates interaction with RAB13 and is required for transition from the closed to the opened conformation. /evidence=ECO:0000250	807..903		19/99 (19%)