DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CG34417 and Micall2

DIOPT Version :9

Sequence 1:	NP_001259282.1	Gene:	CG34417 / 31591	FlyBaseID:	FBgn0085446	Length:	5182	Species:	Drosophila melanogaster
Sequence 2:	NP_777275.2	Gene:	Micall2 / 231830	MGIID:	2444818	Length:	1009	Species:	Mus musculus

Alignment Length:	1020	Identity:	213/1020 - (20%)
Similarity:	340/1020 - (33%)	Gaps:	326/1020 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   466 TSETVEEYDDRDQPTSRVVQKAPTPSYAPPSHSPRQSPAKDFSTHGFPSVRPNKATQEYPSQR-- 528
            :|::.||...:    ..:.|.|..||.|....|| .|||:   |:  |.|:.|:...:.||.:
Mouse   122 SSDSTEELSGK----KGLSQPAKLPSPAQTQRSP-LSPAR---TN--PVVQRNEGGSQRPSPKAA 176

  Fly   529 PGIAGEEVVVVRSEKSRQV--------------------KQQTSSQRTIETEVVGDDYQEP---- 569
            ||.||..|..:.....:.|                    ||.:|:..:......|    ||
Mouse   177 PGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATG----EPGVFV 237

  Fly   570 --QRSPQKLREAPTPSWEQPATRRQPVEEDFSTHGFPSVRTTTTSTRP-------DQPDGEVLHT 625
              ..|.:....:|..|   ....|:|             ...|..|||       .:.:||....
Mouse   238 CTHHSSEVTSVSPKSS---NLASRKP-------------GGVTADTRPFGVSWTVQEANGEGTPL 286

  Fly   626 SKTVSRNQSANRKTNTERIIETQVEHPNAPSHSTPRSGSPRRSQPRDDYASSPRGPAGKPSQSRP 690
            ....:..:.|...|..:..::|:::   .||.|....||          ::.|:.|.
Mouse   287 RVRTAAWEHAGGNTTAKGFVQTELK---PPSTSQVHVGS----------SAGPKLPT-------- 330

  Fly   691 SNTGGSTTTKTTTTSEPLTRRQLQKEREVDAAHRAFAASLRSSSPADSTTSVGSHHQTPRSSVSS 755
                 .|.|.|:.||:.||             |...::.:..||||.|  |..:.:..|..|.|:
Mouse   331 -----ITVTTTSVTSKALT-------------HVTNSSPIGWSSPAQS--SPANFNSRPVVSPSA 375

  Fly   756 NRTFRREMREGSHDSQAP-------SESSRISSTTVTRHTTGGNVTSNTIKTKTTKPVKTASEPR 813
            ..|.    ..||....|.       :.:|..|:|.||...     ||:..||:..:.....:.|.
Mouse   376 RNTH----LPGSQGQTASKGVKTQLNLNSESSNTAVTPAW-----TSSASKTQQAREKFFQTPPS 431

  Fly   814 SPTPKAGGSVTTTTTTITTKSSPSPAPAS--PTTAAPPTS-------APASSAPPDNKLSQYTYT 869
            :|.|               .|:|:|||.|  ||....|||       ||.|..|     :..|..
Mouse   432 APAP---------------ASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVP-----TVVTVP 476

  Fly   870 TTKPGDIFSLPPTTPPTINNEPT--LTTTRNTTTTTTTTTTATSDNLQNHP--KKSAIEPATD-- 928
            |:|...:.|.|.:..||:.:.||  :.|..|:|.:..||..       |.|  |...:..||:
Mouse   477 TSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVV-------NAPTSKVPTVVSATNGR 534

  Fly   929 --------TDSQPIRKVKLSANEAKVVEEAPCVRRQYYQL--------------GNEGENPETPE 971
                    |...| ..:..||::...|.:||.......|.              |.:..|...|.
Mouse   535 VPTVVTAHTGRVP-AVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPA 598

  Fly   972 SSGT----PANKKPHQMRRPHDE--------P------EPQ-----------LRRSSKSPSVEPR 1007
            :|..    |.|:.|.::  |.|:        |      ||.           |:...||||:.||
Mouse   599 TSSVLVTLPKNEVPQKV--PSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPR 661

  Fly  1008 QVQRETTFEGRRVSQDREISIDELILIEETSGAPGSPKIPSPRAQSPGKPATRSQSPE------K 1066
            ..:   |..|.|..             .|.:|..|    |.|.:|.     .:.:.||      |
Mouse   662 VGK---TSVGSRPQ-------------AEVAGVKG----PGPISQE-----GQEEGPEGWRARLK 701

  Fly  1067 PQPRATPR-QSPEKEQPAFKQTHEVYTPSQPEKQFPRARSPEKTPGWTQPQVSPRQSPEKQLPRA 1130
            |..:.||. :|.|:::|...:      |...:.....:.|.:.:...|...:..::.|   .|..
Mouse   702 PVDKKTPAGRSLEQKEPVLAE------PRIGDTSRKASSSSDSSVHITLTSIQHKRKP---CPAG 757

  Fly  1131 QSPEKVPAVRQPSVSPRQS----PEKQI------PDPKTRDQG---------------------- 1163
            ..|.  ||...||.|.|:.    |...:      |:||.::.|
Mouse   758 SGPS--PAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLD 820

  Fly  1164 -----PGLPRISPRQSPEKQLPKDVPQKSRQSPEKDLTNQQRREEEIFRSTITTTQKRTTNNLNE 1223
                 ||....||.:.....:|::..|:..|..|..|...:.|..|:.:.........:.::|..
Mouse   821 SGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMV 885

  Fly  1224 EFITNERDNQNQPISEKKPQIPANAEPNTK-PSETIESPDGGFPSKTTEVEAQPEVKESPTYRKK 1287
            ::.        :.|.||:..:...:|...| ..:.:|........:...:..:||..:||..|::
Mouse   886 DWF--------RLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQR 942

  Fly  1288 GLTRRE-------TFEDRCRQILGMEEDGDTQGTYTERPNNEQED 1325
               .:|       |..||...:..::||         |...::||
Mouse   943 ---EQELLSQYVNTVNDRSDIVDFLDED---------RLREQEED 975

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG34417	NP_001259282.1	Smoothelin	2594..2636	CDD:289290
		Smoothelin	3127..3176	CDD:289290
		GBP_C	<3810..3882	CDD:303769
		coiled coil	3857..3868	CDD:293879
		CH	5076..5179	CDD:237981
Micall2	NP_777275.2	Forms an intramolecular interaction with the C-terminal coiled coil domain keeping the protein in a closed conformation	1..260		35/154 (23%)
		CH	5..102	CDD:278723
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	114..180		21/67 (31%)
		LIM_Mical_like_1	188..241	CDD:188828	7/56 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	247..268		5/36 (14%)
		Mediates targeting to the cell plasma membrane	261..805		145/659 (22%)
		Necessary and sufficient for interaction with actinins	261..393		37/176 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	348..447		31/124 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	655..834		43/214 (20%)
		Forms an intramolecular interaction with the N-terminal Calponin-homology and LIM zinc-binding domains-containing region keeping the protein in a closed conformation	806..913		17/114 (15%)
		DUF3585	842..978	CDD:288945	28/154 (18%)
		Mediates interaction with RAB13 and is required for transition from the closed to the open conformation	913..1009		15/75 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	1	1.000