DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1T and cacna1ia

DIOPT Version :9

Sequence 1:	NP_001259267.1	Gene:	Ca-alpha1T / 31550	FlyBaseID:	FBgn0264386	Length:	3218	Species:	Drosophila melanogaster
Sequence 2:	XP_009297749.2	Gene:	cacna1ia / 566351	ZFINID:	ZDB-GENE-060503-324	Length:	2122	Species:	Danio rerio

Alignment Length:	3036	Identity:	1007/3036 - (33%)
Similarity:	1330/3036 - (43%)	Gaps:	1109/3036 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   292 NWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKM 356
            |.|.:    .||||:||:||||||:||||||||.:..|.:.||::||.||..|:.||||||.:||
Zfish     6 NSCFL----TWFERISIMVILLNCVTLGMYQPCENIDCTSERCQVLQAFDAFIYIFFALEMVVKM 66

  Fly   357 VAMGICGKNTYLADSWNRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILV 421
            ||:||.|:..||.|:|||||||||:||::||.:.::|:|.:||||:||||||:||||:|||||||
Zfish    67 VALGIFGRRCYLGDTWNRLDFFIVMAGMVEYSLDLQNINFSAIRTVRVLRPLKAINRVPSMRILV 131

  Fly   422 MLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWEGILRQRC----SLMRTEGMVYPLTVPEVYRVR 482
            .|||||||||||||||||||||||||||||||.|:||.||    :...|.|:..|
Zfish   132 NLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTSGLTLP---------- 186

  Fly   483 VRVNSLSQYYEFSKDQD--YICSTPNDSGMHLCGNFPPYRIGSLVC---NEEA-----------K 531
                  :.||:..:|.:  :|||...|:|:..|.:.|..|.|...|   .|:|           .
Zfish   187 ------TPYYQPEEDDERPFICSLAQDNGIMSCSDVPARREGGRECCLDKEDALHRQALGLSAEP 245

  Fly   532 LFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDA 596
            |.:.:......|||||||||.|.....||.:|.|:|||||.||:.||.||:||||.:|||||.||
Zfish   246 LVNGSASAMGLCVNWNQYYTRCHTGHTNPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDA 310

  Fly   597 HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERARYTSTSTLASSTNNSEP 661
            |||:::|||:.||:||||||||||||||||||||||:||.:.|:::||||.|:|||||.   :||
Zfish   311 HSFYNFIYFIFLIIIGSFFMINLCLVVIATQFSETKQREHQLMQEQRARYLSSSTLASL---AEP 372

  Fly   662 ATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEGLLPNADNLTFSPSRIKCHHPKCPKYS 726
            ..||.|:.:.:.|:.|:.:||....|.:.:        |..|                   |...
Zfish   373 GDCYEELFQLVCHILRKARRRSAALYYMLR--------GKAP-------------------PPGG 410

  Fly   727 NRKPSSIQDQMITVMVPLNSASNNINNNNNNNSNSSNHNGSGNHTAAGGGNNNNNNNNNNAASCT 791
            .|                                   ..|.|...:.|||.|.|....::..|..
Zfish   411 GR-----------------------------------GRGKGGAGSIGGGANVNGGEKHHCHSAQ 440

  Fly   792 TVALVNGINGSAASVTMSSAHHQQHQLLQHQQQQHQNQQQQQQQQQHSSSDNTEQSLAPDGLPRS 856
            |                   .|..|                :.:..|||.:....|::|:
Zfish   441 T-------------------SHCIH----------------ETKLDHSSENAVSLSISPN----- 465

  Fly   857 SSLKKSTAHHQLKPEGSAAEQKTILLKFPQQMIDSEQLILQLGNLGKSHPCTSGFLSPPTSASRR 921
                         ||
Zfish   466 -------------PE-------------------------------------------------- 467

  Fly   922 PSVMFNEYVLLHTPPALNADPATAGTTTTVAPTVATVAGTAAAAAAAAATGSGSGNNNHQNGGAS 986

Zfish   468 ----------------------------------------------------------------- 467

  Fly   987 GGAGTGGTTEKSNIFSTEKMTQAGDGSIWQVNLPQTIGTIANPYADCSELGIHDAMTCQELLAFS 1051
                                                         :|
Zfish   468 ---------------------------------------------EC------------------ 469

  Fly  1052 VAFSAALPTGQSTLESFYTSLARCDPHTAEALRAHHKPRSVPTGQNQTTPGAEGATVMVASTAGD 1116
                                     |..|.||       |:..|:..|
Zfish   470 -------------------------PQCAAAL-------SLKEGEEST----------------- 485

  Fly  1117 TGQTLQPSTVSAVVGGTIDSHAANHRRKEHHQQSHHHHNNNNTTSHSRNYRSRQGQGNSRMREPR 1181
                               .|:||...:|...:......|       |..:.|....:..:::.|
Zfish   486 -------------------GHSANGEEEEGALEETDRDEN-------RLDKKRSSDSDDTLKKKR 524

  Fly  1182 APTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYF 1246
            ...|.       |.|::       ||                   .||..|      .:||.|||
Zfish   525 TCLGK-------CKDVW-------DE-------------------IRVKLW------GIVESKYF 550

  Fly  1247 QQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVF 1311
            .:||::||||||:|||||:||||.|||.::|..|:||:.:|.:||:||:.|.|.|.|:.|.:|:|
Zfish   551 NRGIMIAILINTISMGIEHHNQPDELTNVLEICNIVFTSMFTLEMILKLTAFGFFEYLRNPYNIF 615

  Fly  1312 DGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAV 1376
            ||||||:|..||    :|...|    ||||||||||||::|||||||.|||||.|:::||||||.
Zfish   616 DGIIVIISVCEI----IGQSDG----GLSVLRTFRLLRVIKLVRFMPALRRQLVVLMKTMDNVAT 672

  Fly  1377 FFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLEREC--TCPEIISRHPQCECDRKHFNNILWAT 1439
            |..||:|||||||||||::||   |||    .|:.|.  |.|           |||:|:::|||.
Zfish   673 FCMLLMLFIFIFSILGMHIFG---CKF----SLKTEAGDTVP-----------DRKNFDSLLWAI 719

  Fly  1440 VTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERRERE 1504
            |||||||||||||:||:|||..||..||||||||||||||||||||||||||||.:|.:..|
Zfish   720 VTVFQILTQEDWNMVLYNGMASTSPLAALYFVALMTFGNYVLFNLLVAILVEGFQAEGDANR--- 781

  Fly  1505 QRELVKKLREETLAENYSDGMYDESRSEADSSTTNDSYYEVRNRWRSAEDVRKLQDSVELIIEAK 1569
                           :|||.         |.|:.|            .|:..| :||::|
Zfish   782 ---------------SYSDD---------DRSSCN------------LEETEK-KDSLQL----- 804

  Fly  1570 SNMHRQRLLQPTHDYQINELPAS-----SAAPSASGTSGASAPGDRERDRDRDRDRERDRDRDRE 1629
                        .|.:|:.|..:     :.||:|.|    ..|.:|
Zfish   805 ------------SDPKISTLTPNGHLDLAPAPNARG----FYPSER------------------- 834

  Fly  1630 RDREAGGGGKEEGAQHAKPRGGLKKTYSIKERRSEAPRLSKIRLARDPPIITTTAATPQDSPSTT 1694
                                    .::::..|:|....|.:..|.:                 ||
Zfish   835 ------------------------LSFALGSRKSSVSSLGRASLEQ-----------------TT 858

  Fly  1695 LEPGMS--FRQWGDMEPPSPPSPSLLRP--PNIFTGGQRSLDEGIPSIDLIPPSPVLSHKPLNIL 1755
            |.||.:  :..||             ||  |.|::  :||                    ..|.|
Zfish   859 LCPGRASLYHNWG-------------RPARPGIWS--RRS--------------------SWNSL 888

  Fly  1756 NASQLGVGMGGGVPSTAGSSSSMHSVIIDDISKSSSSTAAATPIYVPTISSTADAQSQSHSLNDV 1820
            ..|...:|||||                       .|....:|              ..|.....
Zfish   889 GRSSRCLGMGGG-----------------------GSLRVRSP--------------HCHPAEQE 916

  Fly  1821 SVGSSPGGEIPATGMSRNANTGASTSGSSSSERLPLAPPPQQGSFKQRLRRGSSKKRRASALALA 1885
            |:.|.|    |.                      ||.||.....|..|      ::|||.:|.|.
Zfish   917 SLLSPP----PP----------------------PLHPPLLPRHFPPR------RERRALSLELP 949

  Fly  1886 TDDNPARRTLDNQARRTQDEEEEQQQLNNGGDNSCLLRNSNAVVSGSSSGTKETNRLSPQNSIRR 1950
            .                                  ||:     |.|..        |.|.:. |:
Zfish   950 E----------------------------------LLQ-----VPGPP--------LPPLHP-RQ 966

  Fly  1951 LSNTLSIGSGPVGSRRASACIFNSQVYQNLN-QPPKLRPGSGQRRMSSIELAFSKTSHLNLHNLE 2014
            ...:.|.|.|.||.            :|:.| :.|.::|
Zfish   967 RKKSFSGGLGSVGE------------HQDCNGKTPSVQP-------------------------- 993

  Fly  2015 ANRKSLSYTNSKMDLDKWNKSYGNLNEPDNMLQQYMEARDKRKNSISHYNLKKRLEEKELQQLQQ 2079
                                                                        |.:.:
Zfish   994 ------------------------------------------------------------QIINE 998

  Fly  2080 LHQQQLLQQRQDSFSSTTQQQQQQLQQHRLSKDQQQLAMQPHSMVPGGGERYS---KLKMLIEQL 2141
            ::.|           ..|::.::.|:      |:..               ||   :::.::|..
Zfish   999 VYPQ-----------VNTRKDREDLE------DELD---------------YSLCFRIQKMMEVY 1031

  Fly  2142 TPKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTER 2206
            .|....| |||:|:::|...|:||.:|...:..|.||.|||.||..||||:|:|||.|...|.||
Zfish  1032 RPDWCET-REDWSVFLFSPQNKFRLLCQSIIAHKLFDYVVLAFIFSNCITVALERPKILQGSLER 1095

  Fly  2207 LFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPR 2271
            |||..:||:||.:|..||.:|||:.|::.|..||..|.|||:||.||.:|:||:::|:  ....:
Zfish  1096 LFLTVSNYIFTAIFVGEMTLKVVSMGLYIGEQAYLRSSWNILDGFLVFVSLIDIVVSM--AGGAK 1158

  Fly  2272 IFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGT 2336
            |.|:|||.||||:|||||||:|||||||||:||::||:||||||||||.|||||||||||||||.
Zfish  1159 ILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLITSLKPIGNIVLICCAFFIIFGILGVQLFKGK 1223

  Fly  2337 FYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGV 2401
            ||||.|.::|.:.|..:| .:....|.:.|||||:||:||||||||:|:||||||||.|||||.|
Zfish  1224 FYYCLGLDVKNITNKSDC-LLANYKWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYHGLDAVAV 1287

  Fly  2402 DQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKK 2466
            |||||.|.|.|.|||||:|:|:|.||||||||||||||||:||:.||.||..||..||..:||||
Zfish  1288 DQQPITNNNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEVEEAKRREEKRQRRMEKK 1352

  Fly  2467 RRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYF 2531
            ||:..:.|||.:||..|:.:|.:.||.|.|:.|..:|.:|||||::|:|..|..|:.|||..|||
Zfish  1353 RRKAQKLPYYASYSHVRLMIHTLCTSHYLDIFITFIICVNVVTMSLEHYSQPHSLEIALKYCNYF 1417

  Fly  2532 FTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMR 2596
            ||:.|:|||.:||:|.|::.:.||||||||:.|||||::||.|||:|.:.  .:||||||||:||
Zfish  1418 FTSTFVLEAVLKLIAFGFRRFFKDRWNQLDLAIVLLSVMGITLEEIEISA--ALPINPTIIRIMR 1480

  Fly  2597 VLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQ 2661
            ||||||||||||||.|:|||||||:|||||||||||||.|||||:|||||||||.|.|:::.||:
Zfish  1481 VLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGELVCNEDYPCE 1545

  Fly  2662 GLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDNCDDAADCVRNCCVSSV--IAPIFFVIF 2724
            |:..||.|.||||||||||:|:||||||||||||||: |...    ...|..|:  |:|::||.|
Zfish  1546 GMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRE-CPPG----EYTCNPSLQFISPLYFVSF 1605

  Fly  2725 VLMAQFVLVNVVVAVLMKHLEESHKQMEDELDMEVELERELVREQEFAQEQKLC----QQLAE-- 2783
            ||.|||||:|||||||||||::|:|:.::|.:|:.|:|.||      ||....|    ..:|:
Zfish  1606 VLTAQFVLINVVVAVLMKHLDDSNKEAQEEAEMDAEIEMEL------AQGTLCCIGGGVAVADRT 1664

  Fly  2784 ------AQSKAAAPPR-PLAKVKSLPKNFI---YSTPSLDKKFPAVSGVNNPSLTSS--AATNLN 2836
                  |.|.:..||. |:....:...|.|   ||....:....:||.:...|....  ...||:
Zfish  1665 GMGHQGAASCSVVPPHSPITHPVAHEPNSIRRLYSPAQENLWLDSVSLLIKDSFEGEMLMIDNLS 1729

  Fly  2837 QVATAGSGGAAPGAVPAGSGAGPRRQTVQYFQQP---------PQSMLGGLSLAEM-GGTLTPQA 2891
                        |:|            ..::..|         ||..   :.:||: ..:|..:.
Zfish  1730 ------------GSV------------FHHYSSPPVCKDCRTHPQEQ---IHMAELEQASLRSEQ 1767

  Fly  2892 LGARLGEPFGGGGGNKSGPRRQSYWQINPIRKRGVLSKERSLDEQAI-RRRNLEAKRTSCDSL-- 2953
            |..:...|                          .|..:.|||||:: :....|.|...||..
Zfish  1768 LSDKSSSP--------------------------ALPDDLSLDEQSVYQMAAKEGKERVCDECHH 1806

  Fly  2954 ---PWG-GDALDCRRGTIFESLESDGG-------------GVGG---GGSGGGGGGLGGGVSYDL 2998
               |.| |::...|...:..|...|||             .:||   ..|.....|:.||.|
Zfish  1807 SEEPGGRGNSRLRRSSRVHSSGTEDGGTCQSPHHNTPARHSIGGVPCSSSSQEHPGVSGGSS--- 1868

  Fly  2999 RSVRSADVGHSEMDGDVSLSVVSALVPSVTTPLPPPLSLPIVTPTSTPTPLQLPMPMPMPMAHPP 3063
                                                         .:.||:.||.....|.|...
Zfish  1869 ---------------------------------------------RSNTPVCLPAEFFHPAAAAL 1888

  Fly  3064 PRRPFGWSQSVDQGCLRSNLLLSVPRSMPP-RSRSGSTKQLFKQQALDEDADMDENSLLLPTAAG 3127
            |..|.|.:....:|     |.|:.|.|... ||...:|:.|..|.....|:.:           .
Zfish  1889 PAPPRGRTGHKPRG-----LRLTSPASWASLRSPGANTRLLTTQYPSHSDSSL-----------A 1937

  Fly  3128 GGSGPGSVAVIASSSLDLPDQSGSSKQILPDIALGVSKSDSSDILR------IISERRRMDQREQ 3186
            .||..||:.......|..   ..|:.|.|.|..|....|.|::.||      .|.:..|..||.:
Zfish  1938 TGSSEGSLPTTMEEGLSF---IVSTPQPLLDTLLDERTSLSTETLRPSPPATHILQTTRGHQRSR 1999

  Fly  3187 REPEQD-------QDNEAE--------DRDSDYKELLLVKSPQSSM 3217
            ...|.|       |.:||:        ::..|.::|   ...|||:
Zfish  2000 SSCETDINTGSIRQGSEADVGLVWCSSEQTCDSQQL---SETQSSL 2042

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1T	NP_001259267.1	Ion_trans	332..636	CDD:278921	181/323 (56%)
		Ion_trans	1261..1495	CDD:278921	147/235 (63%)
		Ion_trans	2192..2448	CDD:278921	164/255 (64%)
		Ion_trans	2510..2728	CDD:278921	142/219 (65%)
cacna1ia	XP_009297749.2	Ion_trans	12..350	CDD:306908	202/353 (57%)
		Ion_trans	547..774	CDD:306908	158/252 (63%)
		Ion_trans	1063..1334	CDD:306908	176/273 (64%)
		Ion_trans	1378..1633	CDD:306908	169/261 (65%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	421	1.000	Domainoid score	I612
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1560	1.000	Inparanoid score	I88
OMA	1	1.010	-	-		QHG59758
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0001656
OrthoInspector	1	1.000	-	-		otm24624
orthoMCL	1	0.900	-	-		OOG6_102692
Panther	1	1.100	-	-	O	PTHR10037
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R8566
SonicParanoid	1	1.000	-	-		X1056
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	12.010