DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1T and cacna1ha

DIOPT Version :9

Sequence 1:	NP_001259267.1	Gene:	Ca-alpha1T / 31550	FlyBaseID:	FBgn0264386	Length:	3218	Species:	Drosophila melanogaster
Sequence 2:	XP_021330381.1	Gene:	cacna1ha / 560875	ZFINID:	ZDB-GENE-130103-1	Length:	2198	Species:	Danio rerio

Alignment Length:	2988	Identity:	1040/2988 - (34%)
Similarity:	1369/2988 - (45%)	Gaps:	984/2988 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   308 ILVILLNCITLGMYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSW 372
            :|||||||:||||||||.|..|.:..|.:||.|||.||||||:||.|||:|:||.|.|.||.|:|
Zfish     1 MLVILLNCVTLGMYQPCEDLKCQSEWCIVLQAFDDCIFAFFAVEMVIKMIALGIFGINGYLGDTW 65

  Fly   373 NRLDFFIVLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLL 437
            ||||||||:||::||.:...|.:|:||||:|||||||||||:|||||||.|||||||||||||||
Zfish    66 NRLDFFIVMAGMMEYSLDGHNASLSAIRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLL 130

  Fly   438 CFFVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYPLTVPEVYRVRVRVNSLSQYY--EFSKDQDY 500
            ||||||||||:|||||.|:||.||.:            |...|.:..::.:|.||  |..:|..:
Zfish   131 CFFVFFIFGIVGVQLWAGLLRNRCFM------------PSKVRDQYNLSYMSLYYENEDGEDNPF 183

  Fly   501 ICSTPNDSGMHLCGNFPPYRIGSLVCNEEAKLF-----DFNEPTNTSCVNWNQYYTTCKQSGENP 560
            |||:..|:||..|...||.:.| :.|...|...     ..:...|.|||||||||:.||....||
Zfish   184 ICSSSKDNGMKRCSAVPPLKEG-VECTLNASFLGHVYSGLSGTGNYSCVNWNQYYSECKPGDLNP 247

  Fly   561 FQGTISFDNIGMAWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIA 625
            .:|.::|||||.||:|||.||:||||.||||||.|||||:::|||:|||::||||||||||||||
Zfish   248 HKGAVNFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIA 312

  Fly   626 TQFSETKKREMERMRQERARYTST-STLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRL 689
            |||:|||:||...|:::||||.|. |||||   .|||.:||.|::||::||:|:.|||:   .|:
Zfish   313 TQFAETKQRENMLMKEQRARYRSNDSTLAS---YSEPGSCYEEMLKYVSHLYRKVKRRL---SRI 371

  Fly   690 YKYQRQQRKEGLLPNADNLTFSPSRIKCHHPKCPKYSNRKPSSIQDQMITVMVPLNSASNNINNN 754
            |...:.:|::.:.||
Zfish   372 YNSWQSKRRKKVNPN-------------------------------------------------- 386

  Fly   755 NNNNSNSSNHNGSGNHTAAGGGNNNNNNNNNNAASCTTVALVNGINGSAASVTMSSAHHQQHQLL 819
                            |.||||.|.                                 |.:|:..
Zfish   387 ----------------TGAGGGANG---------------------------------HSRHRGY 402

  Fly   820 QHQQQQHQNQQQQQQQQQHSSSDNTEQSLAPDGLPRSSSLKKSTAHHQLKPEGSAAEQKTILLKF 884
            .|..|...:..||.|:|....|:.:...:|..|...:..::..||..||          |:
Zfish   403 GHWVQSIHSLIQQHQRQHCHLSNGSPSPVATTGSSEALEMRSITAGQQL----------TV---- 453

  Fly   885 PQQMIDSEQLILQLGNLGKSHPCTSGFLSPPTSASRRPSVM---FNEYVLLHTPPALNADPATAG 946
                              .|.|..:..||..|.:..  |:.   |.|.....||
Zfish   454 ------------------SSQPTATHNLSSSTQSVH--SIYQGDFREAPQERTP----------- 487

  Fly   947 TTTTVAPTVATVAGTAAAAAAAAATGSGSGNNNHQNGGA-------------SGGAGTGGTTEKS 998
             |....|.::.:                       |||.             |||...|....||
Zfish   488 -TNVAVPALSRL-----------------------NGGMNYPTILPSLICNYSGGGSLGKERGKS 528

  Fly   999 NIFSTEKMTQAGDGSIWQVNLPQTIGTIANPYADCSELGIHDAMTCQELLAFSVAFSAALPTGQS 1063
            :..:..::.|..|      .|.|.|           |...|..:..|        .:..:|| |.
Zfish   529 DANTGHEVIQDFD------KLHQQI-----------EAHSHSRVLLQ--------MAGVVPT-QL 567

  Fly  1064 TLESFYTSLARCDPHTAEALRAHHKPRSVPTGQNQTTPGAEGATVMVASTAGDTGQTLQPSTVSA 1128
            .||     :..| |:...||.:    ..:..|.:. .|.:||.  ||...:.|.|         .
Zfish   568 LLE-----VMSC-PYCVRALES----MDLEIGDSD-YPRSEGD--MVYEFSYDAG---------F 610

  Fly  1129 VVGGTIDSHAANHRRKEHHQQSHHHHNNNNTTSHSRNYRSRQGQGNSRMREPRAPTGNYMEDYAC 1193
            :.|           |..||                            :|:|.:.....:.||:
Zfish   611 ITG-----------RSAHH----------------------------KMQEKQNRLVQFWEDF-- 634

  Fly  1194 CYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINT 1258
                                                    |..:.|:|:.|||.:||::||||||
Zfish   635 ----------------------------------------RERLTRIVDSKYFNRGIMIAILINT 659

  Fly  1259 LSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEI 1323
            ||||||||.||.|||.|:|.||:||:.:|.:|||.|::|.|.|.||.|.:|:|||:||::|..||
Zfish   660 LSMGIEYHEQPEELTNILEISNIVFTSMFVLEMLFKLLAFGIFGYIRNPYNIFDGVIVVISVWEI 724

  Fly  1324 CQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIF 1388
                :|:..|    ||||||||||||:||||||:|.|||||.|:::||||||.|..||:||||.|
Zfish   725 ----IGHADG----GLSVLRTFRLLRVLKLVRFLPALRRQLLVLMKTMDNVATFCMLLMLFIFTF 781

  Fly  1389 SILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNV 1453
            |||||:|||   |||  ...:|...|.|           |||:|:::|||.||||||||||||||
Zfish   782 SILGMHLFG---CKF--SLKMENGDTIP-----------DRKNFDSLLWAIVTVFQILTQEDWNV 830

  Fly  1454 VLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAILVEGFSSERNERREREQRELVKKLREETLA 1518
            ||:|||..||.|||||||||||||||||||||||||||||.:|.:
Zfish   831 VLYNGMASTSPWAALYFVALMTFGNYVLFNLLVAILVEGFQAEGD-------------------- 875

  Fly  1519 ENYSDGMYDESRSEADSSTTNDSYYEVRNRWRSAEDVRKLQDSVELIIEAKSNMHRQRLLQPTHD 1583
            .|.|||  ||.::..:|.                |.:.:||.|..|.|.           .|
Zfish   876 ANKSDG--DEEKTSVNSE----------------EKMEQLQSSEPLSIS-----------DP--- 908

  Fly  1584 YQINELPASSAAPSASGTSGASAPGDRERDRDRDRDRERDRDRDRERDREAGGGGKEEGAQHAKP 1648
                :|...|...||:|                                            |..|
Zfish   909 ----DLKFYSLMLSANG--------------------------------------------HVDP 925

  Fly  1649 RGGLKKTYSIKERRSEAPRLSKIRLARDPPIITTTAATPQDSPSTTLEPGMSFRQWGDMEPPSPP 1713
            .|.|                       .||||..|||||..:|.::|.|...|
Zfish   926 CGTL-----------------------PPPIIMRTAATPMPTPKSSLGPESVF------------ 955

  Fly  1714 SPSLLRPPNIFTGGQRSLDEGIPSIDLIPPSPVLSHKPLNILNASQLGVGMGGGVPSTAGSSSSM 1778
                                     ||                           ..|..||:.|:
Zfish   956 -------------------------DL---------------------------TESRRGSAVSI 968

  Fly  1779 HSVIIDDISKSSSSTAAATPIYVPTISSTADAQSQSHSLNDVSVGSSPGGEIPATGMSRNANTGA 1843
            .....|..|.||...:...|     ..|.::..|:..|.|  |:|.:|       .:.|.
Zfish   969 DPNAFDQKSLSSPRRSPCHP-----RGSGSNWGSRRSSWN--SLGRAP-------SLKRK----- 1014

  Fly  1844 STSGSSSSERLPLAPPPQQGSFKQRLRRGSSKKRRASALALATDDNPARRTLDNQARRTQDEEEE 1908
            .|||    ||..|.....:|||                                     :|::.|
Zfish  1015 DTSG----ERESLLSGEGRGSF-------------------------------------EDDDIE 1038

  Fly  1909 QQQLNNGGDNSCLLRNSNAVVSGSSSGTKETNRLSPQNSIRRLSNTLSIGSGPVGSRRASACIFN 1973
            .::|.|              :||.|
Zfish  1039 AEKLTN--------------ISGGS---------------------------------------- 1049

  Fly  1974 SQVYQNLNQP-----PKLRPGSGQRRMSSIELAFSKTSHLNLHNLEANRKSLSYTNSKMDLDKWN 2033
                  |..|     |:| |..|:                   .|:.|.:|   .:...||.
Zfish  1050 ------LQHPSSLDIPEL-PQMGE-------------------YLDCNGRS---QHILADLS--- 1082

  Fly  2034 KSYGNLNEPDNMLQQYMEARDKRKNSISHYNLKKRLEEKELQQLQQLHQQQLLQQRQDSFSSTTQ 2098
               .:||:.|::.::.::                                       |||.
Zfish  1083 ---ASLNKEDSIAEEDLD---------------------------------------DSFC---- 1101

  Fly  2099 QQQQQLQQHRLSKDQQQLAMQPHSMVPGGGERYSKLKMLIEQLTPKHFTTEREDYSLYIFPEDNR 2163
                                             .:|:..:....|| :..:.|::|||:|...|:
Zfish  1102 ---------------------------------FRLRRTLAAYKPK-WCKDHEEWSLYLFSPHNK 1132

  Fly  2164 FRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKV 2228
            ||.:|...::.|.||.|||:||.|||||:|:|||:|..|  |||||..:||||||:|..||.:||
Zfish  1133 FRMMCQKLISHKMFDYVVLVFIFLNCITIALERPHIQQS--ERLFLLVSNYVFTVIFVAEMTVKV 1195

  Fly  2229 VATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINR 2293
            ||.|.:.|:.:|..|.||::||.||.:|:||:|:|| :.:..||||||||.||||:|||||||:|
Zfish  1196 VALGFYSGNQSYLKSTWNVLDGVLVFVSLIDILVSL-AWTGNRIFGILRVLRLLRTLRPLRVISR 1259

  Fly  2294 APGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECRRIP 2358
            ||||||||:||::|||||||||||||.|||:|||||||||||.|::|||.:.:.:.|..:|  :.
Zfish  1260 APGLKLVVETLITSLRPIGNIVLICCAFFIVFGILGVQLFKGKFFHCEGGDTRNITNKSDC--LQ 1322

  Fly  2359 GNV-WTNRKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFIL 2422
            .|: |..||||||:||:|||||||||.:||||||||.|||||||||||..|:|.|.|||||:|:|
Zfish  1323 ANLKWIRRKYNFDNLGQALMSLFVLSCKDGWVNIMYDGLDAVGVDQQPERNHNPWMLLYFISFLL 1387

  Fly  2423 LVGFFVLNMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMH-------EPPYYTNYS 2480
            :|.||||||||||||||||:||::||:.|......||...||||||...       ..|||.:||
Zfish  1388 IVSFFVLNMFVGVVVENFHKCRQDQEEVEARLLELKRQKLMEKKRRSKENCGSEALRRPYYADYS 1452

  Fly  2481 PTRMFVHNVVTSKYFDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLV 2545
            |.|:::|.:.|:.|.||.|..:|.:|||||::|::..||.|..|||..||.||.:||:||.:|||
Zfish  1453 PARLYIHTLCTNHYLDLFITGIICINVVTMSIEHFNQPSYLDEALKYCNYVFTIIFIIEALLKLV 1517

  Fly  2546 ALGWKLYLKDRWNQLDVGIVLLSIVGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMA 2610
            |.|.:.:.||||||||:.||||||:||.|||::.|.  .:||||||||:||||||||||||||||
Zfish  1518 AFGIRRFFKDRWNQLDLAIVLLSIMGITLEEIKMNA--ALPINPTIIRIMRVLRIARVLKLLKMA 1580

  Fly  2611 NGIRALLDTVMQALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMA 2675
            .|:|:|||||||||||||||||||.|||||:|||||||||:|||::..||:||..||.|.|||||
Zfish  1581 TGMRSLLDTVMQALPQVGNLGLLFMLLFFIYAALGVELFGKLECTENNPCEGLSPHATFENFGMA 1645

  Fly  2676 FLTLFRVATGDNWNGIMKDTLRDNCDDAADCV--RNCCVSSV--IAPIFFVIFVLMAQFVLVNVV 2736
            |||||||:||||||||||||||       :|:  ...|:|.:  ::||:||.|||||||||||||
Zfish  1646 FLTLFRVSTGDNWNGIMKDTLR-------ECLPSETQCLSYLPWVSPIYFVTFVLMAQFVLVNVV 1703

  Fly  2737 VAVLMKHLEESHKQMEDELDMEVELERELVREQEFAQEQKLCQQLAEAQSKAAAPPRPLAK-VKS 2800
            |||||||||||:|:.:::.:|:..::..:::|         .::|:...:.|.|.|...|. ::.
Zfish  1704 VAVLMKHLEESNKEAKEDAEMDAAIKLAILKE---------TRRLSTISASATAGPEGRATYIEP 1759

  Fly  2801 LPKNFIYSTPSLDK---------------------KFPAVSGVNNPSLTSSAATNLNQVATAGSG 2844
            .|     .:|..|:                     ..|..|.:..|...:||...|.:|.|
Zfish  1760 HP-----DSPEQDEGMQRTQGNLLSPRKMSVSRMHSLPNDSYMFRPVRPASAPYPLEEVET---- 1815

  Fly  2845 GAAPGAVPAGSGAGPRRQTVQYFQQPPQSMLGGLSLAEMGGTLTPQALGARLGEPFGGGGGNKSG 2909
              ....:..||       ......||.:|    |||.::.| :.|.:
Zfish  1816 --CQDYLELGS-------ITSVHSQPCES----LSLLQVPG-VPPHS------------------ 1848

  Fly  2910 PRRQSYWQINPIRK------------RGVLSKERSLDEQAIRRRNLEAKRT-------------- 2948
                   |.|.:.|            ..:|.::|::...:|...|.::|..
Zfish  1849 -------QFNCLPKIPPPMPSPTHSIDRILRRQRAIKNDSIDMHNFDSKENVERQKGITTGSFKL 1906

  Fly  2949 ---SCDSLPWGGDALDCRRGTI--FE-------------SLESDGGGVGGGGSGGGGGGLGGGVS 2995
               ....:..|...|..|..::  |:             ||.|........||           .
Zfish  1907 HVPQITGISQGSPLLPPRGPSVHTFQQPHSQHNISSRPPSLASSSRSTSPSGS-----------M 1960

  Fly  2996 YDLRSVRSADVGHSEMDGDVSLSVVS-----ALVPSVT--TPLPPPLSLPIVTPTSTPTPLQLPM 3053
            :|.......:|.|......:.:.|.:     :|.|..|  |.||.|     |...|:...|.:..
Zfish  1961 FDSADPTDEEVRHINSTARLWIGVHAEARSHSLSPYSTSGTLLPIP-----VKNCSSAANLTIKD 2020

  Fly  3054 P-----------MPMPMAHPPPRR-PFGWSQSVDQGCLRSNLL-----------LSVPRSMPPRS 3095
            |           :..|.|....|| ......|.|.|.....|.           |.:||......
Zfish  2021 PKKCFSVDTEGFLERPYASDDHRRHSIEICSSGDDGLFGQELCEKRHVPTRGKSLHIPRKTKMSP 2085

  Fly  3096 RSGSTKQLFKQQALDEDADMDE--NSLLLPTAAGGGSGPGSVAVIASSSLDLPDQSGSSKQIL 3156
            ...|.:..|::......|.|.:  :|:||....     |.....:...|||||:...|::.::
Zfish  2086 PCISIEPPFEKDVSPSHASMKKLSDSMLLRRRT-----PSYDLALQRDSLDLPENQLSTQPLI 2143

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1T	NP_001259267.1	Ion_trans	332..636	CDD:278921	185/310 (60%)
		Ion_trans	1261..1495	CDD:278921	149/233 (64%)
		Ion_trans	2192..2448	CDD:278921	170/256 (66%)
		Ion_trans	2510..2728	CDD:278921	146/221 (66%)
cacna1ha	XP_021330381.1	Ion_trans	1..323	CDD:306908	202/334 (60%)
		Ion_trans	644..871	CDD:306908	161/250 (64%)
		Ion_trans	1143..1413	CDD:306908	183/274 (67%)
		Ion_trans	1464..1695	CDD:306908	155/239 (65%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	421	1.000	Domainoid score	I612
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1560	1.000	Inparanoid score	I88
OMA	1	1.010	-	-		QHG59758
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0001656
OrthoInspector	1	1.000	-	-		otm24624
orthoMCL	1	0.900	-	-		OOG6_102692
Panther	1	1.100	-	-	LDO	PTHR10037
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1056
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.880