Protein Alignment Ca-alpha1T and Cacna1s

DIOPT Version :10

• Sequence 1: NP_001259267.1
• Gene: Ca-alpha1T / 31550
• FlyBaseID: FBgn0264386
• Length: 3218
• Species: Drosophila melanogaster
• Sequence 2: NP_001074492.1
• Gene: Cacna1s / 12292
• MGIID: 88294
• Length: 1852
• Species: Mus musculus
• Alignment Length: 2581
• Identity: 483/2581 - (18%)
• Similarity: 764/2581 - (29%)
• Gaps: 1269/2581 - (49%)

  Fly   268 EPNLPYPGF---PEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLGMYQPCVDDAC 329
            :|..|.|..   |..::..||.....|..|:.::....||.:.:|.|..||:.|.:|.|..:|.
Mouse    15 QPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDD- 78

  Fly   330 VTNRCKI-LQIFDDIIFAFFALEMTIKMVAMG-ICGKNTYLADSWNRLDFFIVLAGLLEYVMHVE 392
             .|...: |:..:......|::|..:|::|.| :..::.||...||.|||.||..|:...::...
Mouse    79 -NNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQV 142

  Fly   393 N---------------LNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVF 442
            |               |::.|:|..|||||||.::.:||:::::..:...:..|.::.||..|:.
Mouse   143 NIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMV 207

  Fly   443 FIFGIIGVQLWEGILRQRCSLMRTEGMVYPLTVPEVYRVRVRVNSLSQYYEFSKDQDYICSTPND 507
            .|:.|||::|::|.:.:.|..:.|                                |.:.:..|:
Mouse   208 IIYAIIGLELFKGKMHKTCYFIGT--------------------------------DIVATVENE 240

  Fly   508 SGMHLCGNFPPYRIGSLVCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGM 572
            ...      |..|.||            ..|..   :|.::    |:.....|..|...|||.|.
Mouse   241 KPS------PCARTGS------------GRPCT---INGSE----CRGGWPGPNHGITHFDNFGF 280

  Fly   573 AWVAIFLVISLEGWTDIMYYVQDA-HSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREM 636
            :.:.::..||:|||||::|:|.|| .:.|.|||||.||::||||::||.|.|::.:|  ||:||.
Mouse   281 SMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEF--TKEREK 343

  Fly   637 ERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEGL 701
            .:                                         .|...:|.|    ::||.:|.|
Mouse   344 AK-----------------------------------------SRGTFQKLR----EKQQLEEDL 363

  Fly   702 LPNADNLTFSPSRIKCHHPKCPKYSNRKPSSIQDQMITVMVPLNSASNNINNNNNNNSNSSNHNG 766
                                                                             
Mouse   364 ----------------------------------------------------------------- 363

  Fly   767 SGNHTAAGGGNNNNNNNNNNAASCTTVALVNGINGSAASVTMSSAHHQQHQLLQHQQQQHQNQQQ 831
                                             .|..:.:|..
Mouse   364 ---------------------------------RGYMSWITQG---------------------- 373

  Fly   832 QQQQQQHSSSDNTEQSLAPDGLPRSSSLKKSTAHHQLKPEGSAAEQKTILLKFPQQMIDSEQLIL 896
                                                                   :::|.:.|
Mouse   374 -------------------------------------------------------EVMDVDDL-- 381

  Fly   897 QLGNLGKSHPCTSGFLSPPTSASRRPSVMFNEYVLLHTPPALNADPATAGTTTTVAPTVATVAGT 961
                       ..|.||
Mouse   382 -----------REGKLS------------------------------------------------ 387

  Fly   962 AAAAAAAAATGSGSGNNNHQNGGASGGAGTGGTTEKSNIFSTEKMTQAGDGSIWQVNLPQTIGTI 1026
                                                                             
Mouse   388 ----------------------------------------------------------------- 387

  Fly  1027 ANPYADCSELGIHDAMTCQELLAFSVAFSAALPTGQSTLESFYTSLARCDPHTAEALRAHHKPRS 1091
                                           |..|.|..||.|.
Mouse   388 -------------------------------LDEGGSDTESLYE--------------------- 400

  Fly  1092 VPTGQNQTTPGAEGATVMVASTAGDTGQTLQPSTVSAVVGGTIDSHAANHRRKEHHQQSHHHHNN 1156
                       .||.
Mouse   401 -----------IEGL-------------------------------------------------- 404

  Fly  1157 NNTTSHSRNYRSRQGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLIS 1221
            |......|::|    |.|...|..             |:||.:
Mouse   405 NKIIQFIRHWR----QWNRVFRWK-------------CHDLVK---------------------- 430

  Fly  1222 VYRCMSRVCSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGI 1286
                 |:|..|:                ::|.:.:||||:..|:||||..||.:.:.:|.|...:
Mouse   431 -----SKVFYWL----------------VILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTL 474

  Fly  1287 FAVEMLLKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRIL 1351
            |.:|||:|:...|..:|..:.||.||..:|....:||.....|   .....|:||||..||||:.
Mouse   475 FTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESG---AMSPLGISVLRCIRLLRLF 536

  Fly  1352 KLVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCP 1416
            |:.::..:|...:..:|.::.::|....||.|||.||::|||.||||:: .|.|
Mouse   537 KITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRY-DFED----------- 589

  Fly  1417 EIISRHPQCECDRKHFNNILWATVTVFQILTQEDWNVVLFNGM------EKTSHWAALYFVALMT 1475
                    .|..|.:|:|...|.::|||:||.||||.|::||:      ........:||:.|..
Mouse   590 --------TEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFV 646

  Fly  1476 FGNYVLFNLLVAILVEGF-------SSERNERREREQRELVKKLREETLAENYSDGMYDESRSEA 1533
            .|||:|.|:.:||.|:..       |:::.:..||::|::             |.|:.|:|..|.
Mouse   647 CGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKM-------------SKGLPDKSEEER 698

  Fly  1534 DSSTTNDSYYEVRNRWRSAEDVRKLQDSVELIIEAKSNMHRQRLLQPTHDYQINELPASSAAPSA 1598
            .:.|..   .|.:::........||:     |.|.:||::..:...|:.|:
Mouse   699 ATVTKK---LEQKSKGEGIPTTAKLK-----IDEFESNVNEVKDPYPSADF-------------- 741

  Fly  1599 SGTSGASAPGDRERDRDRDRDRERDRDRDRERDREAGGGGKEEGAQHAKPRGGLKKTYSIKERRS 1663
                    |||.|.|
Mouse   742 --------PGDDEED-------------------------------------------------- 748

  Fly  1664 EAPRLSKIRLARDPPIITTTAATPQDSPSTTLEPGMSFRQWGDMEPPSPPSPSLLRPPNIFTGGQ 1728
                                                        ||..|.||   ||
Mouse   749 --------------------------------------------EPEIPVSP---RP-------- 758

  Fly  1729 RSLDEGIPSIDLIPPSPVLSHKPLNILNASQLGVGMGGGVPSTAGSSSSMHSVIIDDISKSSSST 1793
                                 :||..|...:..|
Mouse   759 ---------------------RPLAELQLKEKAV------------------------------- 771

  Fly  1794 AAATPIYVPTISSTADAQSQSHSLNDVSVGSSPGGEIPATGMSRNANTGASTSGSSSSERLPLAP 1858
                                                                            |
Mouse   772 ----------------------------------------------------------------P 772

  Fly  1859 PPQQGSFKQRLRRGSSKKRRASALALATDDNPARRTLDNQARRTQDEEEEQQQLNNGGDNSCLLR 1923
            .|:..||
Mouse   773 IPEASSF---------------------------------------------------------- 779

  Fly  1924 NSNAVVSGSSSGTKETNRLSPQNSIRRLSNTLSIGSGPVGSRRASACIFNSQVYQNLNQPPKLRP 1988
                                                                             
Mouse   780 ----------------------------------------------------------------- 779

  Fly  1989 GSGQRRMSSIELAFSKTSHLNLHNLEANRKSLSYTNSKMDLDKWNKSYGNLNEPDNMLQQYMEAR 2053
                                                                             
Mouse   780 ----------------------------------------------------------------- 779

  Fly  2054 DKRKNSISHYNLKKRLEEKELQQLQQLHQQQLLQQRQDSFSSTTQQQQQQLQQHRLSKDQQQLAM 2118
                                                                             
Mouse   780 ----------------------------------------------------------------- 779

  Fly  2119 QPHSMVPGGGERYSKLKMLIEQLTPKHFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLL 2183
                                                 :||...|:.|.:|...||..||.|.:||
Mouse   780 -------------------------------------FIFSPTNKIRVLCHRIVNATWFTNFILL 807

  Fly  2184 FIALNCITLAMERPNIPPSSTERLFLATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIM 2248
            ||.|:...||.|.| |...|.....|...:||||.|||||:.:|:...|.|....::..:.:||:
Mouse   808 FILLSSAALAAEDP-IRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNIL 871

  Fly  2249 DGSLVTISIIDLLMSLISESSPRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGN 2313
            |..:|.:|:|    |:..|||  ...::::.|:||.|||||.||||.|||.|||.:..::|.|||
Mouse   872 DLLVVAVSLI----SMGLESS--AISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGN 930

  Fly  2314 IVLICCTFFIIFGILGVQLFKGTFYYCEGENIKGVRNADECR--------------RIPGNVWTN 2364
            |||:......:|..:|||||||.||.|   |.......:|||              .:....|.:
Mouse   931 IVLVTTLLQFMFACIGVQLFKGKFYSC---NDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIH 992

  Fly  2365 RKYNFDDLGKALMSLFVLSSRDGWVNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVL 2429
            ..::||::..|:||||.:|:.:||..::|..:|:...|..|:.|......::||.:|:|:.||::
Mouse   993 NDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMM 1057

  Fly  2430 NMFVGVVVENFHRCREEQEKEEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKY 2494
            |:|||.|:..|....|.:.|..::.:..::.:|...|.|.:.   .|...:|.:..|..||||.|
Mouse  1058 NIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLR---CYIPKNPYQYQVWYVVTSSY 1119

  Fly  2495 FDLAIAAVIGLNVVTMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQ 2559
            |:..:.|:|.||.:.:.|::|.....:.:...|.|..||.:|.||..:||:|...:.|..|.||.
Mouse  1120 FEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNV 1184

  Fly  2560 LDVGIVLLSIVGIVLEELET------------------NTHQIIPINPTIIRVMRVLRIARVLKL 2606
            .|..||:.||:.::|.|::|                  :..:...|:....|:.||:|:   :||
Mouse  1185 FDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRL---VKL 1246

  Fly  2607 LKMANGIRALLDTVM---QALPQVGNLGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAH 2668
            |..|.|:|.||.|.:   ||||.|   .||..:||||:|.:|:::||::...|...   :..:.:
Mouse  1247 LNRAEGVRTLLWTFIKSFQALPYV---ALLIVMLFFIYAVIGMQMFGKIAMVDGTQ---INRNNN 1305

  Fly  2669 FANFGMAFLTLFRVATGDNWNGIM----KDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQ 2729
            |..|..|.|.|||.|||:.|..|:    ...|.|...|.|....:.|.:: .|..:|:.|.::..
Mouse  1306 FQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEHTCGTN-FAYYYFISFYMLCA 1369

  Fly  2730 FVLVNVVVAVLM---------------KHLEESHKQMEDELDMEVE 2760
            |:::|:.|||:|               .||:| .|.:..|.|.|.:
Mouse  1370 FLIINLFVAVIMDNFDYLTRDWSILGPHHLDE-FKAIWAEYDPEAK 1414

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1T	NP_001259267.1	Ion_trans	300..636	CDD:459842	98/353 (28%)
		Ion_trans	1243..1495	CDD:459842	86/264 (33%)
		Ion_trans	2175..2448	CDD:459842	106/286 (37%)
		Ion_trans	2492..2728	CDD:459842	81/260 (31%)
Cacna1s	NP_001074492.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..23		3/7 (43%)
		I. /evidence=ECO:0000305	38..337		96/357 (27%)
		Ion_trans	50..345	CDD:459842	99/355 (28%)
		Selectivity filter of repeat I. /evidence=ECO:0000250|UniProtKB:P07293	290..293		1/2 (50%)
		Binding to the beta subunit. /evidence=ECO:0000269|PubMed:28351836	357..374		6/191 (3%)
		II. /evidence=ECO:0000305	418..664		94/324 (29%)
		Ion_trans	431..672	CDD:459842	89/279 (32%)
		Selectivity filter of repeat II. /evidence=ECO:0000250|UniProtKB:P07293	612..615		1/2 (50%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	675..712		10/52 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	731..757		12/144 (8%)
		III. /evidence=ECO:0000305	768..1068		116/694 (17%)
		Ion_trans	798..1075	CDD:459842	106/286 (37%)
		Dihydropyridine binding. /evidence=ECO:0000250|UniProtKB:P07293	988..1077		29/88 (33%)
		Selectivity filter of repeat III. /evidence=ECO:0000250|UniProtKB:P07293	1012..1015		0/2 (0%)
		IV. /evidence=ECO:0000305	1105..1384		92/288 (32%)
		Ion_trans	1117..1392	CDD:459842	87/284 (31%)
		Selectivity filter of repeat IV. /evidence=ECO:0000250|UniProtKB:P07293	1321..1324		2/2 (100%)
		Dihydropyridine binding. /evidence=ECO:0000250|UniProtKB:P07293	1337..1403		17/67 (25%)
		Phenylalkylamine binding. /evidence=ECO:0000250	1349..1392		10/43 (23%)
		Phenylalkylamine binding. /evidence=ECO:0000250|UniProtKB:P07293	1349..1391		10/42 (24%)
		GPHH	1401..1454	CDD:465306	5/15 (33%)
		Ca_chan_IQ	1464..1536	CDD:462591
		Interaction with calmodulin. /evidence=ECO:0000250|UniProtKB:Q13698	1522..1542
		CAC1F_C	1557..1840	CDD:465298
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1702..1721
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1727..1762