Protein Alignment Ca-alpha1T and Cacna1b

DIOPT Version :10

• Sequence 1: NP_001259267.1
• Gene: Ca-alpha1T / 31550
• FlyBaseID: FBgn0264386
• Length: 3218
• Species: Drosophila melanogaster
• Sequence 2: XP_006497689.1
• Gene: Cacna1b / 12287
• MGIID: 88296
• Length: 2351
• Species: Mus musculus
• Alignment Length: 3271
• Identity: 688/3271 - (21%)
• Similarity: 1071/3271 - (32%)
• Gaps: 1280/3271 - (39%)

  Fly   228 GGRTGGAVSSSTGSSCNGSCSSDDGDSSHTSSSSSSSSSGEPNLPYPGFPEISIRALTQYTRPRN 292
            |||.||     ||.          |:.:....:..:...|:..|| ||...:..:::.|..|
Mouse     9 GGRYGG-----TGG----------GERARGGGAGGAGGPGQGGLP-PGQRVLYKQSIAQRAR--- 54

  Fly   293 WCLMLITNP--------------------------------W--FERVSILVILLNCITLGMYQ- 322
              .|.:.||                                |  ||.:.:..|:.|||.|.:.|
Mouse    55 --TMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQH 117

  Fly   323 -PCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMG-ICGKNTYLADSWNRLDFFIVLAGLL 385
             |..|...::.|   |...:......|..|..||::|:| :..|.:||.:.||.:||.:||.|:|
Mouse   118 LPDGDKTPMSER---LDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGIL 179

  Fly   386 EYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCFFVFFIFGIIGV 450
              .....:.:|..:|.:||||||:.::.|||:::::..::..:..|..:.||.||...:|.|||:
Mouse   180 --ATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGL 242

  Fly   451 QLWEGILRQRCSLMRTEGMVYPLTVPEVYRVRVRVNSLSQYYEFSKDQDYICSTPNDSGMHLCGN 515
            :.:.|...:.|                              :..|.|.:.:...|       ||.
Mouse   243 EFYMGKFHKAC------------------------------FPNSTDTEPVGDFP-------CGK 270

  Fly   516 FPPYRIGSLVCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGMAWVAIFLV 580
            .||.|    .|:.:                     |.|::....|..|..:||||..|.:.:|..
Mouse   271 DPPAR----QCDGD---------------------TECREYWPGPNFGITNFDNILFAILTVFQC 310

  Fly   581 ISLEGWTDIMYYVQD-AHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETKKREMERMRQERA 644
            |::||||||:|...| |.:.|:|:||:.||:||||||:||.|.|::.:|:    :|.||:...||
Mouse   311 ITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFA----KERERVENRRA 371

  Fly   645 RYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKYQRQQRKEGLLPNADNLT 709
                                                        ..|.:|||:.|..|.......
Mouse   372 --------------------------------------------FLKLRRQQQIERELNGYLEWI 392

  Fly   710 FSPSRIKCHHPKCPKYSNRKPSSIQDQMITVMVPLNSASNNINNNNNNNSNSSNHNGSGNHTAAG 774
            |....:
Mouse   393 FKAEEV----------------------------------------------------------- 398

  Fly   775 GGNNNNNNNNNNAASCTTVALVNGINGSAASVTMSSAHHQQHQLLQHQQQQHQNQQQQQQQQQHS 839
                                                                             
Mouse   399 ----------------------------------------------------------------- 398

  Fly   840 SSDNTEQSLAPDGLPRSSSLKKSTAHHQLKPEGSAAEQKTILLKFPQQMIDSEQLILQLGNLGKS 904
                                       .|..|...||:|
Mouse   399 ---------------------------MLAEEDKNAEEK-------------------------- 410

  Fly   905 HPCTSGFLSPPTSASRRPSVMFNEYVLLHTPPALNADPATAGTTTTVAPTVATVAGTAAAAAAAA 969
                    ||..:..:|.:...:...|:|
Mouse   411 --------SPLDAVLKRAATKKSRNDLIH------------------------------------ 431

  Fly   970 ATGSGSGNNNHQNGGASGGAGTGGTTEKSNIFSTEKMTQAGDGSIWQVNLPQTIGTIANPYADCS 1034
                                                   |.:|....|:|    ..:.:|:|
Mouse   432 ---------------------------------------AEEGEDRFVDL----CAVGSPFA--- 450

  Fly  1035 ELGIHDAMTCQELLAFSVAFSAALPTGQSTLESFYTSLARCDPHTAEALRAHHKPRSVPTGQNQT 1099
                                .|:|.:|::...|::
Mouse   451 --------------------RASLKSGKTESSSYF------------------------------ 465

  Fly  1100 TPGAEGATVMVASTAGDTGQTLQPSTVSAVVGGTIDSHAANHRRKEHHQQSHHHHNNNNTTSHSR 1164
                                                      ||||
Mouse   466 ------------------------------------------RRKE------------------- 469

  Fly  1165 NYRSRQGQGNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRV 1229
                                                                             
Mouse   470 ----------------------------------------------------------------- 469

  Fly  1230 CSWIRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEMLLK 1294
             ...|.:|||:|:.:.|...:|..:.:|||.:.:.::|||..||..:..:..||.|:|..||.||
Mouse   470 -KMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLK 533

  Fly  1295 VVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILKLVRFMPN 1359
            :...||..|..:.||.||..:::.|..|:....:..||   ..|:||||..|||||.|:.::..:
Mouse   534 MYGLGPRSYFRSSFNCFDFGVIVGSIFEVVWAAIKPGT---SFGISVLRALRLLRIFKVTKYWNS 595

  Fly  1360 LRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMYLFGGKFCKFLDESGLERECTCPEIISRHPQ 1424
            ||..:..:|.:|.::.....||.|||.:|::|||.||||:| .|.||:
Mouse   596 LRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQF-NFQDET----------------- 642

  Fly  1425 CECDRKHFNNILWATVTVFQILTQEDWNVVLFNGME-----KTSHWAALYFVALMTFGNYVLFNL 1484
               ...:|:....|.:|||||||.||||.|:::|:|     ....:::.||:.|..||||.|.|:
Mouse   643 ---PTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNV 704

  Fly  1485 LVAILVEGFSSERNERREREQRE-------LVKKLRE-----ETLAENYSDGMYDE------SRS 1531
            .:||.|:..::.:...::.|:.|       .::|.:|     ...|.|.|...:.:      |||
Mouse   705 FLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAFVKQTRGTVSRS 769

  Fly  1532 EADSSTTN---DSYYEVRNRWRSAEDVRKLQD---SVELII-------------------EAKSN 1571
            .:.||..:   .:..:.|:.|.......:||:   |.|.:.                   :.|::
Mouse   770 SSVSSVNSPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRYASTRHVRPDMKTH 834

  Fly  1572 MHRQRLLQPTHDYQINELPASSAAPSASGTSGASAPGDRERDRDRDRDR-------ERDRDRDRE 1629
            |.|..:::|..|.....:.:.|.......|..|..|....|.||||:..       |:||....|
Mouse   835 MDRPLVVEPGRDGLRGPVGSKSKPEGTEATESADLPRRHHRHRDRDKTSATAPAGGEQDRTESTE 899

  Fly  1630 RDREAGGGGKEEGAQHAKPRGGLKKTYSIKERRSEAPRLSKIRLARDPPIITTTAATPQDSPSTT 1694
            .      |.:||   .|:||    :::| ||                         ||.......
Mouse   900 T------GAREE---RARPR----RSHS-KE-------------------------TPGADTQVR 925

  Fly  1695 LEPGMSFRQWGDMEPPSPPSPSLLRPPNIFTGGQRSLDEGIPSIDLIPPSPVLSHKPLNILNASQ 1759
            .|......:.|..|..:...|...|                            :|:
Mouse   926 CERSRRHHRRGSPEEATEREPRRHR----------------------------AHR--------- 953

  Fly  1760 LGVGMGGGVPSTAGSSSSMHSVIIDDISKSSSSTAAATPIYVPTISSTADAQSQSHSLNDVSVGS 1824
                               |:        ..||.....|:.||.....|..:.
Mouse   954 -------------------HA--------QDSSKEGTAPVLVPKGERRARHRG------------ 979

  Fly  1825 SPGGEIPATGMSRNANTGASTSGSSSSERLP--LAPPPQQGSFKQRLRRGSSKKRRASALALATD 1887
                  |.||.....|....|....:..::|  |.||.::.:.|:
Mouse   980 ------PRTGPREAENNEEPTRRHRARHKVPPTLQPPEREAAEKE-------------------- 1018

  Fly  1888 DNPARRTLDNQARRTQDEEEEQQQLNNGGDNSCLLRNSNAVVSGSSSGTKETNRLSPQNSIRRLS 1952
                                                 ||||     .|.|||....|:.....|.
Mouse  1019 -------------------------------------SNAV-----EGDKETRNHQPKEPHCDLE 1041

  Fly  1953 NTLSIGSGPVGSRRASACIFNSQVYQNLNQPPKLRPGSGQR---RMSSIELAFSKTSHLNLHNLE 2014
            .....|.||: ....|.|:      |.:::.|:  ....||   ||.|.....|.|.|:.: .|.
Mouse  1042 AIAVTGVGPL-HMLPSTCL------QKVDEQPE--DADNQRNVTRMGSQPSDPSTTVHVPV-TLT 1096

  Fly  2015 ANRKSLSYTNSKMDLDKWNKSYGNLNEPDNMLQQYMEARDKRKNSISHYNLKKRLEEKELQQLQQ 2079
            ..........|           ||:|     |:...|.             ||..|..::
Mouse  1097 GPPGETPVVPS-----------GNMN-----LEGQAEG-------------KKEAEADDV----- 1127

  Fly  2080 LHQQQLLQQRQDSFSSTTQQQQQQLQQHRLSKDQQQLAMQPHSMVPGGGERYSKLKMLIEQLTPK 2144
                                                |...|..:||     ||
Mouse  1128 ------------------------------------LRRGPRPIVP-----YS------------ 1139

  Fly  2145 HFTTEREDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFL 2209
                     |::.....|..|:.|.:.|..::|:.|:|:.|||:.|.||.|.| :...|.....|
Mouse  1140 ---------SMFCLSPTNLLRRFCHYIVTMRYFEMVILVVIALSSIALAAEDP-VRTDSFRNNAL 1194

  Fly  2210 ATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMD-----GSLVTISIIDLLMSLISESS 2269
            ...:|:||.|||.||.||::..|:.....|||...|||:|     |:||..:    ..|.:..|.
Mouse  1195 KYMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFA----FSSFMGGSK 1255

  Fly  2270 PRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILGVQLFK 2334
            .:....::..|:||.||||:.|.|.|.||.|...:::||:.:.||:::...|..||.::.|||||
Mouse  1256 GKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFK 1320

  Fly  2335 GTFYYCEGENIKGVRNADECR-----------RIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGW 2388
            |.|:||..|:.:..|   :||           ......|....:::|::..||::||.:|:.:||
Mouse  1321 GKFFYCTDESKELER---DCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGW 1382

  Fly  2389 VNIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEKEEKI 2453
            ..::...:||...:|.|...:.....::::.:.::..||.:|:||.:::..|      ||:.:|:
Mouse  1383 PMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVFPFFFVNIFVALIIITF------QEQGDKV 1441

  Fly  2454 RRAAKRALQMEKKRR-----RMHEPPYYTNYSP--TRMFVHNV---VTSKYFDLAIAAVIGLNVV 2508
            ....    .:||..|     .:...| .|.|.|  .:.|.:..   |.|..|:..|.|:|.||.|
Mouse  1442 MSEC----SLEKNERACIDFAISAKP-LTRYMPQNKQSFQYKTWTFVVSPPFEYFIMAMIALNTV 1501

  Fly  2509 TMAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSIVGIV 2573
            .:.|::|..|...:..||..|..||::|.:|..:|::|.|...|.:|.||..|...||.||..|:
Mouse  1502 VLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFDFVTVLGSITDIL 1566

  Fly  2574 LEEL-ETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGNLGLLFFLL 2637
            :.|: |||..    ||   :..:|:.|.||::|||:....||.||.|.:|:...:..:.||..:|
Mouse  1567 VTEIAETNNF----IN---LSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAML 1624

  Fly  2638 FFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWNGIMKDTLRDN-CD 2701
            |||:|.:|:::||.:...|:   ..:..|.:|..|..|.:.|||.|||:.|:.||...|.:. ||
Mouse  1625 FFIYAIIGMQVFGNIALDDD---TSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLGNRACD 1686

  Fly  2702 ---DAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLM---------------KHLEE-- 2746
               :|::|      .|..|..:||.|:.:..|:::|:.|||:|               .||:|
Mouse  1687 PHANASEC------GSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFI 1745

  Fly  2747 --------------SHKQMEDEL--------------------------------DMEVELEREL 2765
                          |:..|.:.|                                ||.|.....|
Mouse  1746 RVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTL 1810

  Fly  2766 VREQEFAQEQKLC-----QQLAEAQSK-------AAAPPR------PLAKVKSLPKNFIYST--- 2809
            :.....|.|.||.     |...:|:.:       |..|.:      |..|...:....:|:.
Mouse  1811 MALIRTALEIKLAPAGTKQHQCDAELRKEISSVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMI 1875

  Fly  2810 --------PSLDKKFPAVSGVN------------------NPSLTSSAATNLNQ-VATAGSGGAA 2847
                    .:.|:...|..|::                  .|::...|...|.| .||:.|.|.|
Mouse  1876 FDFYKQNKTTRDQTHQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSATSLSNGGA 1940

  Fly  2848 PGAVPAG-----SGAGPRRQTVQYFQQPPQSMLGGLSLAEM----GGTLTPQALGARL------- 2896
            .....:|     |....|.|...|..:.|  :..|.| ||:    .|||   |:..::
Mouse  1941 IQTQESGIKESLSWGTQRTQDALYEARAP--LERGHS-AEIPVGQSGTL---AVDVQMQNMTLRG 1999

  Fly  2897 --GEPFGG---GGGNKSGPRRQSYWQINPIRKRGVLSKERSLDEQAIRRRNLEAKRTSCDSLPWG 2956
              |||..|   .|...|.||..:..|..|    .....:||:...|.|....:...|..|..|..
Mouse  2000 PDGEPQPGLESQGRAASMPRLAAETQPAP----NASPMKRSISTLAPRPHGTQLCSTVLDRPPPS 2060

  Fly  2957 GDALD----C--RRGTIFESLESDGGGVG----GGGSGGGGGGLGGGV-SYDLRSVRSADVGHSE 3010
            ..:..    |  ||.....|||. |..:.    |..|...|.||..|. |...|..|..:.|.|:
Mouse  2061 QASHHHHHRCHRRRDKKQRSLEK-GPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRKQERGRSQ 2124

  Fly  3011 MDGDVSLSVVS----------------ALVPSVTT-PLPPPLSL---------------PIVTPT 3043
            .....|.|...                .|:||::: |..|..:|               |:::.:
Mouse  2125 ERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTSPTAALEPAPHPQGSGSVNGSPLMSTS 2189

  Fly  3044 STPTP-----LQLPM-PM-PMP-----MAHPPPRRPFGWSQS---------VDQGCLRSNLLL-S 3086
            ...||     .|||. |: |.|     .|:..|.. |...||         :.:|....|.|| .
Mouse  2190 GASTPGRGGRRQLPQTPLTPRPSITYKTANSSPVH-FAEGQSGLPAFSPGRLSRGLSEHNALLQK 2253

  Fly  3087 VPRSMP--PRSRSGSTKQLFKQQALDEDAD---MDENSLLLPTAAGGGSGPGSVAVIASSSLDLP 3146
            .|.|.|  |.||.||...|  .|.||.:|.   :.|::|....|....||..|.....||   |.
Mouse  2254 EPLSQPLAPGSRIGSDPYL--GQRLDSEASAHTLPEDTLTFEEAVATNSGRSSRTSYVSS---LT 2313

  Fly  3147 DQSGSSKQILPD---IALGVS 3164
            .||...::: |:   ..||:|
Mouse  2314 SQSHPLRRV-PNGYHCTLGLS 2333

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1T	NP_001259267.1	Ion_trans	300..636	CDD:459842	100/373 (27%)
		Ion_trans	1243..1495	CDD:459842	89/256 (35%)
		Ion_trans	2175..2448	CDD:459842	88/288 (31%)
		Ion_trans	2492..2728	CDD:459842	84/240 (35%)
Cacna1b	XP_006497689.1	Ion_trans	94..367	CDD:459842	101/343 (29%)
		Ion_trans	482..721	CDD:459842	89/262 (34%)
		Granin	<936..>1041	CDD:279595	31/248 (13%)
		Ion_trans	1161..1395	CDD:459842	80/241 (33%)
		Ion_trans	1485..1734	CDD:459842	90/264 (34%)
		GPHH	1743..1796	CDD:465306	4/52 (8%)
		Ca_chan_IQ	1807..1884	CDD:462591	12/76 (16%)
		PHA03307	1959..>2326	CDD:223039	97/384 (25%)