DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment spoon and Akap1

DIOPT Version :9

Sequence 1:	NP_726992.2	Gene:	spoon / 31459	FlyBaseID:	FBgn0263987	Length:	607	Species:	Drosophila melanogaster
Sequence 2:	NP_001036006.1	Gene:	Akap1 / 11640	MGIID:	104729	Length:	857	Species:	Mus musculus

Alignment Length:	890	Identity:	188/890 - (21%)
Similarity:	316/890 - (35%)	Gaps:	356/890 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 RPLLYLSLPGVAFILGVFWFRRRYKN--------------------------------------- 52
            |.|..|:|||:..:||.:||..|.|:
Mouse     6 RSLFPLALPGMLALLGWWWFFSRKKDRLSSSDKQVETLKVGPAIKDRRLSEEACPGVLSVAPTVT 70

  Fly    53 ---------CLDKPDDE--------------DSS----AINDSSIEP------------------ 72
                     |:|||..|              :||    ::.|:..:|
Mouse    71 QPPGREEQRCVDKPSTEPLALPRTRQVRRRSESSGNLPSVADTRSQPGPCRDEIAKVELSLMGDK 135

  Fly    73 --------------------TVQARKANGVLQNGKLPQQ--------SASKSMNINGTLVNGSGS 109
                                .|:..|....|  ||.|.:        |..|:....||  .|:|.
Mouse   136 AKSIPLGCPLLPKDASFPYEAVERCKQESAL--GKTPGRGWPSPYAASGEKARETGGT--EGTGD 196

  Fly   110 -------------------------------GSGSSSDEKDSPTTM---------LYGK------ 128
                                           |.|...:....|..:         :.||
Mouse   197 AVLGENVSEEGLLSQECVSEVEKSEFPILAPGGGEGEEVSHGPPQVAELLKKEEYIVGKLPSSFV 261

  Fly   129 ----SAPIK--------IQSNGRTSNGKHQQQIDSEIL---KSKIQDAEHKTLCSI----DEDFE 174
                |.|:|        ::.:..||:.....::|.:..   ..:|:.|..:.:..:    .|:|.
Mouse   262 EPVHSEPVKDEDALEPQVKGSSNTSDRDLAGELDKDETVPENDQIKQAAFQLISQVILEATEEFR 326

  Fly   175 --------------NLSSPRD-----LPDSVNTRVSFYNRKATQKTVEPVVIKATRT-------- 212
                          :.:.|:.     :|.|..|.:.       |.|.:|.   :|||
Mouse   327 ATTVGKTVAQVHPTSATQPKGKEESCVPASQETSLG-------QDTSDPA---STRTGATASPSA 381

  Fly   213 --------------------------------------PKISPENSFL--------DTNYTNKEC 231
                                                  |.::|:...|        |.|:.  .|
Mouse   382 EALPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVTPQRQSLEGASNPRGDDNFV--AC 444

  Fly   232 EQNNN-----------C-EPKEEPSKKEADQEELEQ--DQEQT-------------VLKEEVVD- 268
            ..||:           | :|......:::..|.:..  |:..|             |||||:.|
Mouse   445 MANNSQSVLSVSSLGQCSDPVSTSGLEDSCTETISSSGDKAMTPPLPVSTQPFSNGVLKEELSDL 509

  Fly   269 -----------------SNGNQKRNVDAA-------SPSLSICSVQSGDSGKGSSLPRSEATRVK 309
                             |:||...:||:.       ||.    |||:|.:.|...|
Mouse   510 GTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQ----SVQAGSNPKKVDL--------- 561

  Fly   310 TTYEFLFPISLIGHLYGRKRAFINQIKAKTLASVSVGKNPYSGKVRICTIEGTESEIDAALAMIR 374
            ..:|...|..|:|.|.|::..:::.:|..:.|.:.:...||:..::||.|||::..:|.||.:|
Mouse   562 IIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLI- 625

  Fly   375 QRLPAKRYPNFTMQRIH------FALPQTIVPLSTESLYNLQLKLIEGINNDVVVSAVLSGSHIF 433
                .|::....:..|:      .|||.  :|:::      .|.|.:||..:|:|...::..|:|
Mouse   626 ----GKKFKELNLTNIYAPPLPSLALPS--LPMTS------WLMLPDGITVEVIVVNQVNAGHLF 678

  Fly   434 IQHPLHPSHPSLPLLQKQLYDSYSTMEAPLLPS-LELSAVCVIPIND-VWYRVQIVDTDPEDEER 496
            :|...||:..:|..|.:|:|..||....|.||: :|::.:|..|..| .|:|.|:|.:..|..|
Mouse   679 VQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNE- 742

  Fly   497 CVIKFLDFGGYMNVGFNTLRQIRTDFMNVPFQSTECILSNIEPIGGT--WSIEAAEILNKLTKGI 559
            ..|:::|:|||..|..:.|||||:||:.:|||..|.:|.::.|:...  :|.||...::::|...
Mouse   743 VEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNT 807

  Fly   560 VLQAQVAGYNSHNLPEIFLFASLGPNNVIFINKELVGRKLAKWVE 604
            .|.|||..|::..||.|.|::.:| :.|:.||:.||.|.||:||:
Mouse   808 ALLAQVTSYSATGLPLIQLWSVVG-DEVVLINRSLVERGLAQWVD 851

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
spoon	NP_726992.2	KH-I	311..373	CDD:238053	18/61 (30%)
		TUDOR	413..536	CDD:278965	47/124 (38%)
		TUDOR	465..522	CDD:295375	22/58 (38%)
Akap1	NP_001036006.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	65..121		9/55 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	165..198		10/36 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	260..303		6/42 (14%)
		PKA-RII subunit binding domain	306..319		2/12 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	336..437		13/110 (12%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	466..497		3/30 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	512..554		10/45 (22%)
		KH-I	565..625	CDD:238053	18/59 (31%)
		TUDOR	658..780	CDD:278965	46/122 (38%)
		TUDOR	710..768	CDD:197660	22/58 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167849583
Domainoid	1	1.000	88	1.000	Domainoid score	I7953
eggNOG	1	0.900	-	-		E1_KOG2279
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	193	1.000	Inparanoid score	I3834
Isobase	1	0.950	-	0	Normalized mean entropy	S7503
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0006304
OrthoInspector	1	1.000	-	-		oto94610
orthoMCL	1	0.900	-	-		OOG6_107934
Panther	1	1.100	-	-	O	PTHR22948
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2941
SonicParanoid	1	1.000	-	-		X4588
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.730