DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Usp16-45 and USP4

DIOPT Version :9

Sequence 1:	NP_001284890.1	Gene:	Usp16-45 / 31458	FlyBaseID:	FBgn0029763	Length:	1126	Species:	Drosophila melanogaster
Sequence 2:	NP_003354.2	Gene:	USP4 / 7375	HGNCID:	12627	Length:	963	Species:	Homo sapiens

Alignment Length:	1056	Identity:	223/1056 - (21%)
Similarity:	351/1056 - (33%)	Gaps:	353/1056 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   153 YECDMKICSNL-RKNLLEC----------VEL-VKKLAQKPP-------TSTVTPSTPTISYIEE 198
            |..::|:|.|. ..|:|.|          :|. ::||...|.       ...::.:...:|.::.
Human   136 YLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDN 200

  Fly   199 KLKAALEHLTPIVPMTGGSFDDSSSRGSLAAAGGGGGVGSSRNRQVAIPMPPPEPSSGLSTS--- 260
            .::.|..:...::.:...:.|.:..|.:|.:.   .....|||...: |.....|.|.:|.|
Human   201 TVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSK---SSTAPSRNFTTS-PKSSASPYSSVSASLIA 261

  Fly   261 --DSLTSVPGMAKRIDQYSATTNGNTGNKRLLTLETPRIENERLPRVRGLTNLGNTCFFNAVMQC 323
              || ||..||     ..|..:.|.:|.......:.|...:.: |.:.||.|||||||.|:.:||
Human   262 NGDS-TSTCGM-----HSSGVSRGGSGFSASYNCQEPPSSHIQ-PGLCGLGNLGNTCFMNSALQC 319

  Fly   324 LAQTPFLLSVLKELSEPGEEFILPGGTFTIKDKGDIELPMIKGTLSSWGGLTAALANALEELQAG 388
            |:.|                  .|...:.:||:.:.|:.. ...|...|.:..|.|..::::.:|
Human   320 LSNT------------------APLTDYFLKDEYEAEINR-DNPLGMKGEIAEAYAELIKQMWSG 365

  Fly   389 GSVFTPRKLFDRLCVK-CPQFTGGDQHDAHELLRQLLESVRNEDLKRYQRVILQNLGYKDQDVNS 452
            .......::|.....: .|||:|..|.|:.|||..||:.: :|||.|.::.....|    :|.|.
Human   366 RDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGL-HEDLNRVKKKPYLEL----KDANG 425

  Fly   453 VSEEMRQKCKIYGNQAGDRILRPEQV----FRGFLVSTLTCQDCHNVSSRHEYFLDMSLPVAVEK 513
            ..:.:..| :.:.|..    ||.:.|    |.|...|||.|.:|..||...:.|..::||:.::|
Human   426 RPDAVVAK-EAWENHR----LRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKK 485

  Fly   514 P--------------QPPQRRKPSPELSLTS---------SSSSVTPSTGQPTINTKF-----TD 550
            .              :|.|.|...|.:...|         |..:..........|.:|     .|
Human   486 DRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMD 550

  Fly   551 GSVNFTASSPSFFLHAHEAASLGPSKSQVKKEKERQRKAKRAAKKRQKSSLNLNGNDSGNGNELA 615
            ..:|........|::...:.|:..|:........|:||: |.:.....|:|        .|..|.
Human   551 EGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKS-RPSSTSSASAL--------YGQPLL 606

  Fly   616 ESVDQDDASLASLGAGDGQANDGLEQTNGQTEDSTTSSVTTSEHSDADVEDNLVEDTAAPSTNNV 680
            .||.:...:|.||    .||                            |.|.:......|..:..
Human   607 LSVPKHKLTLESL----YQA----------------------------VCDRISRYVKQPLPDEF 639

  Fly   681 PSSTASLTAPSKTYMDSNGNAQPPGEKRDDTPEHMD--KDSLEEDE--------NDSGIAT---- 731
            .||.....|       .||:......:.::..||.:  |:.|.|.|        ||....|
Human   640 GSSPLEPGA-------CNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKI 697

  Fly   732 --SPAPTA--TNSSTSTSATGNNNSVAGSGL-----SGSSGALEDPLAPASLVTAGLSEKGASVI 787
              .|.|..  |.|..::..|.:.||:|..|.     |.|:.|:
Human   698 KGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAM---------------------- 740

  Fly   788 RQVSVGAEQGASNGTEDADGEAKAI---EQPEKTPSQAQAMAQAQARTKRVRTQSYSDWSTTIAP 849
                            |.|.|.:.:   ||..:...:..:|.|.|.:.|           ||:|
Human   741 ----------------DWDSETRRLYYDEQESEAYEKHVSMLQPQKKKK-----------TTVA- 777

  Fly   850 RYQCEDGECSVQSCLNNFTAVELMTGQNKVGCDSCTQRINGSDPKAKSVNTNATKQLLVSSPPAV 914
                      ::.|:..||.:|.:...:...|.:|.:            :..|||:..:.|.|.:
Human   778 ----------LRDCIELFTTMETLGEHDPWYCPNCKK------------HQQATKKFDLWSLPKI 820

  Fly   915 LILHLKRFQLGPRCIFRKLTRPVSYP-NLLDIAAFCGSKVKNLPNIDRKQKKLLYALYGVVEHSG 978
            |::|||||... |....||...|.:| ..|:::.|    |.||     ..:..:|.|..|..|.|
Human   821 LVVHLKRFSYN-RYWRDKLDTVVEFPIRGLNMSEF----VCNL-----SARPYVYDLIAVSNHYG 875

  Fly   979 GMYGGHYTAYVKVRPKVAPGDKRWKFLPHGSKAELDQDDDQLKKLEELLAKEKAREQHLKVLDDS 1043
            .|..||||||.|                              .||.
Human   876 AMGVGHYTAYAK------------------------------NKLN------------------- 891

  Fly  1044 DDFSNSSSNSSTSDESQTPATPLEEQQTQQAQQPQQPQQLEEAANVRAPPGKWYYVSDSRVQEVS 1108
                                                              |||||..||.|...|
Human   892 --------------------------------------------------GKWYYFDDSNVSLAS 906

  Fly  1109 EDTALKAQAYLLFYER 1124
            ||..:...||:|||:|
Human   907 EDQIVTKAAYVLFYQR 922

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp16-45	NP_001284890.1	zf-UBP	108..159	CDD:280334	1/5 (20%)
		Peptidase_C19	307..>512	CDD:271592	59/209 (28%)
		Peptidase_C19K	<849..1123	CDD:239132	57/274 (21%)
USP4	NP_003354.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..22
		Necessary for interaction with SART3. /evidence=ECO:0000269\|PubMed:20595234	27..216		13/79 (16%)
		Peptidase_C19	35..923	CDD:321984	223/1056 (21%)
		Nuclear export signal. /evidence=ECO:0000250\|UniProtKB:P35123	133..141		1/4 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	219..253		8/37 (22%)
		Required for USP4 activation by providing conformational flexibility between the DUSP and catalytic domains. /evidence=ECO:0000269\|PubMed:25404403	229..295		19/75 (25%)
		Regulates ubiquitin dissociation. /evidence=ECO:0000269\|PubMed:25404403	384..386		1/1 (100%)
		Necessary for interaction with RBL2. /evidence=ECO:0000250\|UniProtKB:P35123	405..407		0/2 (0%)
		Necessary for interaction with RB1 and RBL2. /evidence=ECO:0000250\|UniProtKB:P35123, ECO:0000269\|PubMed:11571652	459..463		2/3 (67%)
		Interacts with DUSP and ubiquitin-like 1 domains and is required for USP4 activation. /evidence=ECO:0000269\|PubMed:25404403	485..775		67/386 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	637..699		14/68 (21%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:P35123	767..772		2/4 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	928..963
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5560
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.900