DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment norpA and plch2b

DIOPT Version :9

Sequence 1:	NP_001014720.1	Gene:	norpA / 31376	FlyBaseID:	FBgn0262738	Length:	1095	Species:	Drosophila melanogaster
Sequence 2:	XP_017213827.1	Gene:	plch2b / 560919	ZFINID:	ZDB-GENE-101112-1	Length:	1260	Species:	Danio rerio

Alignment Length:	1024	Identity:	266/1024 - (25%)
Similarity:	442/1024 - (43%)	Gaps:	218/1024 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 EFDWI---IPVPPELTTGCVFDRWFEN--------EKETKENDFERDALFKVDEYGFFLYWKSEG 59
            ||.||   |...|:...|.:.:|...|        :...|.....|  ||.:||:...:.|:...
Zfish    59 EFFWIGGSIVASPKWRMGQIVERCMCNMQAGSQMIKLRGKSKALVR--LFYLDEHKSCIRWRPSR 121

  Fly    60 RD-------GDVIELCQ--VSDI--RAGGTPKDPKILDKVTKKNGTNIPELDKRSLTICSNTDYI 113
            :.       ..:.|:|:  .:::  |......||.....:  .:|.::..||
Zfish   122 KHERAKITIDSIHEVCEGMRTEVFRRFANNNFDPNCCFSI--YHGDHVESLD------------- 171

  Fly   114 NITYHHVICPDAATAKIWLDGIRKITHNVK-ANNICPMMCLRKHWMRLSYC-VEKSGKIPVKTLA 176
                  ::..:...|:.|:.|::.:...:. .:::......|..|:|.::. .:|:|.
Zfish   172 ------LVSTNGEEARTWITGLKYLLAGISDEDSLAKRQRTRDQWLRQTFSEADKNGD------- 223

  Fly   177 KTFASGKTEKLVYTCIKDAGLPDDKNATMTKE--------QFTFDKFYALYHKVCPRNDIEELFT 233
            ...:.|:..:|::..  :..||..|...|.:|        ..||::|...|..:..|.|:..|..
Zfish   224 GNLSIGEVLQLLHKL--NVNLPKQKVREMFQEADTDENQGSLTFEEFCTFYKLISTRRDLYLLMI 286

  Fly   234 SITKGKQDFISLEQFIQFMNDKQRDPRMNEILYPLYEEKRCTEIINDYELDEEKKKNVQMSLDGF 298
            |.:..| :.:.|....:|:..:|:...:.        ::.|..|||.:|...:.:.:|.:.:|||
Zfish   287 SYSNHK-EHLDLNDLQRFLESEQKMVNVT--------KEYCKMIINQFEPCSQNQSHVVLGIDGF 342

  Fly   299 KRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCV 363
            ..|:.|........:......:|.|||.:|||.|||||||:|.|:..:|.|:||...|..|||||
Zfish   343 TNYMRSPAGDIFNPEHYQVNQDMTQPLTNYYIASSHNTYLTGDQLLSQSRVDMYAYVLQMGCRCV 407

  Fly   364 ELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAK 428
            |:|||:  |.|.||||.||:...::|||||.::.|...||..:::|||||.||||...||.|:|:
Zfish   408 EVDCWD--GPDGEPIVHHGYTLTSKILFKDVVETINKYAFAKTQFPVILSIENHCTVPQQKKMAQ 470

  Fly   429 YCDDFFGDLLLKEPLPDHPLDPGLPLPPPCKLKRKILIKNKRMKPEVEKVELELWLKGELKTDDD 493
            |..:...|   |..|.:...:....||.|..||.|||:|.|::...::....|    |::..:|.
Zfish   471 YLMEVLQD---KVDLSNINTNESRRLPSPEDLKGKILVKGKKLPANIDADADE----GDVSDEDS 528

  Fly   494 PEEDASA-----GKPPEAAAAPAPAPEAAAAAEGAAEGGGGAEAEAAAANYSG------------ 541
            .|||...     |...|.      |||:|:|.|         ..:.:...:.|
Zfish   529 GEEDEEEELQLNGDTTEG------APESASADE---------SKKRSRRKFMGSFRRKKKKQVKV 578

  Fly   542 ------STTNVHPWLSS-----MVNYAQPIKFQGFDKAIEKNIAH------------------NM 577
                  |.::..|.::|     ::||.:..|.....:|:...:.:                  .:
Zfish   579 KKKPIVSDSDTEPEITSTNDKQVINYGKRRKTMRLSRALSDLVKYTKSVRVHDIETQAYMSSWQV 643

  Fly   578 SSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQSSDLP 642
            ||..||.....|:......|.:|:||:.|:||...|.||||:.||.||||||.:|:||:|:....
Zfish   644 SSLNESITNQILQLKPAQLVRFNQRQLLRVYPSNYRVDSSNFNPQPFWNAGCHLVALNYQTEGRM 708

  Fly   643 MQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKK----- 702
            :|||:.||..||.|||:|||..|.:.  .|:|..:.|:.|...:|..:|:|:||.|...|
Zfish   709 LQLNRAKFAANGNCGYVLKPKCMCKG--AFNPVLEDPLPGHRKSQLVLKIISGQQLPKPKDSMLG 771

  Fly   703 -----VGTYVEVDMFGLPSDTVKKEFRTRLVANNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEE 762
                 :..:|||::.|||.|..|::  ||:|.:||.||.: |:..|| .:.:|.:|::||.|::.
Zfish   772 DRGEIIDPFVEVEIIGLPIDCNKQQ--TRVVDDNGFNPTW-EETLVF-TLHMPQIALVRFMVWDH 832

  Fly   763 S--GK-ILGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPR 824
            .  |: .:|||.:....:..|||||.|.     .|....:||::.:                  .
Zfish   833 DPIGRDFIGQRTISFKSMMPGYRHVYLE-----GMEEASIFVHVAV------------------N 874

  Fly   825 GFAGAAKQQN--EQMKALGIEEQSGGAARDAGKAKEEEKKEPPLVFEPVTLESLRQEKGFQKVGK 887
            ...|..|..|  :|:....:.:.|....|.:              |.|..|         :|.||
Zfish   875 DITGKVKSSNAVKQLIQKNLMQTSEDGTRKS--------------FAPNFL---------RKSGK 916

  Fly   888 KQIKEL--------DTLRKKHAKERTSVQKTQNAAIDKLIKGKSKDDIR 928
            .|.|.:        |.|.::::.:.:|......:....|::|...:.::
Zfish   917 NQSKVVKVECYTKEDFLNRRYSDDVSSKDSRSTSQSASLLRGAQSEPLK 965

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
norpA	NP_001014720.1	PH_PLC_beta	17..149	CDD:270167	21/151 (14%)
		PLN02952	224..786	CDD:178538	200/620 (32%)
		EF-hand_like	227..318	CDD:286375	18/90 (20%)
		PI-PLCc_beta	318..653	CDD:176533	129/380 (34%)
		C2_PLC_like	689..806	CDD:175974	42/129 (33%)
		DUF1154	866..908	CDD:284131	11/49 (22%)
		Mer2	<887..1003	CDD:286200	8/50 (16%)
plch2b	XP_017213827.1	PH_PLC_eta	86..192	CDD:270170	18/128 (14%)
		EFh_PI-PLC	210..350	CDD:333715	34/157 (22%)
		EF-hand motif	210..239	CDD:320029	6/37 (16%)
		EF-hand motif	246..275	CDD:320029	7/28 (25%)
		EF-hand motif	280..308	CDD:320029	6/28 (21%)
		EF-hand motif	317..350	CDD:320029	11/32 (34%)
		PI-PLCc_GDPD_SF	362..719	CDD:326331	129/380 (34%)
		C2_PLC_like	749..873	CDD:175974	43/132 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0169
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D368239at2759
OrthoFinder	1	1.000	-	-		FOG0000126
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	5	4.820