DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment norpA and Plcb4

DIOPT Version :9

Sequence 1:	NP_001014720.1	Gene:	norpA / 31376	FlyBaseID:	FBgn0262738	Length:	1095	Species:	Drosophila melanogaster
Sequence 2:	XP_006235145.1	Gene:	Plcb4 / 25031	RGDID:	3345	Length:	1206	Species:	Rattus norvegicus

Alignment Length:	1196	Identity:	608/1196 - (50%)
Similarity:	798/1196 - (66%)	Gaps:	123/1196 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MTKKYEFDWIIPVPPELTTGCVFDRWFENEKETKENDFERDALFKVDEYGFFLYWKSEGRDGDVI 65
            |.|.|||:|...||..|..|.||||:     |.:...||.:.||||||:||||.|||||::|.|:
  Rat     1 MAKPYEFNWQKEVPSFLQEGAVFDRY-----EEESFVFEPNCLFKVDEFGFFLTWKSEGKEGQVL 60

  Fly    66 ELCQVSDIRAGGTPKDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKI 130
            |...::.||....||||||| ...:..|.:..:|:.|.|.:||.||.:||.:.:::..:....|.
  Rat    61 ECSLINSIRLAAIPKDPKIL-AALESVGKSENDLEGRILCVCSGTDLVNIGFTYMVAENPEITKQ 124

  Fly   131 WLDGIRKITHNVKANNICPMMCLRKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDA 195
            |::|:|.|.||.:|||:.||.||:||||:|::....||||||:::.:|||||||||:::..:|:.
  Rat   125 WVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFLTNTSGKIPVRSITRTFASGKTEKVIFQALKEL 189

  Fly   196 GLPDDKNATMTKEQFTFDKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPR 260
            |||..||..:....||::|||.|..|:|||.|||:||..|...|.|:::::|.:.|:|:.|||||
  Rat   190 GLPSGKNDEIEPAAFTYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPR 254

  Fly   261 MNEILYPLYEEKRCTEIINDYELDEEKKKNVQMSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPL 325
            :||||:|.|:.||..:||..||.|||.||...:|.|||.||||||||||||||||:.|.|||.||
  Rat   255 LNEILFPFYDAKRAMQIIEMYEPDEELKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPL 319

  Fly   326 AHYYINSSHNTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEIL 390
            |||:|:|||||||:|||.|||||||||||.|||||||||||||:|||||:|||:|||.|.||:||
  Rat   320 AHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDIL 384

  Fly   391 FKDCIQAIADCAFVSSEYPVILSFENHCNRAQQYKLAKYCDDFFGDLLLKEPLPDHPLDPGLPLP 455
            |||.||||.:.|||:|||||||||||||::.||||::|||:|.|||||||:.|..|||:||.|||
  Rat   385 FKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLP 449

  Fly   456 PPCKLKRKILIKNKRMKPEVEKVELELWLKGELKT-----------DDDPEEDASAGKPPEAAAA 509
            .|..|||||||||||:||||||.:||. ||..::.           ||:.||..||.:..||
  Rat   450 SPNDLKRKILIKNKRLKPEVEKKQLEA-LKSMMEAGESAAPASILEDDNEEEIESADQEEEA--- 510

  Fly   510 PAPAPEAAAAAEGAAEGGGGAEAEA-----------------------------AAANYSGSTTN 545
               .||.....|.:|:.....||.|                             |:..|.|:|||
  Rat   511 ---HPEYKFGNELSADDFSHKEAVANSVKKTSDDLEHENNKKGLVTVEDEQAWMASYKYVGATTN 572

  Fly   546 VHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSFAESAGMNYLKQSSIDFVNYNKRQMSRIYPK 610
            :||:||:|:|||||:|||||..|.|:||.:|||||.||.|:.|||..:|:|||||||||||||||
  Rat   573 IHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPK 637

  Fly   611 GTRADSSNYMPQVFWNAGCQMVSLNFQSSDLPMQLNQGKFEYNGGCGYLLKPDFMRRADKDFDPF 675
            |.|.||||||||:||||||||||||:|:.||.||||||||||||.||||||||||||.|:.||||
  Rat   638 GGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPF 702

  Fly   676 ADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVDMFGLPSDTVKKEFRTRLVANNGLNPVYNED 740
            ::.||||||||.|||:||:||||||||:||||||||:|||:||::||||||:|.||||||||||:
  Rat   703 SETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEE 767

  Fly   741 PFVFRKVVLPDLAVLRFGVYEESGKILGQRILPLDGLQAGYRHVSLRTEANFPMSLPMLFVNIEL 805
            .||||||:||||||||..||:::.|::||||||||||||||||:|||.|.|.|:|||.:|.||.|
  Rat   768 SFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVL 832

  Fly   806 KIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKALGIEEQ------SGGAARD-AGKAK------ 857
            |.||||||.|.:..||||:.|....:::.:||:|:|||..      |..:..| .|||.
  Rat   833 KTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANPAKANV 897

  Fly   858 -------------------EEEKKEPPLVFEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKE 903
                               :|.||...|: ..|.:|.|:|.|.:.|..|||.|||::|:||||||
  Rat   898 TPQSSSELRPTTTAALGSGQEAKKGIELI-PQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKE 961

  Fly   904 RTSVQKTQNAAIDKLI--------------------KG-------KSKDDIR-------NDANIK 934
            .:::||.....:||::                    ||       |.:.:|:       :.:.:|
  Rat   962 HSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEIKKETEIKIQTLTSDHKSKVK 1026

  Fly   935 NSINDQTKQWTDMIARHRKEEWDMLRQHVQDSQDAMKALMLTVQAAQIKQLEDRHARDIKDLNAK 999
            ..:...||:|::||..|..||.::...|:....:.::.|::.....|.:||:..|.|:.|::.|.
  Rat  1027 EIVAQHTKEWSEMINTHSAEEQEIRDLHLSQQCELLRKLLINAHEQQTQQLKLSHDRESKEMRAH 1091

  Fly  1000 QAKMSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQIGVKQGRAMEKLKLAHSKQI 1064
            |||:|.:.:|.:..||::|.|.|::||:||...:|.|:|:||:|::.:||.:.|::||....:.:
  Rat  1092 QAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHL 1156

  Fly  1065 EEFSTDVQKLMDMYKIEEEAYKTQGK 1090
            |......::|:   |......:|||:
  Rat  1157 EFLEKQNEQLL---KSCHAVSQTQGE 1179

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
norpA	NP_001014720.1	PH_PLC_beta	17..149	CDD:270167	56/131 (43%)
		PLN02952	224..786	CDD:178538	389/601 (65%)
		EF-hand_like	227..318	CDD:286375	53/90 (59%)
		PI-PLCc_beta	318..653	CDD:176533	230/374 (61%)
		C2_PLC_like	689..806	CDD:175974	87/116 (75%)
		DUF1154	866..908	CDD:284131	20/41 (49%)
		Mer2	<887..1003	CDD:286200	41/149 (28%)
Plcb4	XP_006235145.1	PH_PLC_beta	17..143	CDD:270167	56/131 (43%)
		EFh_PI-PLCbeta4	148..300	CDD:320041	78/151 (52%)
		EF-hand motif	148..177	CDD:320041	15/28 (54%)
		EF-hand motif	183..211	CDD:320041	10/27 (37%)
		EF-hand motif	221..250	CDD:320041	11/28 (39%)
		EF-hand motif	267..300	CDD:320041	19/32 (59%)
		PI-PLCc_beta	312..680	CDD:176533	230/374 (61%)
		C2_PLC_like	714..830	CDD:175974	86/115 (75%)
		PTZ00121	<859..1206	CDD:173412	91/325 (28%)
		DUF1154	926..967	CDD:399553	19/41 (46%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353421
Domainoid	1	1.000	248	1.000	Domainoid score	I2067
eggNOG	1	0.900	-	-		E1_KOG0169
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8471
Inparanoid	1	1.050	1164	1.000	Inparanoid score	I193
OMA	1	1.010	-	-		QHG45247
OrthoDB	1	1.010	-	-		D368239at2759
OrthoFinder	1	1.000	-	-		FOG0000126
OrthoInspector	1	1.000	-	-		oto97750
orthoMCL	1	0.900	-	-		OOG6_102409
Panther	1	1.100	-	-	LDO	PTHR10336
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X5343
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.810