Protein Alignment norpA and Plcb1

DIOPT Version :10

• Sequence 1: NP_525069.2
• Gene: norpA / 31376
• FlyBaseID: FBgn0262738
• Length: 1095
• Species: Drosophila melanogaster
• Sequence 2: NP_001139302.1
• Gene: Plcb1 / 18795
• MGIID: 97613
• Length: 1216
• Species: Mus musculus
• Alignment Length: 1157
• Identity: 432/1157 - (37%)
• Similarity: 646/1157 - (55%)
• Gaps: 127/1157 - (10%)

  Fly    16 ELTTGCVFDRWFENEKETKEND---FERDALFKVDEYGFFLYWKSEGRDGDVIELCQVSDIRAG- 76
            :|...||.|...:..|..|.:|   .....:.:.|..|||.||..:.::.::::|..|.|.|.|
Mouse    12 QLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGK 76

  Fly    77 --GTPKDPKILDKVTKKNGTNIPELDKRSLTICSNTDYINITYHHVICPDAATAKSWQKNLRLIT 139
              ..|||||:.:.:   :..||..|::|.:|:....|.:||::.:::......||.|...:..:.
Mouse    77 HAKAPKDPKLRELL---DVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLA 138

  Fly   140 HNNRATNVCPRVNLMKHWMRLSYCVEKSGKIPVKTLAKTFASGKTEKLVYTCIKDAGLPDDKNAT 204
            .|..|.|:.....|.|.:.:|...|...|:||:|.:.:.|::.:  |.|.|.::...||..:|.:
Mouse   139 TNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADR--KRVETALEACSLPSSRNDS 201

  Fly   205 MTKEQFTFDKFYALYHKVCPRNDIEELFTSITKGKQDFISLEQFIQFMNDKQRDPRMNEILYPLY 269
            :.:|.||.|.:....:.:|||.:|:.:|:......:.:::::|.:.|:|.||||||:||||||..
Mouse   202 IPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPL 266

  Fly   270 EEKRCTEIINDYELDEEKKKNVQMSLDGFKRYLMSDENAPVFLDRLDFYMEMDQPLAHYYINSSH 334
            ::::...:|..||.:....|..|||:|||.|||..:||..|..::||...:|.|||:||:|||||
Mouse   267 KQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSH 331

  Fly   335 NTYLSGRQIGGKSSVEMYRQTLLAGCRCVELDCWNGKGEDEEPIVTHGHAYCTEILFKDCIQAIA 399
            ||||:..|:.|.||||||||.||:||||||||||.|:..:|||::|||....|||.||:.|:|||
Mouse   332 NTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIA 396

  Fly   400 DCAFVSSEYPVILSFENHCNR-AQQYKLAKYCDDFFGDLLLKEPLPDHPLDPGLPLPPPCKLKRK 463
            :|||.:|.:|::||||||.:. .||.|:|:||...|||.||.|||..:||:.|:|||.|..|..|
Mouse   397 ECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYK 461

  Fly   464 ILIKNKRMKPEVEKVELELWLKGELKTDDDPEEDASAGKPPEAAAAPAPAPEAAAAAEGAAEGGG 528
            ||:|||:                  |:....|   .:||...:..|.....::::..|.::.|.|
Mouse   462 ILVKNKK------------------KSHKSSE---GSGKKKLSEQASNTYSDSSSVFEPSSPGAG 505

  Fly   529 GAEAE-------------AAAANYSGSTTNVHPWLSSMVNYAQPIKFQGFDKAIEKNIAHNMSSF 580
            .|:.|             :.....:||.......:|::|||.||:||:.|:.:.::|.:..||||
Mouse   506 EADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSF 570

  Fly   581 AESAGMNYLKQSSIDFVNYNKRQMSRIYPKGTRADSSNYMPQVFWNAGCQMVSLNFQSSDLPMQL 645
            .|:.|:..|.:|.::||.|||.|:|||||||||.||||||||:|||||||||:||||:.||.||:
Mouse   571 VETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQI 635

  Fly   646 NQGKFEYNGGCGYLLKPDFMRRADKDFDPFADAPVDGVIAAQCSVKVIAGQFLSDKKVGTYVEVD 710
            |.|.:||||..||.|||:||||.||.||||.:..|||::|...|||:|:||||||||||||||||
Mouse   636 NMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD 700

  Fly   711 MFGLPSDTVKKEFRTRLVANNGLNPVYNEDPFVFRKVVLPDLAVLRFGVYEESGKILGQRILPLD 775
            |||||.||.:|.|:|:....|.:|||:.|:|.||:|||||.||.||...|||.||.:|.||||:.
Mouse   701 MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQ 765

  Fly   776 GLQAGYRHVSLRTEANFPMSLPMLFVNIELKIYVPDGFEDFMAMLSDPRGFAGAAKQQNEQMKAL 840
            .::.||.::.||.|.|.|::||.:||.||:|.||||.:.|.:..||:|..:....:|:.:|:.||
Mouse   766 AIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAAL 830

  Fly   841 GIE-EQSGGAARDAGKAKEEEKKE-------------------PP-------------------- 865
            .:| |:......|.|:...|...|                   ||
Mouse   831 TLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTE 895

  Fly   866 -LV------FEPVTLESLRQEKGFQKVGKKQIKELDTLRKKHAKERTSVQKTQNAAIDKLIKGKS 923
             |:      .|..|:|.|:|:|.|.|:.||..||:..|.|:|.|:.|.:.|......:::    .
Mouse   896 DLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEI----Q 956

  Fly   924 KDDIRNDANIKNSINDQTKQWTDMIARHRKE---EWDM------LRQHVQDSQDAMKALMLTVQA 979
            .|.:|..|.::.|....:|:.::..:.....   |.|:      :.|.:.|.:|..:..:|.::.
Mouse   957 NDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQ 1021

  Fly   980 AQIKQLEDRHARDIKDLNAKQAKMSADTAKEVQNDKTLKTKNEKDRRLREKRQNNVKRFMEEKKQ 1044
            .|....:.:....||.|..|    ..|.|:|.||::..|.|     .:.||.:..:|:.|::|:|
Mouse  1022 EQYYSEKYQKREHIKLLIQK----LTDVAEECQNNQLKKLK-----EICEKEKKELKKKMDKKRQ 1077

  Fly  1045 IGVKQGRAMEKLKLAHSKQIEEFSTD-----VQKLMDMYK-IEEEAYKTQGK 1090
            ..:.:.::.:|      .|:||..|:     :|:::...| :||...|.|.|
Mouse  1078 EKITEAKSKDK------SQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEK 1123

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
norpA	NP_525069.2	PH_PLC_beta	17..149	CDD:270167	37/137 (27%)
		EFh_NorpA_like	154..306	CDD:320042	51/151 (34%)
		EF-hand motif	154..183	CDD:320042	8/28 (29%)
		EF-hand motif	189..218	CDD:320042	8/28 (29%)
		EF-hand motif	227..256	CDD:320042	5/28 (18%)
		EF-hand motif	273..306	CDD:320042	13/32 (41%)
		PI-PLCc_beta	318..653	CDD:176533	165/348 (47%)
		C2_PLC_like	689..806	CDD:175974	70/116 (60%)
		DUF1154	865..909	CDD:461969	19/70 (27%)
		PLC-beta_C	952..1073	CDD:462571	29/134 (22%)
Plcb1	NP_001139302.1	PH_PLC_beta	22..148	CDD:270167	33/128 (26%)
		EFh_PI-PLCbeta1	153..303	CDD:320038	51/151 (34%)
		EF-hand motif	153..182	CDD:320038	8/28 (29%)
		EF-hand motif	186..215	CDD:320038	8/28 (29%)
		EF-hand motif	224..253	CDD:320038	5/28 (18%)
		EF-hand motif	270..303	CDD:320038	13/32 (41%)
		PI-PLCc_beta	316..643	CDD:176533	165/347 (48%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	469..534		12/67 (18%)
		C2_PLC_like	677..796	CDD:175974	70/118 (59%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	834..891		7/56 (13%)
		DUF1154	903..939	CDD:461969	15/35 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	967..989		2/21 (10%)
		PLC-beta_C	1003..1170	CDD:462571	34/136 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1072..1095		7/28 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1173..1216