back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment N and Notch1

DIOPT Version :9

Sequence 1:	NP_001245510.1	Gene:	N / 31293	FlyBaseID:	FBgn0004647	Length:	2703	Species:	Drosophila melanogaster
Sequence 2:	NP_032740.3	Gene:	Notch1 / 18128	MGIID:	97363	Length:	2531	Species:	Mus musculus

Alignment Length:	2741	Identity:	1223/2741 - (44%)
Similarity:	1571/2741 - (57%)	Gaps:	326/2741 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    35 PLLLLTLAFANLPN-TVRGTDTALVAASCTSVGCQNGGTCVTQLNGKTYCACDSHYVGDYCEHRN 98
            |||.|||    ||. ..||     :..|..|..|.|||.|.. .||...|.|...:||..|:..|
Mouse     7 PLLCLTL----LPALAARG-----LRCSQPSGTCLNGGRCEV-ANGTEACVCSGAFVGQRCQDSN 61

  Fly    99 PCNSMRCQNGGTCQVTFRNGRPGISCKCPLGFDESLCEIAVPNACDHVTCLNGGTCQLKTLEEYT 163
            ||.|..|:|.|||.|....|....:|.|||||...||...:.|||....|.|||||.|.||.||.
Mouse    62 PCLSTPCKNAGTCHVVDHGGTVDYACSCPLGFSGPLCLTPLDNACLANPCRNGGTCDLLTLTEYK 126

  Fly   164 CACANGYTGERCETKNLCASSPCRNGATCTALAGSSSFTCSCPPGFTGDTCSYDIEECQSNP--C 226
            |.|..|::|:.|:..:.|||:||.||..|  |...||:.|.|||||.|.||..|:.||..||  |
Mouse   127 CRCPPGWSGKSCQQADPCASNPCANGGQC--LPFESSYICRCPPGFHGPTCRQDVNECSQNPGLC 189

  Fly   227 KYGGTCVNTHGSYQCMCPTGYTGKDCDTKYKPCSPSPCQNGGICRSNG-LSYECKCPKGFEGKNC 290
            ::||||.|..|||:|.|...:||..|:..|.|||||||||||.||..| .::||.|..||.|:||
Mouse   190 RHGGTCHNEIGSYRCACRATHTGPHCELPYVPCSPSPCQNGGTCRPTGDTTHECACLPGFAGQNC 254

  Fly   291 EQNYDDCLGHLCQNGGTCIDGISDYTCRCPPNFTGRFCQDDVDECAQRDHPVCQNGATCTNTHGS 355
            |:|.|||.|:.|:|||.|:||::.|.|||||.:||::|.:||||| |.....||||.||.||||.
Mouse   255 EENVDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDEC-QLMPNACQNGGTCHNTHGG 318

  Fly   356 YSCICVNGWAGLDCSNNTDDCKQAACFYGATCIDGVGSFYCQCTKGKTGLLCHLDDACTSNPCHA 420
            |:|:|||||.|.|||.|.|||..||||.||||.|.|.||||:|..|:|||||||:|||.||||:.
Mouse   319 YNCVCVNGWTGEDCSENIDDCASAACFQGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNE 383

  Fly   421 DAICDTSPINGSYACSCATGYKGVDCSEDIDECDQG-SPCEHNGICVNTPGSYRCNCSQGFTGPR 484
            .:.|||:|:||...|:|.:||.|..||:|:|||..| :||||.|.|:||.||:.|.|.||:||||
Mouse   384 GSNCDTNPVNGKAICTCPSGYTGPACSQDVDECALGANPCEHAGKCLNTLGSFECQCLQGYTGPR 448

  Fly   485 CETNINECESHPCQNEGSCLDDPGTFRCVCMPGFTGTQCEIDIDECQSNPCLNDGTCHDKINGFK 549
            ||.::|||.|:||||:.:|||..|.|:|:||||:.|..|||:.|||.|:|||::|.|.||||.|:
Mouse   449 CEIDVNECISNPCQNDATCLDQIGEFQCICMPGYEGVYCEINTDECASSPCLHNGHCMDKINEFQ 513

  Fly   550 CSCALGFTGARCQINIDDCQSQPCRNRGICHDSIAGYSCECPPGYTGTSCEININDCDSNPCHRG 614
            |.|..||.|..||.::|:|.|.||:|...|.|....|:|.|..|||||.||::|::||.:|||.|
Mouse   514 CQCPKGFNGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYG 578

  Fly   615 KCIDDVNSFKCLCDPGYTGYICQKQINECESNPCQFDGHCQDRVGSYYCQCQAGTSGKNCEVNVN 679
            .|.|.|.:|.|||.|||||:.|:..||||.|.||:..|.||||..||.|.|..||:|.|||:|::
Mouse   579 SCKDGVATFTCLCQPGYTGHHCETNINECHSQPCRHGGTCQDRDNSYLCLCLKGTTGPNCEINLD 643

  Fly   680 ECHSNPCNNGATCIDGINSYKCQCVPGFTGQHCEKNVDECISSPCANNGVCIDQVNGYKCECPRG 744
            :|.||||::| ||:|.|:.|:|.|.||:||..|..|:|||..|||.|.|.|.|.:.|:.|.||.|
Mouse   644 DCASNPCDSG-TCLDKIDGYECACEPGYTGSMCNVNIDECAGSPCHNGGTCEDGIAGFTCRCPEG 707

  Fly   745 FYDAHCLSDVDECASNPCVNEGRCEDGINEFICHCPPGYTGKRCELDIDECSSNPCQHGGTCYDK 809
            ::|..|||:|:||.||||:: |.|.||:|.:.|.|.||::|..|:::.:||.||||.:||||.|.
Mouse   708 YHDPTCLSEVNECNSNPCIH-GACRDGLNGYKCDCAPGWSGTNCDINNNECESNPCVNGGTCKDM 771

  Fly   810 LNAFSCQCMPGYTGQKCETNIDDCVTNPCGNGGTCIDKVNGYKCVCKVPFTGRDCESKMDPCASN 874
            .:.:.|.|..|::|..|:|||::|.:|||.|.|||||.|.||||.|.:|:||..||..:.|||::
Mouse   772 TSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCPLPYTGATCEVVLAPCATS 836

  Fly   875 RCKNEAKCTPSSNFLDFSCTCKLGYTGRYCDEDIDECSLSSPCRNGASCLNVPGSYRCLCTKGYE 939
            .|||...|..|.::..|||.|..|:.|:.|:.||:|| :.||||:||||.|..|||||||..||.
Mouse   837 PCKNSGVCKESEDYESFSCVCPTGWQGQTCEVDINEC-VKSPCRHGASCQNTNGSYRCLCQAGYT 900

  Fly   940 GRDCAINTDDCASFPCQNGGTCLDGIGDYSCLCVDGFDGKHCETDINECLSQPCQNGATCSQYVN 1004
            ||:|..:.|||...||.|||:|.|||....|.|:.||.|..||.|||||.|.||||||.|:..|:
Mouse   901 GRNCESDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFQGAFCEEDINECASNPCQNGANCTDCVD 965

  Fly  1005 SYTCTCPLGFSGINCQTNDEDCTESSCLNGGSCIDGINGYNCSCLAGYSGANCQYKLNKCDSNPC 1069
            |||||||:||:||:|:.|..|||||||.|||:|:||||.:.|.|..|::|:.|||.:|:|||.||
Mouse   966 SYTCTCPVGFNGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQYDVNECDSRPC 1030

  Fly  1070 LNGATCHEQNNEYTCHCPSGFTGKQCSEYVDWCGQSPCENGATCSQMKHQFSCKCSAGWTGKLCD 1134
            |:|.||.:....|.|.||.|:||..|...|.||..:||:||..|.|...|:.|:|.:||||..||
Mouse  1031 LHGGTCQDSYGTYKCTCPQGYTGLNCQNLVRWCDSAPCKNGGRCWQTNTQYHCECRSGWTGVNCD 1095

  Fly  1135 VQTISCQDAADRKGLSLRQLC-NNGTCKDYGNSHVCYCSQGYAGSYCQKEIDECQSQPCQNGGTC 1198
            |.::||:.||.::|:.:..|| :.|.|.|.|:.|.|:|..||.||||:.|:|||...|||||.||
Mouse  1096 VLSVSCEVAAQKRGIDVTLLCQHGGLCVDEGDKHYCHCQAGYTGSYCEDEVDECSPNPCQNGATC 1160

  Fly  1199 RDLIGAYECQCRQGFQGQNCELNIDDCAPNPCQNGGTCHDRVMNFSCSCPPGTMGIICEINKDDC 1263
            .|.:|.:.|:|..|:.|.||...|::|...||||||||.|...::.||||.||.|:.||||.|||
Mouse  1161 TDYLGGFSCKCVAGYHGSNCSEEINECLSQPCQNGGTCIDLTNSYKCSCPRGTQGVHCEINVDDC 1225

  Fly  1264 KP--------GACHNNGSCIDRVGGFECVCQPGFVGARCEGDINECLSNPCSNAGTLDCVQLVNN 1320
            .|        ..|.|||:|:|:|||:.|.|.|||||.|||||:||||||||...||.:|||.||:
Mouse  1226 HPPLDPASRSPKCFNNGTCVDQVGGYTCTCPPGFVGERCEGDVNECLSNPCDPRGTQNCVQRVND 1290

  Fly  1321 YHCNCRPGHMGRHCEHKVDFCAQSPCQNGGNCNIRQS---GHHCICNNGFYGKNCELSGQDCDSN 1382
            :||.||.||.||.||..::.|...||:|||.|.:..:   |..|.|..||.|..||...:.|.|.
Mouse  1291 FHCECRAGHTGRRCESVINGCRGKPCKNGGVCAVASNTARGFICRCPAGFEGATCENDARTCGSL 1355

  Fly  1383 PCRVGNCVVADEGFGYR---CECPRGTLGEHCEIDTLDEC-SPNPCAQGAACEDLLGD--YECLC 1441
            .|..|...::    |.|   |.|.....|..|:......| ..|||.....||....:  |.|||
Mouse  1356 RCLNGGTCIS----GPRSPTCLCLGSFTGPECQFPASSPCVGSNPCYNQGTCEPTSENPFYRCLC 1416

  Fly  1442 PSKWKGKRCDIYDANYPGWNGGSGSGNDRYAADLEQQRAMCDKRGCTEKQGNGICDSDCNTYACN 1506
            |:|:.|..|.|.|.::.|     |:|.|.....:|:   .|:...|....||.:|:..||.:||.
Mouse  1417 PAKFNGLLCHILDYSFTG-----GAGRDIPPPQIEE---ACELPECQVDAGNKVCNLQCNNHACG 1473

  Fly  1507 FDGNDCSLGIN-PWANCTAN-ECWNKFKNGKCNEECNNAACHYDGHDCERKLKSCDSLFDAYCQK 1569
            :||.||||..| ||.|||.: :||..|.:|.|:.:||:|.|.:||.||:.....|:.|:|.||:.
Mouse  1474 WDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNPLYDQYCKD 1538

  Fly  1570 HYGDGFCDYGCNNAECSWDGLDCENKTQSPVLAEGAMSVVMLMNVEAFREIQAQFLRNMSHMLRT 1634
            |:.||.||.|||:|||.||||||....... ||.|.:.:|:|:..:..|.....|||.:||:|.|
Mouse  1539 HFSDGHCDQGCNSAECEWDGLDCAEHVPER-LAAGTLVLVVLLPPDQLRNNSFHFLRELSHVLHT 1602

  Fly  1635 TVRLKKDALGHDII-------------------INWKDNVRVPEIEDTDFARKNKILYTQQVHQT 1680
            .|..|:||.|..:|                   :.|..:..:|   .|...|:.:.|....:.
Mouse  1603 NVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLP---GTSGGRQRRELDPMDIR-- 1662

  Fly  1681 GIQIYLEIDNRKC----TECFTHAVEAAEFLAATAAKHQLRNDFQIHSVRGIKNPGDEDNGEPPA 1741
            |..:|||||||:|    ::||..|.:.|.||.|.|:...|...::|.:|:       .:..|||.
Mouse  1663 GSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYKIEAVK-------SEPVEPPL 1720

  Fly  1742 NVKYVITGIILVIIALAFF---GMVLSTQRKRAHGVTWFPEGFRAPAAVMSRRRRDPHGQEMRNL 1803
            ..:..:..:......|.||   |::||.:|:|.||..||||||:...| ..::||:|.|::...|
Mouse  1721 PSQLHLMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEA-SKKKRREPLGEDSVGL 1784

  Fly  1804 NKQVAMQSQG--VGQPGAHWSDDESDMPLPKRQRSDPVSGVGLGNNGGYASDHTMVSEYEEADQR 1866
             |.:...|.|  :......|.|:  |:...|.:..:||....|       ||.|        |.|
Mouse  1785 -KPLKNASDGALMDDNQNEWGDE--DLETKKFRFEEPVVLPDL-------SDQT--------DHR 1831

  Fly  1867 VWSQAHLDVVDVR---AIMTPPAHQ-DGGKHDVDARGPCGLTPLMIAAVRGGGLDTGEDIENNED 1927
            .|:|.|||..|:|   ...|||..: |....||:.|||.|.||||||:..||||:||.. |..||
Mouse  1832 QWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNS-EEEED 1895

  Fly  1928 STAQVISDLLAQGAELNATMDKTGETSLHLAARFARADAAKRLLDAGADANCQDNTGRTPLHAAV 1992
            :.| ||||.:.|||.|:...|:||||:||||||::|:|||||||:|.||||.|||.|||||||||
Mouse  1896 APA-VISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAV 1959

  Fly  1993 AADAMGVFQILLRNRATNLNARMHDGTTPLILAARLAIEGMVEDLITADADINAADNSGKTALHW 2057
            :|||.||||||||||||:|:|||||||||||||||||:|||:||||.:.||:||.|:.||:||||
Mouse  1960 SADAQGVFQILLRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHW 2024

  Fly  2058 AAAVNNTEAVNILLMHHANRDAQDDKDETPLFLAAREGSYEACKALLDNFANREITDHMDRLPRD 2122
            ||||||.:|..:||.:.||:|.|::|:||||||||||||||..|.|||:||||:|||||||||||
Mouse  2025 AAAVNNVDAAVVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRD 2089

  Fly  2123 VASERLHHDIVRLLDEH-VPRSPQMLSMTPQAMIGSPPPGQQQPQLITQPTVISAGNGGNNGNGN 2186
            :|.||:||||||||||: :.||||:..   .|:.|:|         ...||:.|......|....
Mouse  2090 IAQERMHHDIVRLLDEYNLVRSPQLHG---TALGGTP---------TLSPTLCSPNGYLGNLKSA 2142

  Fly  2187 ASGKQSNQTAKQKAA---KKAKLIEGSPDNGLDATGSLRRKASSKKTSAASKKAANLNGLNPGQL 2248
            ..||::.:.:.:..|   |:||.::.......|..|.| ..:||..:...|.::.:
Mouse  2143 TQGKKARKPSTKGLACGSKEAKDLKARRKKSQDGKGCL-LDSSSMLSPVDSLESPH--------- 2197

  Fly  2249 TGGVSGVPGVPPTNSAAQAAAAAAAAVAAMSHELEGSP---VGVGMGGNLPSPYDTSSMYSNAMA 2310
             |.:|.|...|...|..|.:.:     ..:|| |.|.|   :|:.              :.|..|
Mouse  2198 -GYLSDVASPPLLPSPFQQSPS-----MPLSH-LPGMPDTHLGIS--------------HLNVAA 2241

  Fly  2311 AP----LANGNPNTGAKQPP--SYEDCIKNAQSMQSLQGNGLDMIKLDNYAYSMGSPFQQELLNG 2369
            .|    ||.|:.......||  |:.....:|.::.|..|.|.       ..:::|:|..   |||
Mouse  2242 KPEMAALAGGSRLAFEPPPPRLSHLPVASSASTVLSTNGTGA-------MNFTVGAPAS---LNG 2296

  Fly  2370 Q--GLGMNGNG----QRNGVGPGVLPGGLCGMGGLSGAGNGNSHEQGLSPPYSNQSPPHSVQSSL 2428
            |  .|....||    |.|.:.|||.||.|            ::...||.  :|...|.||..|:.
Mouse  2297 QCEWLPRLQNGMVPSQYNPLRPGVTPGTL------------STQAAGLQ--HSMMGPLHSSLSTN 2347

  Fly  2429 ALSPHAYLGSPSPAKSRPSLPTSPTHIQAMRHATQQKQFGGSNLNSLLGGANGGGVVGGGGGGGG 2493
            .|||..|.|.|:     ..|.|.|..:|     |||.|
Mouse  2348 TLSPIIYQGLPN-----TRLATQPHLVQ-----TQQVQ--------------------------- 2375

  Fly  2494 GVGQGPQNSPVSLGIISPTGSDMGIMLAPPQSSKNSAIMQTISPQQQQQQQQQQQQQHQQQQQQQ 2558
                 |||.                                   |.|.|..|...|.|
Mouse  2376 -----PQNL-----------------------------------QLQPQNLQPPSQPH------- 2393

  Fly  2559 QQQQQQQQQQLGGLEFGSAGLDLNGFCGSPDSFHSGQMNPPSIQSSMSGSSPSTNMLS------- 2616
                         |...||.   ||..|.  ||.||:.:...:|.....|.|...:|.
Mouse  2394 -------------LSVSSAA---NGHLGR--SFLSGEPSQADVQPLGPSSLPVHTILPQESQALP 2440

  Fly  2617 ---PSSQHNQQAFYQYLTPSSQHSGG-----HTPQHLVQTLDSYP--TPSPESPGHWSSSSPRSN 2671
               |||........|:|||.||||..     :||.|.:| :..:|  |||||||..||||||.||
Mouse  2441 TSLPSSMVPPMTTTQFLTPPSQHSYSSSPVDNTPSHQLQ-VPEHPFLTPSPESPDQWSSSSPHSN 2504

  Fly  2672 -SDWSEGVQSP 2681
             ||||||:.||
Mouse  2505 ISDWSEGISSP 2515

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 591.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.