Protein Alignment Pi4KIIIalpha and TRA1

DIOPT Version :9

• Sequence 1: NP_001259191.1
• Gene: Pi4KIIIalpha / 31247
• FlyBaseID: FBgn0267350
• Length: 2154
• Species: Drosophila melanogaster
• Sequence 2: NP_011967.1
• Gene: TRA1 / 856499
• SGDID: S000001141
• Length: 3744
• Species: Saccharomyces cerevisiae
• Alignment Length: 2223
• Identity: 400/2223 - (17%)
• Similarity: 652/2223 - (29%)
• Gaps: 936/2223 - (42%)

  Fly   330 IFAVSKKLLTKDTLEHLD------EQASDIF--------------------------------SL 356
            |:..|..:|..|.|:|.|      .|.|.||                                |.
Yeast  1841 IWKNSNAILAYDVLDHHDLFRFELLQLSAIFIKADPEIIAEIKKDIIKFCWNFIKLEDTLIKQSA 1905

  Fly   357 HQIKGYCYKSFS---ETLNLVLVTLLRE-------LLQHQVDLPTPF---------TKDVQ-EFV 401
            :.:..|....|.   :.:..|.|.|||.       |::..:|:.||.         |.|.. .:|
Yeast  1906 YLVTSYFISKFDFPIKVVTQVFVALLRSSHVEARYLVKQSLDVLTPVLHERMNAAGTPDTWINWV 1970

  Fly   402 KRLFLNGQTELQNKQQDQERERREENGIAVVNKYKV---NVMANAACVDLLVWAIRDETVDVDYN 463
            ||:.:...:...|                ::.::.:   ::..|:.  ||.:           .|
Yeast  1971 KRVMVENSSSQNN----------------ILYQFLISHPDLFFNSR--DLFI-----------SN 2006

  Fly   464 VPSLQNGLQFLLKKEADKLCGRLSQKLNLELSHKIVMDHMPLLMV----CLEGLGKLAHKFPNIA 524
            :....|.:.|:....:|              ||.:.:|...|::.    .||        ..|:.
Yeast  2007 IIHHMNKITFMSNSNSD--------------SHTLAIDLASLILYWENKTLE--------ITNVN 2049

  Fly   525 GTSISYLRDFLVAPSPILGKLHDHAMQSLAQQKKEKELTPFKIAVQHSDSRTAVVIYGDNQKPPG 589
            .|......|.:::.|                   :.::.|.:     :|: ||:::..:|..|
Yeast  2050 NTKTDSDGDVVMSDS-------------------KSDINPVE-----ADT-TAIIVDANNNSP-- 2087

  Fly   590 SGTGRSGHAAFESLRDAAIENLSIALRAAHTLDQFCVPALVANV--SNRLFTAEKSGSESQGECH 652
                                 :|:.||.|      |...|:..|  ||......:.|
Yeast  2088 ---------------------ISLHLREA------CTAFLIRYVCASNHRAIETELG-------- 2117

  Fly   653 KSNLVSLNIIVMLGHVAVALKDTSKTTQNILQFFIQRFC---KVPSE------QNALIVDQLGCM 708
               |.::||:..|      :.|...|..|:...:.::|.   .:.||      .|||  |.|...
Yeast  2118 ---LRAINILSEL------ISDKHWTNVNVKLVYFEKFLIFQDLDSENILYYCMNAL--DVLYVF 2171

  Fly   709 IISQCETHVFDEIMKMFSRVTVQSASLAYTSDPEHRKQFHHVSDAVVNALGNI--AANIQGDAEM 771
            ..::.:..:.:.:      .|:|:. |......:|    |.|.:|:...|..|  |...||.:.:
Yeast  2172 FKNKTKEWIMENL------PTIQNL-LEKCIKSDH----HDVQEALQKVLQVIMKAIKAQGVSVI 2225

  Fly   772 L--ELLGKLLELFVQIGLDGERSYDNTPGAQKASS-RAGNLGMLIPVIAVLVRRLP----PIKNP 829
            :  |..||   .|:|: |....:.|    .|:.|| .||     :.:..||....|    |:..|
Yeast  2226 IEEESPGK---TFIQM-LTSVITQD----LQETSSVTAG-----VTLAWVLFMNFPDNIVPLLTP 2277

  Fly   830 RQRLHKLFKDFWAYCVVMGFTNARLWPADWYQGVQQIAAKSPLLISQTAHKSDMRELN------- 887
                  |.|.|...|                        |..|.|||......:.|..
Yeast  2278 ------LMKTFSKLC------------------------KDHLSISQPKDAMALEEARITTKLLE 2312

  Fly   888 ---YTLAIK----SDSVNELRSQILVLLEHSSDNVATAINKLSFAQCTYLLSVYWL--------- 936
               |.|::|    .||.....|.:.:|::||.|.        :|.:....:|..|:
Yeast  2313 KVLYILSLKVSLLGDSRRPFLSTVALLIDHSMDQ--------NFLRKIVNMSRSWIFNTEIFPTV 2369

  Fly   937 --------EMLRVENADEPSLEPIMSYLCDTALQRDKTGIWQCVKCVADQVFEKFRNVLYAHDEI 993
                    :||..|...||||..:               .::.|..:.||  |.|.|.     ||
Yeast  2370 KEKAAILTKMLAFEIRGEPSLSKL---------------FYEIVLKLFDQ--EHFNNT-----EI 2412

  Fly   994 ------------REKVLESQATLLLVYFNHIHKPIQ-----MVADQYLSFLVDRFPHLLWNRRVL 1041
                        |.:.:..:...:.:..|.:.:.|:     ::.||...|:.| :|
Yeast  2413 TVRMEQPFLVGTRVEDIGIRKRFMTILDNSLERDIKERLYYVIRDQNWEFIAD-YP--------- 2467

  Fly  1042 WCMLDILQLLAYSLSLDPNEETPTLRVVSTPYTLQLMDSLP--------ARELRLKDFAD----R 1094
            | :...||||..|.:.:.......:..:|.|..||  :.||        ..:|.|.:|..    .
Yeast  2468 W-LNQALQLLYGSFNREKELSLKNIYCLSPPSILQ--EYLPENAEMVTEVNDLELSNFVKGHIAS 2529

  Fly  1095 CQGIVN-----------EAMKWAPRST-RSHLQEYPNQIPTPVLAHHSGLALAFDSVVSSSAQHT 1147
            .||:..           |.....|::. |:.:..:|....:.......|...:..::: |...||
Yeast  2530 MQGLCRIISSDFIDSLIEIFYQDPKAIHRAWVTLFPQVYKSIPKNEKYGFVRSIITLL-SKPYHT 2593

  Fly  1148 GTMSKRPSCVNS--DT----------PRFVSVLCL------------------------------ 1170
            ..:|.|.:.:|.  |:          |..|..|.:
Yeast  2594 RQISSRTNVINMLLDSISKIESLELPPHLVKYLAISYNAWYQSINILESIQSNTSIDNTKIIEAN 2658

  Fly  1171 -----------------------RSKYAGEISGLLSVLSEKDKAGLADR---------------- 1196
                                   |:||.....||     ..::.||.|:
Yeast  2659 EDALLELYVNLQEEDMFYGLWRRRAKYTETNIGL-----SYEQIGLWDKAQQLYEVAQVKARSGA 2718

  Fly  1197 ---------LVSDVWEACAEKSD--------ARHRGALWRATAYLIICSEISRKLLHAVASSQLE 1244
                     |..|.|..||||..        |:|.|    .|..|:.|.       ..||....:
Yeast  2719 LPYSQSEYALWEDNWIQCAEKLQHWDVLTELAKHEG----FTDLLLECG-------WRVADWNSD 2772

  Fly  1245 LFTESAMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFAWETEVTHPLAAYEGCKL 1309
               ..|:|.:|:....|.|.|:.:...|:     |.|...|.|.|    :.||..  ...||.:|
Yeast  2773 ---RDALEQSVKSVMDVPTPRRQMFKTFL-----ALQNFAESRKG----DQEVRK--LCDEGIQL 2823

  Fly  1310 -----VSKPILIAPHLIWL-----QLLSEMVDTAKYCN-----------------------RDK- 1340
                 ||.||...|...||     |.:..:..|..|.|                       ||:
Yeast  2824 SLIKWVSLPIRYTPAHKWLLHGFQQYMEFLEATQIYANLHTTTVQNLDSKAQEIKRILQAWRDRL 2888

  Fly  1341 ------VEMFCLLL----HR----------CLPVLKSSKQNRQVST-----------VGCRFKLL 1374
                  |.|:..|:    |.          .:|.|:.|..|..::|           |..||..:
Yeast  2889 PNTWDDVNMWNDLVTWRQHAFQVINNAYLPLIPALQQSNSNSNINTHAYRGYHEIAWVINRFAHV 2953

  Fly  1375 Q--------CGLSLLQGNTIPKSLSRNI--------LRERI---YSNALDYFCGPPTCPNQSREQ 1420
            .        |...|.:..|:|     ||        |||:.   |.|..:...|.....|.:
Yeast  2954 ARKHNMPDVCISQLARIYTLP-----NIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTN--- 3010

  Fly  1421 LLEDIMILLKFWQTMRSE---KKHLVTSEVGDYDLTNASVSSTQMLAVRNNPETASLISGGGLVN 1482
                   |:.|....::|   .|.:..|::..|:..|.:.::    ||:.:...|...:..|..|
Yeast  3011 -------LVYFGTVQKAEFFTLKGMFLSKLRAYEEANQAFAT----AVQIDLNLAKAWAQWGFFN 3064

  Fly  1483 DYTRS-----MSASGNAVGMGMGVAG-------------------GGSSSG-------------- 1509
            |...|     :|.:.||:...:..||                   ...:||
Yeast  3065 DRRLSEEPNNISFASNAISCYLQAAGLYKNSKIRELLCRILWLISIDDASGMLTNAFDSFRGEIP 3129

  Fly  1510 ---WYNTIPHSTSTLS-KRSNRSKR------------LQYQ----------------------KD 1536
               |...||...::|| |.:|..:.            |.:|                      .|
Yeast  3130 VWYWITFIPQLLTSLSHKEANMVRHILIRIAKSYPQALHFQLRTTKEDFAVIQRQTMAVMGDKPD 3194

  Fly  1537 SYDK-------DYMKKRNLILE----LLAVELEFLITWYNPNSLPDLIVPGEEQITEWRNRPYKS 1590
            :.|:       :|:::.|.||:    |||:.||.|:...|..                    :||
Yeast  3195 TNDRNGRRQPWEYLQELNNILKTAYPLLALSLESLVAQINDR--------------------FKS 3239

  Fly  1591 TVWRDYARLAWCYNPALAVFLPQRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQE 1655
            |.                             ||::.||     |.|..|...|.|     |.|.
Yeast  3240 TT-----------------------------DEDLFRL-----INVLLIDGTLNY-----NRLP- 3264

  Fly  1656 SPDLVYILSWSPVTPIQALAYFSRQYPSHPLTAQYAVKTLSSYPAESVLPYIPQLVQALRHDTMG 1720
                                 |.|:.|..|...:   |.|..:....:.|||.....|
Yeast  3265 ---------------------FPRKNPKLPENTE---KNLVKFSTTLLAPYIRPKFNA------- 3298

  Fly  1721 YVVEFIKNISRRSQIVAHQLIWNMQTNMYMDEDQQHKDPNLYEALDQLSQSIIASFSGAAKRFYE 1785
               :||.|.......:.....|..:....:  |:..|..||......||            .|:.
Yeast  3299 ---DFIDNKPDYETYIKRLRYWRRRLENKL--DRASKKENLEVLCPHLS------------NFHH 3346

  Fly  1786 REFD----------------FFGKITAVSGEIRSFAKGIERKNACLAALSRIKVQGGCYLPSNPE 1834
            ::|:                .|.||......: .|.:|..      ::..|:.::|
Yeast  3347 QKFEDIEIPGQYLLNKDNNVHFIKIARFLPTV-DFVRGTH------SSYRRLMIRG--------- 3395

  Fly  1835 AMVLDIDYSSGTPMQSAAKAPYLAR-------FRVYRCGITEL----ETRAMEVSNN-----PNS 1883
                    ..|:....|.:.|.:..       |::||.....|    |||...:..|     |.|
Yeast  3396 --------HDGSVHSFAVQYPAVRHSRREERMFQLYRLFNKSLSKNVETRRRSIQFNLPIAIPLS 3452

  Fly  1884 ------QEDAKMTLGVESWQAAIFKVG---DDVRQDMLALQV------------ITIFK-NIFQQ 1926
                  .:....|...|.......|.|   ||: ||.:|.::            :||.| .||..
Yeast  3453 PQVRIMNDSVSFTTLHEIHNEFCKKKGFDPDDI-QDFMADKLNAAHDDALPAPDMTILKVEIFNS 3516

  Fly  1927 VGLDLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSGLSEYFQHQYGDESSKEFQAARANF 1991
            :..   :|                ||:...:|..     :.|...|:         :|...|..|
Yeast  3517 IQT---MF----------------VPSNVLKDHF-----TSLFTQFE---------DFWLFRKQF 3548

  Fly  1992 VKSMAAYSLIGYLLQIKDRHNGNIMIDK-DGHIIHIDF---GFMFESSPGGNIGFEPDMKLTDEM 2052
            ....:::..:.|::.|.:|....|.:|| .|::..::.   .|.:|.       .:|.:|..|
Yeast  3549 ASQYSSFVFMSYMMMINNRTPHKIHVDKTSGNVFTLEMLPSRFPYER-------VKPLLKNHD-- 3604

  Fly  2053 VMIMGGKMDSPAF 2065
               :....|||.|
Yeast  3605 ---LSLPPDSPIF 3614

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIIalpha	NP_001259191.1	PI4Ka	1600..1775	CDD:238443	31/174 (18%)
		PI4Kc_III_alpha	1836..2153	CDD:270711	52/272 (19%)
TRA1	NP_011967.1	TEL1	1232..3744	CDD:227365	400/2223 (18%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900