DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pi4KIIIalpha and Pik3cb

DIOPT Version :9

Sequence 1:	NP_001259191.1	Gene:	Pi4KIIIalpha / 31247	FlyBaseID:	FBgn0267350	Length:	2154	Species:	Drosophila melanogaster
Sequence 2:	NP_083370.2	Gene:	Pik3cb / 74769	MGIID:	1922019	Length:	1064	Species:	Mus musculus

Alignment Length:	858	Identity:	184/858 - (21%)
Similarity:	320/858 - (37%)	Gaps:	219/858 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly  1395 LRERIYSNALDYFCGPPTCPNQSREQLLEDIMILLKFWQTMRSE--------KKHLVTSEVGDYD 1451
            :|..:.:..|.:|.....|   ..:::.|..||.::......|.        ||..|.|.:.|
Mouse   263 IRNCVMNRTLPHFILVECC---KIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRVISHIWD-- 322

  Fly  1452 LTNASVSSTQMLAVRNNPETASLISGGGLVNDYTRSMSASGNAVGMGMGVAGGGSSSGWYNTIPH 1516
                          .|||...:|:.|..|..:.|           :.:.|..|         :.|
Mouse   323 --------------NNNPFQITLVKGNKLNTEET-----------VKVHVRAG---------LFH 353

  Fly  1517 STSTLSKR------SNRSKRLQYQKDSYD---KDYMKKRNLILELLAV----------------- 1555
            .|..|.|.      |.::..:..::..:|   .|..:...|...:.||
Mouse   354 GTELLCKTVVSSEISGKNDHIWNEQLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTINPSK 418

  Fly  1556 --------ELEFLITWYNP-------------------NSLPDLIVPGEEQITEWRNRPYKSTVW 1593
                    ::.:.:.|.|.                   :|.||.:   ||.:     .|..:...
Mouse   419 YQTIRKAGKVHYPVAWVNTMVFDFKGQLRSGDVILHSWSSFPDEL---EEML-----NPMGTVQT 475

  Fly  1594 RDYARLAW-------------CYNPALAVFLPQRIKNA--------------------EIIDEEV 1625
            ..||..|.             ||.|.....:.:..:.|                    ||:|.:.
Mouse   476 NPYAENATALHITFPENKKQPCYYPPFDKIIEKAAELASGDSANVSSRGGKKFLAVLKEILDRDP 540

  Fly  1626 SRLVCSDPIAV-------C--HIPEAL-KYLCTTK-NLLQESPDLVYILS-WSPVTPIQALAYFS 1678
            ...:|.:.:.:       |  :.|::| |.|.:.| |.|::...|..:|. |..:.|.:||....
Mouse   541 LSQLCENEMDLIWTLRQDCRENFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLD 605

  Fly  1679 RQYPSHPLTAQYAVKTLSSYPAESVLPYIPQLVQALRHDTM--GYVVEFIKNISRRSQIVAHQLI 1741
            ..||.. ...:|||..|.....|.:..|:.||||.|:::..  ..:..|:...:..::.:...|.
Mouse   606 FNYPDQ-YVREYAVGCLRQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALDNRRIGQFLF 669

  Fly  1742 WNMQTNMYMDEDQQHKDPNLYEALDQLSQSIIASFSGAAKRFYEREFDFFGKITAVSGEIR---- 1802
            |::::.::        .|.:......:.::......|..| ...::.:...|:..::..|:
Mouse   670 WHLRSEVH--------TPAVSVQFGVILEAYCRGSVGHMK-VLSKQVEALNKLKTLNSLIKLNAV 725

  Fly  1803 --SFAKGIERKNACLAALSRIKVQGGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYRC 1865
              |.|||.|..:.||...:..:.......|.||..::.::.......|.|..|..:|    ||
Mouse   726 KLSRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWL----VY-- 784

  Fly  1866 GITELETRAMEVSNNPNSQEDAKMTLGVESWQAAIFKVGDDVRQDMLALQVITIFKNIFQQVGLD 1930
                            :|:...:.::||      |||.|||:|||||.||::.:...::::.|||
Mouse   785 ----------------SSRAFGEDSVGV------IFKNGDDLRQDMLTLQMLRLMDLLWKEAGLD 827

  Fly  1931 LFLFPYRVVATAPGCGVIECVPNAKS-RD-QLGRQT---------DSGLSEYFQHQYGDESSKEF 1984
            |.:.||..:||....|:||.|..::: .| ||....         |:.|:...::..||:..:..
Mouse   828 LRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAATAAFNKDALLNWLKEYNSGDDLDRAI 892

  Fly  1985 QAARANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKDGHIIHIDFGFM---FESSPGGNIGFEPDM 2046
            :    .|..|.|.|.:..|:|.|.|||:.|||:.|.|.:.|||||.:   |:|..|......|.:
Mouse   893 E----EFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFI 953

  Fly  2047 KLTDEMVMIMGGKM-DSPAFKWFCELCVQAFLAVRPYQDAIVSLVSLMLDTGLPCFRG-QTINLL 2109
            ...|.:.:|..||. ::..|..|.:.|..|:|.:|.:.:..::|.:|||..|||.... :.|..|
Mouse   954 LTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYL 1018

  Fly  2110 KQRFVATKNNKEA 2122
            |......|:.:||
Mouse  1019 KDSLALGKSEEEA 1031

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIIalpha	NP_001259191.1	PI4Ka	1600..1775	CDD:238443	40/221 (18%)
Pi4KIIIalpha	NP_001259191.1	PI4Kc_III_alpha	1836..2153	CDD:270711	88/303 (29%)
Pik3cb	NP_083370.2	PI3K_p85B	35..112	CDD:197539
		PI3K_rbd	174..282	CDD:366310	4/21 (19%)
		C2_PI3K_class_I_beta_delta	318..495	CDD:176075	32/220 (15%)
		Nuclear localization signal (NLS). /evidence=ECO:0000250	404..412		0/7 (0%)
		PI3Ka_I	526..696	CDD:238444	35/178 (20%)
		PI3Kc_IA_beta	700..1061	CDD:270717	101/365 (28%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810