DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pi4KIIIalpha and PRKDC

DIOPT Version :9

Sequence 1:	NP_001259191.1	Gene:	Pi4KIIIalpha / 31247	FlyBaseID:	FBgn0267350	Length:	2154	Species:	Drosophila melanogaster
Sequence 2:	NP_008835.5	Gene:	PRKDC / 5591	HGNCID:	9413	Length:	4128	Species:	Homo sapiens

Alignment Length:	2357	Identity:	427/2357 - (18%)
Similarity:	711/2357 - (30%)	Gaps:	870/2357 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   195 FPPEL-LPIQK---------------GGGRDGTGSSSSAS------------------GTCGGSF 225
            ||.|: :|:::               .|..||....||.|                  |....|:
Human  1990 FPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSYSY 2054

  Fly   226 SSSERLAATHHFRPIIPRSMSGSLAQAQNASYDD---------GRQRC----------------- 264
            ||.:...||..||         ...|.....:||         .|..|
Human  2055 SSQDPRPATGRFR---------RREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGP 2110

  Fly   265 -AGGKPSKPS--------LHSYFSVPYDP-------------RTHFFTRYGSSFNQFPNMRVCES 307
             .|.:.|.|.        ||.....|..|             ....|..|...:.. |.:::..|
Human  2111 PQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLS-PLLQLAAS 2174

  Fly   308 PTKGGPRPLYRVPPFPIQHLQTIFA--------------VSKKLLTKDTLEHLDEQASDIFSLHQ 358
            ...||....|.|    ::.:.||.:              |....|....::|:......:|. |
Human  2175 ENNGGEGIHYMV----VEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRAVFR-H- 2233

  Fly   359 IKGYCYKSFSETLNLVLVTLLRELLQHQVDLP-----------TPFTKDVQEFVKRLFL------ 406
                         ||.::..|.|..:..:.:|           .|.:||....::.|.:
Human  2234 -------------NLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDL 2285

  Fly   407 ---NGQTELQNKQQDQERERREENGIAVVN-----KYKVNVMANAACVDLLVWAIRDETVDVDYN 463
               :.|..:|:.:..|          |:||     :||....|.|..:.|:
Human  2286 PPYDPQCGIQSSEYFQ----------ALVNNMSFVRYKEVYAAAAEVLGLI-------------- 2326

  Fly   464 VPSLQNGLQFLLKKE---ADKLCGRLSQKLNLELSHKIVMDHMPLLMVCLEGLGKLAHKFPNIAG 525
                   |:::::::   .:.||..::::|.   .|:..|:..  .:||   |.|:...||.:|.
Human  2327 -------LRYVMERKNILEESLCELVAKQLK---QHQNTMEDK--FIVC---LNKVTKSFPPLAD 2376

  Fly   526 TSISYLRDFLVAPSPILGKLHD--------------HAMQSLAQQKKEKE--------------- 561
            .       |:.|...:|.|.|.              ..|..|..|.|.|:
Human  2377 R-------FMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKV 2434

  Fly   562 --------------------LTPFKIAVQHSDSRTAVVIYG------DNQKPPGSGTGRSGHAAF 600
                                |.|....|.|..:.....:|.      ||.:.|.|.|.......|
Human  2435 CLDIIYKMMPKLKPVELRELLNPVVEFVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIF 2499

  Fly   601 ESLRDAAIENLSIALRAAHTLDQFCVPALVANVSNRLFTAEKSGSESQGECHKSNLVSLNII--- 662
            :..:|..|:.|         :|:  .|.|...:.|  |.:.::...|.   ....|::||.:
Human  2500 KLAKDVLIQGL---------IDE--NPGLQLIIRN--FWSHETRLPSN---TLDRLLALNSLYSP 2548

  Fly   663 -VMLGHVAVALKDTSKTTQNILQFFIQRFCKVPSEQNALIVDQLGCMIISQCETHVFDEIMKMFS 726
             :.:..:::|           ..|.::.....|...|.:....|     |:||...:........
Human  2549 KIEVHFLSLA-----------TNFLLEMTSMSPDYPNPMFEHPL-----SECEFQEYTIDSDWRF 2597

  Fly   727 RVTVQSASLAYTSDPEHRKQFHHVSDAVVNALGNIAANIQGDAEMLELLGKLLELFVQIGLDGER 791
            |.||.:.....|...:...| ....:..::|...:|..|:...:..:.      ...|.. ||..
Human  2598 RSTVLTPMFVETQASQGTLQ-TRTQEGSLSARWPVAGQIRATQQQHDF------TLTQTA-DGRS 2654

  Fly   792 SYDNTPG-------------------AQKASSRAGNLGMLIPVIAVLVRRLPPIKNPRQRLHKLF 837
            |:|...|                   |.|.|.|.               :..|:|:
Human  2655 SFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERL---------------QRAPLKS--------- 2695

  Fly   838 KDFWAYCVVMGFTNARL-WPADWYQG-VQQIAAKSPLLISQTAHKSDMRELNYTLAIKSDSVNEL 900
                   |...|...|| .|.|.... |:..|.::.||..:.....|..:|:...|.|..:..:.
Human  2696 -------VGPDFGKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKR 2753

  Fly   901 RSQILVLLEHSSDNVATAINKLSFAQCTYLLSVYWLEMLRVENADEPSLEPIMSYLCDTALQRDK 965
            ..:|...|:...|           ||.....|        ..:.|.|.::...|.|. |.||
Human  2754 EKEIKSELKMKQD-----------AQVVLYRS--------YRHGDLPDIQIKHSSLI-TPLQ--- 2795

  Fly   966 TGIWQCVKCVADQVFEK-FRNVLYAHDEIREKVLESQATLLLVYFNHIHKPIQMVADQYLSFLVD 1029
             .:.|....:|.|:|.. |..:|...|:.  |.|..:        |:|.:.:....:::|:....
Human  2796 -AVAQRDPIIAKQLFSSLFSGILKEMDKF--KTLSEK--------NNITQKLLQDFNRFLNTTFS 2849

  Fly  1030 RFPHLLWNRRVLWCMLDILQLLAYSLSLDPNE-ETPTLRVVSTPYTLQLMD---------SLPAR 1084
            .||..      :.|:.||....|..|||||.. ....|..:..|..::|::         .|||:
Human  2850 FFPPF------VSCIQDISCQHAALLSLDPAAVSAGCLASLQQPVGIRLLEEALLRLLPAELPAK 2908

  Fly  1085 ELRLKDFADRCQGIVNEAMKWA--PRSTRSHLQEY-------PNQIPTPVLAHHSGLALAFDSVV 1140
            .:|.|      ..:..:.::|.  .:..|| :.||       .::|.|..:...:.||.|.....
Human  2909 RVRGK------ARLPPDVLRWVELAKLYRS-IGEYDVLRGIFTSEIGTKQITQSALLAEARSDYS 2966

  Fly  1141 SSSAQHTGTMSKRPSCVNSDTPRFVSVLCLRSKYAGEISGLLSVLSEKDKAGLADRLVSDVWEAC 1205
            .::.|:...::|:.......|                         |.:|         |.||..
Human  2967 EAAKQYDEALNKQDWVDGEPT-------------------------EAEK---------DFWELA 2997

  Fly  1206 AEKSDARHRGALWRATAYLIICSEIS-------------------------------RKLLHAVA 1239
            :  .|..:..|.|::..|   ||..|                               :.||...|
Human  2998 S--LDCYNHLAEWKSLEY---CSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEA 3057

  Fly  1240 SSQLELFTESAMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFAWETEVTHPLAAY 1304
            ...|..|.:.||...::        :..|||.:.||
Human  3058 DQSLLTFIDKAMHGELQ--------KAILELHYSQE----------------------------- 3085

  Fly  1305 EGCKLVSKPILIAPHLIWLQLLSEMVDTAKYCNRDKVEMFC-------LLLHRC-LPVLKS---- 1357
                           |..|.||.:.||.|||..::.::.|.       :|||:. |..|:|
Human  3086 ---------------LSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQAL 3135

  Fly  1358 ----------SKQNRQVSTVGCRFKLL------------------------QC-GLSLLQG--NT 1385
                      |||....|.|..: :||                        :| .||.::.  ..
Human  3136 TEIQEFISFISKQGNLSSQVPLK-RLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSKIEEKLTP 3199

  Fly  1386 IPKSLSRNILRERIYSNALDYFCGPPTCPNQSREQLLEDIMILL---KFWQTMR---SEKKHLVT 1444
            :|:..|.|:.::           |.|:...:.:|| .|||..|:   ||...|:   |.:|.
Human  3200 LPEDNSMNVDQD-----------GDPSDRMEVQEQ-EEDISSLIRSCKFSMKMKMIDSARKQ--- 3249

  Fly  1445 SEVGDYDLTNASVSSTQMLAVRNNPETAS--LISGGGLVNDYTRSMSASGNAVGMGMGVAGGGSS 1507
                    .|.|::...:..:....:|..  |:|   .|..|.|.......:.|....|.
Human  3250 --------NNFSLAMKLLKELHKESKTRDDWLVS---WVQSYCRLSHCRSRSQGCSEQVL----- 3298

  Fly  1508 SGWYNTIPHSTSTLSKR---SNRSKRLQYQKDSYDKDYMKKRNLIL----ELLAVELEFLITWYN 1565
                 |:..:.|.|.:.   |..||.:...:|         :|::|    .::|..|
Human  3299 -----TVLKTVSLLDENNVSSYLSKNILAFRD---------QNILLGTTYRIIANAL-------- 3341

  Fly  1566 PNSLPDLIVPGEE-----------QITEWRNRPYKSTVWRDYARL--------------AWCYNP 1605
             :|.|..:...||           ..:|...:.......|.:..|              :|...|
Human  3342 -SSEPACLAEIEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGP 3405

  Fly  1606 A---------LAVFLPQRIK----NAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESP 1657
            |         ||.|..|:::    ||.:||.  :.|.....:.|..:.:|||.......|  :.|
Human  3406 AAGVIDAYMTLADFCDQQLRKEEENASVIDS--AELQAYPALVVEKMLKALKLNSNEARL--KFP 3466

  Fly  1658 DLVYILSWSPVTPIQALAYFSRQYPSHPLTAQYAVKTLSSYPAESVLPYIPQLVQALRHDTMGYV 1722
            .|:.|:               .:||...|:..  .|.:||.|....:.:|..:|..|..|....|
Human  3467 RLLQII---------------ERYPEETLSLM--TKEISSVPCWQFISWISHMVALLDKDQAVAV 3514

  Fly  1723 VEFIKNIS-RRSQIVAHQLIWNMQTNMYMDEDQQHKD-----------------PNLYEALDQLS 1769
            ...::.|: ...|.:.:..|.:.::..:.|....||:                 .:...||||||
Human  3515 QHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLS 3579

  Fly  1770 --QSIIASFSGAAK--------------RFYEREFDFFGKITAVS-GE-----IRSFAKGIER-- 1810
              :.:...:|...:              :.|||.:...|...|.. |.     |::|.|..::
Human  3580 NPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHF 3644

  Fly  1811 -KNACLAALSRIKVQGGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYRCGITELETRA 1874
             |..  :.|.|:|:..   .......::|.::..|..|......:|:::.|:|      |.....
Human  3645 GKGG--SKLLRMKLSD---FNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKV------EFLRNE 3698

  Fly  1875 MEVSNNPNSQE-------------DAKMTLGV--------------ESWQAAIFKVGDDVRQDML 1912
            :|:....:.:.             |.::|:..              |.....:.|.|:|:|||..
Human  3699 LEIPGQYDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQR 3763

  Fly  1913 ALQVITIFKNIFQQVGL----DLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSG------ 1967
            ..|:..:...|..|...    .|.|..|.||......|:||.:.|..:...|...|.|.
Human  3764 VEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAY 3828

  Fly  1968 LS-------EY--------FQHQYG-------------------------------------DES 1980
            ||       ||        .:|..|                                     ..|
Human  3829 LSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTS 3893

  Fly  1981 SKEFQAARANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKD-GHIIHIDFGFMFESSPGGNIGFEP 2044
            .:.|.|.|::|..|.|...:..::|.|.|||..|.|:..: |.:|.||||..|    |....|.|
Human  3894 PEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAF----GSATQFLP 3954

  Fly  2045 -----DMKLTDEMVMIM 2056
                 ..:||.:.:.:|
Human  3955 VPELMPFRLTRQFINLM 3971

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIIalpha	NP_001259191.1	PI4Ka	1600..1775	CDD:238443	44/207 (21%)
Pi4KIIIalpha	NP_001259191.1	PI4Kc_III_alpha	1836..2153	CDD:270711	65/316 (21%)
PRKDC	NP_008835.5	HEAT 1	288..323
		HEAT 2	1004..1040
		Interaction with C1D. /evidence=ECO:0000269\|PubMed:9679063	1503..1538
		Leucine-zipper	1503..1538
		TPR 1	1723..1756
		NUC194	1816..2202	CDD:400469	40/225 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2050..2073		8/31 (26%)
		KIP-binding	2436..3212		175/996 (18%)
		FAT	3023..3470	CDD:396714	101/557 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3200..3222		5/32 (16%)
		PIKKc_DNA-PK	3719..4015	CDD:270716	57/257 (22%)
		FATC	4097..4128	CDD:396715
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810