DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pi4KIIIalpha and PIK3C2A

DIOPT Version :9

Sequence 1:	NP_001259191.1	Gene:	Pi4KIIIalpha / 31247	FlyBaseID:	FBgn0267350	Length:	2154	Species:	Drosophila melanogaster
Sequence 2:	NP_001308307.1	Gene:	PIK3C2A / 5286	HGNCID:	8971	Length:	1686	Species:	Homo sapiens

Alignment Length:	1524	Identity:	295/1524 - (19%)
Similarity:	526/1524 - (34%)	Gaps:	489/1524 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   783 VQIGLDGER---SYDNTPGAQKASSRAGNLGMLIPVIAVLVRRL--------PPIKNPRQRLHKL 836
            :.:.|.|:.   ||..||..  .....|:|.:..||::..:.:|        ..:||.:.|....
Human   185 IYLSLPGQSPYFSYPLTPAT--PFHPQGSLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLE 247

  Fly   837 FKDFWAYCVVMGFTNARLWPA-------DWY---------------------QGVQQIAAKSP-- 871
            ..|       ...:|.::.|.       ||.                     :.|..:.||.|
Human   248 ITD-------SKVSNLQVSPKSEDISKFDWLDLDPLSKPKVDNVEVLDHEEEKNVSSLLAKDPWD 305

  Fly   872 -LLISQ--TAH-----KSDMRELNYTLAIKSDSVNELRSQILVLLEHSSDNVATAINKLSFAQCT 928
             :|:.:  ||:     |.:.:.|:.....:|.|:|...:|:.....|.|.......:.|......
Human   306 AVLLEERSTANCHLERKVNGKSLSVATVTRSQSLNIRTTQLAKAQGHISQKDPNGTSSLPTGSSL 370

  Fly   929 YLLSVYWLEMLRVENADEPS-------LEPIMSY----------LCDTALQRDKTGIWQCVKCVA 976
                   |:.:.|:|.:..:       |:....|          |.....||:..|....||...
Human   371 -------LQEVEVQNEEMAAFCRSITKLKTKFPYTNHRTNPGYLLSPVTAQRNICGENASVKVSI 428

  Fly   977 DQVFEKF-------------------RNVLYAHDEI--------------REKVLESQATLLLVY 1008
            |  .|.|                   :.:.:.||::              :|:||::
Human   429 D--IEGFQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQN-------- 483

  Fly  1009 FNHI---HKPIQMVADQYLSFLVDRFPHLLWNRRVLWCMLDILQLLAYSLSLD------PNEETP 1064
             ||.   |:.||....              |:..:.      ||||.:|....      .::|||
Human   484 -NHCLGSHEHIQNCRK--------------WDTEIR------LQLLTFSAMCQNLARTAEDDETP 527

  Fly  1065 T-----LRVVSTPYTLQLMDSLPARELRLKDFADRCQGIVNEAMKWAPRSTRSHLQEYPNQIPTP 1124
            .     |..:..| ..:.|...|..||                           |..|.||:...
Human   528 VDLNKHLYQIEKP-CKEAMTRHPVEEL---------------------------LDSYHNQVELA 564

  Fly  1125 VLAHHSGLAL------------AFDSVVSSSAQHTGTMSKRPSCVNSDTPRFVSVLCLRSKYAGE 1177
            :...:...|:            |.|.|.:.:.  |.::.|....||....:...|..|   :.||
Human   565 LQIENQHRAVDQVIKAVRKICSALDGVETLAI--TESVKKLKRAVNLPRSKTADVTSL---FGGE 624

  Fly  1178 ISGLLSVLSEKDKAGLADRLVSDVWEACAEKSDARHRGALWRATAYLIICSEISRKLLHAVASSQ 1242
                                         :.|.:..||:|......     ::|...|.|.....
Human   625 -----------------------------DTSRSSTRGSLNPENPV-----QVSINQLTAAIYDL 655

  Fly  1243 LELFTESAMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFAWETEVTHPLAAYEGC 1307
            |.|...|. .:..:|.|...:.::            ||.||.:.:..:||     .|.:::.
Human   656 LRLHANSG-RSPTDCAQSSKSVKE------------AWTTTEQLQFTIFA-----AHGISSN--- 699

  Fly  1308 KLVSKPILIAPHLIWLQLLSEMVDTAKYCNRDKVEMFCLLLHRCLPVLKSSKQNRQVSTVGCRFK 1372
                          |:.            |.:|..:.|.|.|....:.|.. |:::|.|....|.
Human   700 --------------WVS------------NYEKYYLICSLSHNGKDLFKPI-QSKKVGTYKNFFY 737

  Fly  1373 LLQCGLSLLQGNTIPKSLSRNILRERIYSNALDYFCGPPTCPNQSREQLLEDIMILLKFWQTMRS 1437
            |::.                         :.|..|      |.|..:..||.::
Human   738 LIKW-------------------------DELIIF------PIQISQLPLESVL----------- 760

  Fly  1438 EKKHLVTSEVGDYDLTNASVSSTQMLAVRNNPETASLISGGGLVNDYTRSMSASGNAVGMGMGVA 1502
               ||....:    |..:|.||......|..||....:|..  :.|:.|.::.....:.:.
Human   761 ---HLTLFGI----LNQSSGSSPDSNKQRKGPEALGKVSLP--LFDFKRFLTCGTKLLYLW---- 812

  Fly  1503 GGGSSSGWYNTIPHSTSTLSKRSNRSKRLQYQKD-------------SYDKDYMKKRNLILELLA 1554
                :|...|::|   .|::|:....:|:..|.|             ..|:..:::.|  ||.|.
Human   813 ----TSSHTNSVP---GTVTKKGYVMERIVLQVDFPSPAFDIIYTTPQVDRSIIQQHN--LETLE 868

  Fly  1555 VEL--EFLITWYNPNSLPDLIVPGEEQITEWRNRPYKSTVWRDYARLAWCYN-----PALAVFLP 1612
            .::  :.|...:..:||.   :..|::...|..|.|             |:.     |.:....|
Human   869 NDIKGKLLDILHKDSSLG---LSKEDKAFLWEKRYY-------------CFKHPNCLPKILASAP 917

  Fly  1613 QRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESPDLVYILSWSPVTPIQALAYF 1677
                |.:.::                       |..|.:||.:         |..:.|:.||...
Human   918 ----NWKWVN-----------------------LAKTYSLLHQ---------WPALYPLIALELL 946

  Fly  1678 SRQYPSHPLTAQYAVKTLSSYPAESVLPYIPQLVQALRHDTM--GYVVEFIKNISRRSQIVAHQL 1740
            ..::....:.: .||..:.:...:.:...:||.||||:::..  ..:|:|:.:.:..:..:||.|
Human   947 DSKFADQEVRS-LAVTWIEAISDDELTDLLPQFVQALKYEIYLNSSLVQFLLSRALGNIQIAHNL 1010

  Fly  1741 IWNMQTNMYMDEDQQHKDPNLYEALDQLSQSIIASFSGAAKRFYE---REFDFFGKITAVSGEIR 1802
            .|.::..::   |.|..  ..||.:      :.|..|...||..|   ::......:..|:.::|
Human  1011 YWLLKDALH---DVQFS--TRYEHV------LGALLSVGGKRLREELLKQTKLVQLLGGVAEKVR 1064

  Fly  1803 SFAKGIERKNACLAALSRIK---VQGGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYR 1864
            . |.|..|:.....::.|::   .:..|.||..|..:..:::..|.:...|.|
Human  1065 Q-ASGSARQVVLQRSMERVQSFFQKNKCRLPLKPSLVAKELNIKSCSFFSSNA------------ 1116

  Fly  1865 CGITELETRAMEVSNNPNSQEDAKMTLGVESWQAAIFKVGDDVRQDMLALQVITIFKNIFQQVGL 1929
                 :..:...|:.:|..:|...|           ||||:|:||||||||:|.|...|:.:.||
Human  1117 -----VPLKVTMVNADPMGEEINVM-----------FKVGEDLRQDMLALQMIKIMDKIWLKEGL 1165

  Fly  1930 DLFLFPYRVVATAPGCGVIECVPNAKSRDQL-------GRQTDSGLSEYFQHQYGDESSKEFQAA 1987
            ||.:..::.::|....|::|.||.:.:..::       |...|..|:|:.: :| :.|.:|::.|
Human  1166 DLRMVIFKCLSTGRDRGMVELVPASDTLRKIQVEYGVTGSFKDKPLAEWLR-KY-NPSEEEYEKA 1228

  Fly  1988 RANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKDGHIIHIDFG-FMFESSPGGNIGFEPD---MKL 2048
            ..||:.|.|...:..|:|.|.||||.|||:...||:.||||| |:..:...|:  |:.|   ..|
Human  1229 SENFIYSCAGCCVATYVLGICDRHNDNIMLRSTGHMFHIDFGKFLGHAQMFGS--FKRDRAPFVL 1291

  Fly  2049 TDEMV-MIMGGKMDSPAFKWFCELCVQAFLAVRPYQDAIVSLVSLMLDTGLPCFRG-QTINLLKQ 2111
            |.:|. :|.||:..:..|:.|.:||.||:..:|...:..::|:|||:.:|||.... |.:..::.
Human  1292 TSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLFLNLLSLMIPSGLPELTSIQDLKYVRD 1356

  Fly  2112 RFVATKNNKEAAAHMLAVIRNSYQNFRTR 2140
            .......:.||......:|.:|..:..|:
Human  1357 ALQPQTTDAEATIFFTRLIESSLGSIATK 1385

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIIalpha	NP_001259191.1	PI4Ka	1600..1775	CDD:238443	30/181 (17%)
Pi4KIIIalpha	NP_001259191.1	PI4Kc_III_alpha	1836..2153	CDD:270711	91/318 (29%)
PIK3C2A	NP_001308307.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..61
		Interaction with clathrin, sufficient to induce clathrin assembly. /evidence=ECO:0000269\|PubMed:16215232	2..142
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	348..367		3/18 (17%)
		PI3K_rbd	409..512	CDD:279173	23/133 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	618..639		7/52 (13%)
		C2A_PI3K_class_II	677..849	CDD:175979	45/280 (16%)
		PI3Ka_II	870..1038	CDD:238441	39/231 (17%)
		PI3Kc_C2_alpha	1043..1395	CDD:270720	102/376 (27%)
		PX_PI3K_C2_alpha	1426..1534	CDD:132822
		Interaction with PtdIns(4,5)P2-containing membranes. /evidence=ECO:0000250\|UniProtKB:Q61194	1488..1493
		C2B_PI3K_class_II	1560..1681	CDD:176027
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:11606566	1608..1619
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.810