DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pi4KIIIalpha and Smg1

DIOPT Version :9

Sequence 1:	NP_001259191.1	Gene:	Pi4KIIIalpha / 31247	FlyBaseID:	FBgn0267350	Length:	2154	Species:	Drosophila melanogaster
Sequence 2:	NP_001026984.1	Gene:	Smg1 / 233789	MGIID:	1919742	Length:	3658	Species:	Mus musculus

Alignment Length:	2398	Identity:	431/2398 - (17%)
Similarity:	771/2398 - (32%)	Gaps:	733/2398 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 MTASDKYTYQRTVLCLARVLAGIQPTPWDKVQTLFRYCPQENAAGVFCLDTRAQDAVIALGIYFL 67
            :||:::..:...||..|....|:.....|...|:  :|                |.||..|:..|
Mouse   438 VTAANQVFFSEAVLTAANECVGVLLGSLDPSMTI--HC----------------DMVITYGLDQL 484

  Fly    68 EGGCQHEGQIVPYLLRLAKCLPKAVWIDDAR--SNKVERVRIPSAE------------------- 111
            | .||..|  ..|::.:...|...|...:.:  |:.||::.|||::
Mouse   485 E-NCQTCG--TDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAV 546

  Fly   112 ----------------------KFSFCLNTLLSDIAA--KCPDSREEIILNQVET---------- 142
                                  :.:..||.||..:..  .|.:.:.|...|.|..
Mouse   547 YQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPDACSEIKHEAFQNHVFNIDNANFVVIF 611

  Fly   143 -LSALANIVKSSRDSSSAPPPIILCKATVPLLFGLARSMGRYASNDPPLLCRIFP----PELLPI 202
             ||||..|       .:|...:|...|..|.:|.|.........:|   |...||    ..|..:
Mouse   612 DLSALTTI-------GNAKNSLIGMWALSPTVFALLSKNLMIVHSD---LAVHFPAIQYAVLYTL 666

  Fly   203 QKGGGRD----GTGSSSSASGTCGGSFSSSERLAATHHFRPIIPRSMSGSLAQAQNASYDDGRQR 263
            .....|.    .:..|||:.....|:..|:...|...||..|:  ::.|.|.:..|.:.|
Mouse   667 YSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIIL--NLLGILLKKDNLNQD----- 724

  Fly   264 CAGGKPSKPSLHSYFSVPYDPRTHFFTRYGSSFNQFPNMRVCESPTKGGPRPLYRVPPFPIQHLQ 328
                  ::..|.::                 :......|:..|:..     ||:.:|.|
Mouse   725 ------TRKLLMTW-----------------ALEVAVLMKKSETYA-----PLFSLPSF------ 755

  Fly   329 TIFAVSKKLLTKDTLEHLDEQASDIFSLHQIKGYCYKSFSETLNLVLVTLLRELLQHQVD----- 388
              ...||.||....:|.::.......|||.:..               :|..:|||..||
Mouse   756 --HKFSKGLLANTLVEDVNICLQACSSLHALSS---------------SLPDDLLQRCVDVCRVQ 803

  Fly   389 LPTPFTKDVQEFVKR---------LFLNGQTELQNKQQDQERERREENGIAV---VNKYKVNVMA 441
            |....|:..|.|.|.         |..|..||:|            |..:|:   ::|...|...
Mouse   804 LVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQ------------EISLALRSHMSKAPSNTFH 856

  Fly   442 NAACVDLLVWAI--------RDETVD-VDYNVPSLQNGLQFLLKKEADKLCGRLSQKLNLELSHK 497
            .....|::.:.:        :|..:: :.|:...|....|..:.:...|....|.|....|.:..
Mouse   857 PQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQF 921

  Fly   498 IVMDHM--PLLMVCLEGLGKLAHKFPNIAGTSISYLRDFLVAPSPILGKLHDHAMQSLAQQKKEK 560
            .|:..:  |        ||:....|..|.|               |:..|..|.:      ..::
Mouse   922 TVLSKLRTP--------LGRAQDTFQTIEG---------------IIRSLAAHTL------NPDQ 957

  Fly   561 ELTPFKIAVQ---HSDSRTAVVI---YGDNQKPPGSGTGRSGHAAFESLRDAAIENLSIALRAAH 619
            :::.:..|..   |..::..:|:   |.:|.:       :..:.|:|...:|......:.....:
Mouse   958 DVSQWTTADNDEGHGSNQLRLVLLLQYLENLE-------KLMYNAYEGCANALTSPPKVIRTFFY 1015

  Fly   620 TLDQFC-------------------VPALVANVSNRLFTAEKSGSESQGECHKSNLVSLNIIVML 665
            |..|.|                   .||:.......|.|..|:.|.:||.       .|.:.:|:
Mouse  1016 TNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTNSLTQGS-------ELEVTIMM 1073

  Fly   666 GHVA------------VALKDTSKTTQNIL------QFFIQRFCK--VPSEQNALIVDQLGCMII 710
            ...|            :|:..:|...:|:|      |....||.|  |..:::...:..:.|.|.
Mouse  1074 VVEALCELHCPEAIQGIAVWSSSAVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCIS 1138

  Fly   711 SQCETHVFDEIMKMFSRVTVQSASLAYTSDPEHRKQFHHVSDAVVNALGNIAANIQGDAEMLELL 775
            |      ||:.:...:.....|||..::.:.|.||..             ::.:|....|::..|
Mouse  1139 S------FDKSVLTLANAGRNSASPKHSLNGESRKTV-------------LSKSIDSSPEVISYL 1184

  Fly   776 G-KLLELFVQIG-LDGERSYDNTPGAQKASSRAGNLGMLIPVIAVLVRRLPPIKNPRQRLHKLFK 838
            | |..|.::.|. ....:.:.|.....|.:|.:.:|                         .|..
Mouse  1185 GNKACECYISIADWAAVQEWQNAVHDLKKNSSSTSL-------------------------NLKA 1224

  Fly   839 DFWAYCVVMGFTNARLWPADWYQGVQQI----AAKSPLLISQTAHKSDMRELNYTLAIKSDSVNE 899
            ||.....:..|.:     .::.:..:|:    .....||...:..|.||::|...:.  |....|
Mouse  1225 DFNYIKSLSSFES-----GEFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNML--SPDPRE 1282

  Fly   900 LRSQILVLLEHSSDNVATAINKLSFAQCTYLLS---VYWLEM--------LRVENADEPSLEPIM 953
            |:..|.|.|..||..:|||:|.:...|....|:   |.:|:.        ||:.........|::
Mouse  1283 LQKSIEVQLLRSSVFLATALNHMEQDQKWQSLTENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVL 1347

  Fly   954 SYL---CDTALQRDKTGIWQCVKCVADQVFEKFRNVLYAHDEIREKVLESQATLLLVYFNHIHKP 1015
            |.|   |.:||:...:.......|:.....:..|: ...|| :|..:   ||....:|.|.:.:.
Mouse  1348 STLQLYCSSALENTVSNRLSTEDCLIPLFSDALRS-CKQHD-VRPWM---QALRYTMYQNQLLEK 1407

  Fly  1016 IQMVADQYLSFLVDRFPHLLWNRRVLWCMLDILQLLAYSLSLDPNEETPTLRVVSTPYTLQLMDS 1080
            |:.......|.|::                  |.|.|...:......:...|:::....:||..:
Mouse  1408 IKEQTVPIRSHLME------------------LGLTAAKFARKRGNVSLATRLLAQCSEVQLGKT 1454

  Fly  1081 LPARELRLKDFAD-RCQGIVNEAMKWAPRSTRSHLQEYPNQIPTPVLAHHSGLALAFDSVVSSSA 1144
            ..|::| ::.|.. ..||.|:|  ||.|.                       |.:....::.::.
Mouse  1455 TTAQDL-VQHFKKLSTQGQVDE--KWGPE-----------------------LDIEKTKLLYTAG 1493

  Fly  1145 QHTGTMSKRPSCVNSDTPRFVSVLCLRSKYAGEISGLLSVLSEKDKAGLADRLVSDVWEACAEKS 1209
            |.|..|....||..|        .|..:|  .|.:...|:|:      || :.|...|:..:.:.
Mouse  1494 QSTHAMEMLSSCAIS--------FCKSAK--AEYAVAKSILT------LA-KWVQAEWKEISGQL 1541

  Fly  1210 DARHRGALWRATAYLIICSEISRKLL--------HAVASSQLELFTESAMETAVECWQWVLTARQ 1266
            ...:|....:..:.|   |.:||.:|        :.|......:.:||.:...|....::|....
Mouse  1542 RQVYRAQQQQNLSGL---STLSRNILALIELPSANTVGEEHPRIESESTVHIGVGEPDFILGQLY 1603

  Fly  1267 DLELCFIQEMVSAWQTTFEKRMGLFAWETEVTHPLAAYEGCKLVSKPILIAPHLIWLQLLSEMVD 1331
            .|......|:..:|...   ....:.|..:|....:..||.:|:.:......:|        :.|
Mouse  1604 HLSSVQAPEVAKSWAAL---ASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNL--------LPD 1657

  Fly  1332 TAKYCNRDKVEMFCLLLHR-CLPV----------LKSSKQNRQVSTVGCRFKLL--QC-GLSLLQ 1382
            |  ....:|..::.:|... |.|.          :..|:.|.....|...::.|  .| .||.|.
Mouse  1658 T--ITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEDDDMVDVIWRQLISSCPWLSELD 1720

  Fly  1383 GNTIPKSLS--RNILRERIYS----NALDYFC--------------GPPTCPNQSREQLLEDIMI 1427
            .|.....:.  |.:: :||:|    :...||.              .|....:...||..:|:::
Mouse  1721 ENATEGVIKVWRKVV-DRIFSLYKLSCSAYFTFLKLNAGQVLLDEDDPRLHLSHRAEQSTDDVIV 1784

  Fly  1428 LLKFWQTMRSEKKHLVTSEVGDYDLTNASVSST--------QMLAVRNNPE-------------- 1470
            :... :.:|...||  ..|:..|.......:.|        |:.:..|:||
Mouse  1785 MATL-RLLRLLVKH--AGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRV 1846

  Fly  1471 ---TASLISGGGLVNDYTRSMS----ASGN----AVGMGMG-VAG---------GGSSSGWYNTI 1514
               :..||....:|.  |.|:|    ||||    |:...:| :.|         |||
Mouse  1847 AQDSPHLILYPAIVG--TISLSSESQASGNKYSSAIPTLLGNIQGEELLVSECEGGS-------- 1901

  Fly  1515 PHSTSTLSK---RSNRSKRLQYQKDSYDK--DYMKKRN----LILELLAVELEFLITWYNPNSLP 1570
            |.::...:|   :|..::.....:|.|.|  |.:...|    |.:::|..||..:...::...|.
Mouse  1902 PPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLG 1966

  Fly  1571 DL------IVPGEEQITEWRNRPYKSTVWRDYARLAWCYNPALAVFLPQRIKNAEIIDEEVSRLV 1629
            .|      ::...:|:.:...|...:...|...::|.......|:..|      .:...|..|.:
Mouse  1967 VLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKP------IVFALEHVRSI 2025

  Fly  1630 CSDPIAVCH-----------IPEALKYLCTTKNLLQESPDLVYILSWSPVTPIQALAYFSRQYPS 1683
            .:.|....|           |..||:.|.|..|..:...      ||.|...|.
Mouse  2026 TAAPAETPHEKWFQDNYGDAIDNALEKLKTPSNPAKPGS------SWIPFKEIM----------- 2073

  Fly  1684 HPLTAQYAVKTLSSY--PAESVLPYIPQLVQALRHDTMGYVVEFIKNISRRSQIVAHQL-----I 1741
              |:.|...:..:||  ..:.:.|::..:...        .:.....:|.|..:..|.:     |
Mouse  2074 --LSLQQRAQKRASYILRLDEISPWLAAMTNT--------EIALPGEVSARDTVTIHSVGGTITI 2128

  Fly  1742 WNMQTN----MYMDEDQQHKDPNLYEALDQLS--------QSIIASFSGA-----AKRFYEREFD 1789
            ...:|.    :::..|.: ..|.|::.|:.|.        .||:.:....     ..||:.|.:.
Mouse  2129 LPTKTKPKKLLFLGSDGK-SYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYS 2192

  Fly  1790 F------FGKITAVSGEIRSFA--KGIERKNACLAALSRIKVQGGCYLPSNPEAM--VLDIDYSS 1844
            .      .|.|..|.|....|.  |..:::.|.|.|.   |.|.....|.||..:  ..::.||
Mouse  2193 VTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQ---KAQDSYQTPQNPSIVPRPSELYYS- 2253

  Fly  1845 GTPMQSAAKAPYLARFRVYRCGITELETRAMEVSNNPNSQEDAKMTLGVESWQAAIFKVGDDVRQ 1909
                                                                     |:|..::.
Mouse  2254 ---------------------------------------------------------KIGPALKT 2261

  Fly  1910 DMLALQV------ITIFKNIFQQVGLDLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSGL 1968
            ..|:|.|      :.:.|.:.:::          :.||.|.....|...:..:.|:..|.|.|
Mouse  2262 VGLSLDVSRRDWPLHVMKAVLEEL----------MEATPPNLLAKELWSSCTTPDEWWRVTQS-- 2314

  Fly  1969 SEYFQHQYGDESSKEFQAARANFVKSMAAYSLIGYLLQIKDRHNGNIMID-KDGHIIHIDFGFMF 2032
                                  :.:|.|..|::||::.:.|||..|::|| ..|.::|||:...|
Mouse  2315 ----------------------YARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCF 2357

  Fly  2033 ESSPGGNIGFEPDMKLTDEMVMIMGGKMDSPAFKWFCELCVQAFLAVRPYQDAIVSLV 2090
            |......:..:...::|..:...:|.......|:..||   |....:|..::.:::|:
Mouse  2358 EKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCE---QVLHIMRRGRETLLTLL 2412

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIIalpha	NP_001259191.1	PI4Ka	1600..1775	CDD:238443	34/204 (17%)
Pi4KIIIalpha	NP_001259191.1	PI4Kc_III_alpha	1836..2153	CDD:270711	41/264 (16%)
Smg1	NP_001026984.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..99
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	116..142
		SMG1	629..1240	CDD:292413	134/775 (17%)
		HEAT	1815..1850		4/34 (12%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1896..1917		6/28 (21%)
		PIKKc_SMG1	2119..2424	CDD:270714	68/393 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3565..3588
		FATC	3628..3657	CDD:280430
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5032
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900