DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp3A and kif4

DIOPT Version :9

Sequence 1:	NP_001284830.1	Gene:	Klp3A / 31240	FlyBaseID:	FBgn0011606	Length:	1212	Species:	Drosophila melanogaster
Sequence 2:	NP_957117.1	Gene:	kif4 / 393796	ZFINID:	ZDB-GENE-040426-1545	Length:	1248	Species:	Danio rerio

Alignment Length:	1286	Identity:	434/1286 - (33%)
Similarity:	680/1286 - (52%)	Gaps:	164/1286 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSSEDPSC--VAVALRVRPLVQSELDRGCRIAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKD 63
            |:.||...  |.||||.||||..|::.||:..: ......|||.|..::::|::||||....|::
Zfish     1 MTKEDEKAIPVRVALRCRPLVPKEINEGCQCCL-NFVPSEPQVIVGNDKAFTFDYVFDPTTEQEE 64

  Fly    64 LFETCVQAKVKKLLNGYNVTILAYGQTGSGKTYTMGTAFNGVLDDH--VGVIPRAVHDIFTAIAE 126
            :|.|.|...:..|..||:.|:|||||||||||::||..:....::.  ||||||.:..||...|:
Zfish    65 VFNTAVSPLLSGLFKGYHATVLAYGQTGSGKTFSMGGTYTSEQENEPTVGVIPRVIRRIFQEKAK 129

  Fly   127 MQSEFRFAVTCSFVELYQEQFYDLFSSKTRDKATVDIRE-VKNRIIMPGLTELVVTSAQQVTDHL 190
             :::..|.:..|::|:|.|:..||..: ::||..:.||| .|:.|.:.||||..|.:|.::...|
Zfish   130 -RTDCEFVLAVSYLEIYNEEILDLLCT-SKDKPVISIREDPKDGIKIVGLTERDVLNAHEMVCCL 192

  Fly   191 IRGSAGRAVAATAMNETSSRSHAIFTLTLVATKLDGKQSVTTSRFNLVDLAGSERCSKTLASGDR 255
            ..|::.|.|.:||||..|||||||||::|...:...|..|..|:.:|||||||||..||.|.|||
Zfish   193 EMGNSARTVGSTAMNAASSRSHAIFTISLEQRRNGDKTDVMVSKLHLVDLAGSERQKKTKAEGDR 257

  Fly   256 FKEGVNINKGLLALGNVINALGSGQAAG-YIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNV 319
            .|||::||:|||:|||||:|||.....| ::|||.|||||||||||||||.|||||||||||.|:
Zfish   258 LKEGISINRGLLSLGNVISALGDESKKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNI 322

  Fly   320 AETLSTLRYADRALQIKNKPVVNLDPHAAEVNMLKDVIQKLRVELLSGGKMSSSLISAVGAAGLG 384
            .||::||||||||.:||||||:|:||.|||:..||..:|:|:|.||.             |.| |
Zfish   323 EETINTLRYADRARKIKNKPVLNVDPRAAEMKRLKQQVQELQVMLLH-------------ARG-G 373

  Fly   385 AIPCEESLAGSMANAAEIQRLKEQVRTLQDRNRKLQQELHQSLLDLTEKEMRAHIAEQAHDKLRS 449
            ..|.   |:|| ..|..:.::.||.|:|||:|.||.:||.:|:........|..:.||.::||:|
Zfish   374 VAPV---LSGS-EPAENVSKVLEQNRSLQDQNSKLSRELSESVGQTAAMFERMLMMEQTNEKLQS 434

  Fly   450 HVSELKN----KLDQREQAQFGNENTNGDNEMRD-FSLLVN--RVHVELQR-------------- 493
            .:.||:.    |:|.:...:     |..|.|:|: ..::.|  ::.:||||
Zfish   435 KLDELRQHAACKVDLQRLVE-----TLEDQELRENVEVIQNLQQIILELQRESAGIAATIDAITS 494

  Fly   494 -TQEELESQGHESRQRLSSRSHTEGGESGG-DEVHEMLHSHSEEYTNKQMNFAGELRNINRQLDL 556
             ....||:..|.|.....:::....|:.|. .|..:...:::.::..:|...:.||..:|:.|.|
Zfish   495 GNASSLEAAAHCSPSTAGAQAEICPGQDGAVPEAKDSPEAYTAQHALRQAQMSKELIELNKVLAL 559

  Fly   557 KQELHERIMRNFSRLD--SDDEDVKLRLCNQKIDDLEAERRDLMDQLRNIKSKDISAKLAEERRK 619
            |:...:::.:|.|.|:  .......::.....:..|:.|:.||:..|::.|.....|||:|:|||
Zfish   560 KEAFVKKMCQNDSHLEPMQTQYQESIQSLQAAVGSLQKEKEDLVQTLQSAKKDTNQAKLSEQRRK 624

  Fly   620 RLQLLEQEISDLRRKLITQANLLKIRDKEREKIQNLSTEIRTMKESKVKLIRAMRGESEKFRQWK 684
            |||.||.::::|::||..|:.|||:::.....:..|:.||:.||..:|:|:|.|:.:|||||.||
Zfish   625 RLQELEGQMTELKKKLQDQSKLLKLKESSVRNVAKLNQEIQAMKSQRVQLMRQMKDDSEKFRLWK 689

  Fly   685 MVREKELTQLKSKDRKMQSEIVRQQTLHSKQRQVLKRKCEEALAANKRLKDALERQASAQAQRHK 749
            ..::||:.|||.||||.|.|:::.:....||..||:||.|||.||||||||||::: |..|::.|
Zfish   690 QKKDKEVLQLKEKDRKRQYEMLKLERDFQKQASVLRRKTEEAAAANKRLKDALQKR-SEVAEKRK 753

  Fly   750 YKDNGGSAAGSSNANAKTDSWVDRELEIILSLIDAEHSLEQLMEDRAVINNHYHLLQQEKTSDPA 814
            ...|    .|:.:...:..:|:..|:|:::|..:|...|:.|:|||.::......|:|:     .
Zfish   754 DVQN----RGAESVTNRVKNWLLNEIEVLVSTEEARRHLQDLLEDRKMLAEEISQLRQQ-----M 809

  Fly   815 EAAEQ-------------------------ARILASLEEELEMRNAQISDLQQKVCPTDLDSRIR 854
            ||.|:                         ::.:.||:.|:.:|:|||:||||||...|.:.|::
Zfish   810 EAGEKPVSKVRRRTLTISELEGKCELEASISKQVESLDTEMALRSAQIADLQQKVLDADNEGRMK 874

  Fly   855 SLAEGVQSLGESRTVSKQLLKTLVQQRRLQASSLNEQRTTLDELRAQLLDAQQQEDAASKRLRLL 919
            ...:.:.::.|:::..|.|:..:|..:.|.|    :|.:.|.:.:|.|||.|:......|.:..:
Zfish   875 QRWDSITTIIEAKSALKILMAEVVASKTLNA----KQESELQQEKANLLDMQKVLCDERKLMSAM 935

  Fly   920 QSQHEEQMLAQQRAYEEKVSVLIRTANQRWAEARSPAEDQQRNQIL----EELLSSREALQQELD 980
            ..:|:..::..::.::|||..|:.....:      |...:::.:::    :|||...:..::|::
Zfish   936 DMEHQSHLVEMEQRHQEKVLYLLGQLQNK------PVALEEQQELVSKREQELLQRLQFQEEEIE 994

  Fly   981 KLRAKNKSKSKAVKSEPQDLDDSFQIVD-GNETVVLSDVSDDPD----WVPSTSKSK-------R 1033
            |||..:....| :|.|.:.......:.. .:...|||.|::.||    ::|...|:|       |
Zfish   995 KLRELSDQNQK-LKEENEQYKQKLHLAQLTSAKKVLSAVNESPDTSFEYIPPKPKAKMHSTARAR 1058

  Fly  1034 IQS--DSRNVISP-----------PEKQDANVTSLGNSSIQSLNSTSATEDGKRCKGCKCRTKCT 1085
            .|.  |...::||           |||  |..|..|:...: ||            ||.||.:|.
Zfish  1059 QQEPIDLDELMSPSDSEKEEDEWRPEK--AEKTRRGSKKAK-LN------------GCACRGRCV 1108

  Fly  1086 TKRCGCLSGNNACSETCVC-KSNCRNPLNLKDHASQCGDGDGQKDETEDADKSDDDGDDEP-QTS 1148
            .|.|.|..|...|.|.|:| ...||   |:::.:|.      ..:||.|..|......::| ...
Zfish  1109 NKLCRCRKGKMTCGENCLCDHEKCR---NMENRSSV------DLNETTDVFKDSSVLPEDPTAVG 1164

  Fly  1149 KENAVKFVTPEAP------GKVVASPKQTLQEPKAAATPLMNSNVVEDING 1193
            ..:|..|..|..|      |:|...|.....|.|.........:..||..|
Zfish  1165 SRDATFFTPPCVPPTKKEIGEVWQLPADLKLEKKPVPAENERESDSEDSEG 1215

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp3A	NP_001284830.1	KISc_KIF4	7..336	CDD:276823	164/334 (49%)
		Kinesin	14..335	CDD:278646	160/324 (49%)
		Smc	<396..>994	CDD:224117	183/651 (28%)
		FlaC	522..633	CDD:225888	30/113 (27%)
		TCR	1075..1110	CDD:281618	14/35 (40%)
kif4	NP_957117.1	KISc_KIF4	9..339	CDD:276823	164/332 (49%)
		KISc	10..345	CDD:214526	170/337 (50%)
		BST2	568..644	CDD:293321	24/75 (32%)
		DUF3435	<938..1082	CDD:288746	30/150 (20%)
		TCR	1100..1136	CDD:281618	16/38 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	319	1.000	Domainoid score	I1222
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H69022
Inparanoid	1	1.050	612	1.000	Inparanoid score	I881
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D369179at2759
OrthoFinder	1	1.000	-	-		FOG0003140
OrthoInspector	1	1.000	-	-		oto39568
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	LDO	PTHR47969
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3568
SonicParanoid	1	1.000	-	-		X2108
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.900