Protein Alignment Klp3A and KIF5A

DIOPT Version :10

• Sequence 1: NP_525053.1
• Gene: Klp3A / 31240
• FlyBaseID: FBgn0011606
• Length: 1212
• Species: Drosophila melanogaster
• Sequence 2: NP_004975.2
• Gene: KIF5A / 3798
• HGNCID: 6323
• Length: 1032
• Species: Homo sapiens
• Alignment Length: 1205
• Identity: 316/1205 - (26%)
• Similarity: 508/1205 - (42%)
• Gaps: 253/1205 - (20%)

  Fly     1 MSSEDPSC-VAVALRVRPLVQSELDRGCR-IAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKD 63
            |:..:..| :.|..|.|||.|:|:.||.: |.:.:..|.    .|...:.|.::.||..:.:|:.
Human     1 MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDS----VVIGGKPYVFDRVFPPNTTQEQ 61

  Fly    64 LFETCVQAKVKKLLNGYNVTILAYGQTGSGKTYTMGTAFNGVLDDH--VGVIPRAVHDIFTAIAE 126
            ::..|....||.:|.|||.||.|||||.||||:||    .|.|.|.  :|:|||...|||..|..
Human    62 VYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTM----EGKLHDPQLMGIIPRIARDIFNHIYS 122

  Fly   127 MQSEFRFAVTCSFVELYQEQFYDLFSSKTRDKATVDIREVKNRI-IMPGLTELVVTSAQQVTDHL 190
            |.....|.:..|:.|:|.::..||...   .|..:.:.|.|||: .:.|.||..|:|.:::.|.:
Human   123 MDENLEFHIKVSYFEIYLDKIRDLLDV---TKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVI 184

  Fly   191 IRGSAGRAVAATAMNETSSRSHAIFTLTLVATKLDGKQSVTTSRFNLVDLAGSERCSKTLASGDR 255
            ..|.:.|.||.|.|||.|||||:||.:.:....::.:|.: :.:..||||||||:.|||.|.|..
Human   185 DEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKL-SGKLYLVDLAGSEKVSKTGAEGAV 248

  Fly   256 FKEGVNINKGLLALGNVINALGSGQAAGYIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNVA 320
            ..|..||||.|.||||||:||..| ...|:|||.||:||:||||||||..|.|..|.||:.||.|
Human   249 LDEAKNINKSLSALGNVISALAEG-TKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDA 312

  Fly   321 ETLSTLRYADRALQIKNKPVVNLDPHAAE-----------VNMLKDVIQKLRVEL---LSGGKMS 371
            ||.|||.:..||..|||...|||:..|.:           ....|:.|.||..||   .:|..:.
Human   313 ETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVP 377

  Fly   372 SSLISAVGAAGLGAIPCEESLAGSMANAAEIQRLKEQVRTLQDRNRKLQQELHQSLLDLTEKEMR 436
            .:...|...|.|||..|||:.....::..        ||...:..:|.::|:.:....|.:|:..
Human   378 ETERLAGEEAALGAELCEETPVNDNSSIV--------VRIAPEERQKYEEEIRRLYKQLDDKDDE 434

  Fly   437 AHIAEQAHDKLRSHVSELKNKLDQREQAQFGNENTNGDNEMRDFSLLVNRVHVELQRTQEELESQ 501
            .:...|..:||:..:      |||.|..    .:|.||||             ::||....|:|:
Human   435 INQQSQLIEKLKQQM------LDQEELL----VSTRGDNE-------------KVQRELSHLQSE 476

  Fly   502 GHESRQRLSSRSHTEGGESGGDEVHEMLHSHSEEYTNKQMNFAGELRNINRQLDLKQELHERIMR 566
                            .::..|||.|:|.:..|...|....              .||:.|:..:
Human   477 ----------------NDAAKDEVKEVLQALEELAVNYDQK--------------SQEVEEKSQQ 511

  Fly   567 NFSRLDSDDEDVKLRLC----NQKIDDLEAERRDLMDQLRNIKSKDISAKLAEERRKRLQLLEQE 627
            |...:|...:.|...|.    .|::.::...:|..:.::.|...||:|..........:: |..|
Human   512 NQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIK-LPVE 575

  Fly   628 ISDLRRKLITQANLLKIRDKEREKIQNLSTEIRTMKESKVKLIRAMRGESEKFRQWKMVREKELT 692
            ||....:..|.|.|  ...|.:.:::::....|.::..:|:..|.|.....:....:::..:...
Human   576 ISGAIEEEFTVARL--YISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEA 638

  Fly   693 QLKSKDRKMQS-----------------EIVRQQTLHSKQRQVLKRKCEEALAANKRLKDALERQ 740
            :::|....|||                 |:.:.|...:.....||.|..:...|:: :|.|||.|
Human   639 KIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADE-VKKALELQ 702

  Fly   741 ASAQAQRH-----KYKDNGGSAAGSSNANAKTDSWVDRELEIILSLIDAEHSLEQLMEDRAVINN 800
            ..:..:.|     :.:|       ..|...||   :| ||:.:...:..|  ||:|..|...:.:
Human   703 MESHREAHHRQLARLRD-------EINEKQKT---ID-ELKDLNQKLQLE--LEKLQADYEKLKS 754

  Fly   801 HYHLLQQEKTSDPAEAA------EQAR-ILASLEEELEMRNAQISDLQQKVCPTDLDSRIRSLAE 858
            ..|    ||::...|..      ||:: .|..|||.: .|..|.....:|:...|:.:|::..||
Human   755 EEH----EKSTKLQELTFLYERHEQSKQDLKGLEETV-ARELQTLHNLRKLFVQDVTTRVKKSAE 814

  Fly   859 -------GVQSLGESRTVSKQLLKTLVQQRRLQASSLNEQ--RTTLDELRAQLLDAQQQEDAASK 914
                   |:.|       .||.: :.::....|.:.:::|  |...| ||.:|...:::..|.::
Human   815 MEPEDSGGIHS-------QKQKI-SFLENNLEQLTKVHKQLVRDNAD-LRCELPKLEKRLRATAE 870

  Fly   915 RLRLLQSQHEEQMLAQQRAYEEKVSVLIRTANQRWAEARSPAEDQQRNQILEELLSSREALQQEL 979
            |::.|:...:|   |::.|.::|         :|:                          |||:
Human   871 RVKALEGALKE---AKEGAMKDK---------RRY--------------------------QQEV 897

  Fly   980 DKLRAKNKSKSKAVKSEPQDLDDSFQIVDGNETVVLSDVSDDPDWVPSTSKSKRIQSDSRNVISP 1044
            |:::...:.||...:              |:...:...|.  |...|::|.:           :|
Human   898 DRIKEAVRYKSSGKR--------------GHSAQIAKPVR--PGHYPASSPT-----------NP 935

  Fly  1045 PEKQDANVTSLGNSSIQS-----LNST-SATEDGKRCKGCKCRTKCTTKRCGCLSGNNACSETCV 1103
            ...:.....|..||..|:     |.:| |:|.|      ......||:.  |..|.....:....
Human   936 YGTRSPECISYTNSLFQNYQNLYLQATPSSTSD------MYFANSCTSS--GATSSGGPLASYQK 992

  Fly  1104 CKSNCRNPLNLKDHASQ--CGDGDGQKDETEDADK 1136
            ...:..|..::.|:.|.  ||      .|.||..|
Human   993 ANMDNGNATDINDNRSDLPCG------YEAEDQAK 1021

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp3A	NP_525053.1	KISc_KIF4	7..336	CDD:276823	141/333 (42%)
		Smc	<453..>985	CDD:440809	113/573 (20%)
		Metallothio	<1071..1115	CDD:395080	6/43 (14%)
KIF5A	NP_004975.2	KISc_KHC_KIF5	7..327	CDD:276820	141/332 (42%)
		Microtubule-binding	174..315		72/142 (51%)
		Necessary for interaction with ZFYVE27. /evidence=ECO:0000250|UniProtKB:P33175	271..361		40/90 (44%)
		Interaction with BICD2. /evidence=ECO:0000269|PubMed:20386726	353..1032		167/840 (20%)
		PRK03918	<409..920	CDD:235175	123/646 (19%)
		Khc_CBD_cc	832..901	CDD:467880	19/107 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	906..939		8/59 (14%)
		Globular	907..1032		30/156 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	978..1010		5/33 (15%)