DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Klp3A and KIF4B

DIOPT Version :9

Sequence 1:	NP_001284830.1	Gene:	Klp3A / 31240	FlyBaseID:	FBgn0011606	Length:	1212	Species:	Drosophila melanogaster
Sequence 2:	NP_001092763.1	Gene:	KIF4B / 285643	HGNCID:	6322	Length:	1234	Species:	Homo sapiens

Alignment Length:	1298	Identity:	434/1298 - (33%)
Similarity:	667/1298 - (51%)	Gaps:	207/1298 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 VAVALRVRPLVQSELDRGCRIAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKDLFETCVQAKV 73
            |.||||.||||..|:..||::.:. ...|..||.|..::|:||::|||....|:::|...|...:
Human    10 VRVALRCRPLVPKEISEGCQMCLS-FVPGETQVVVGTDKSFTYDFVFDPCTEQEEVFNKAVAPLI 73

  Fly    74 KKLLNGYNVTILAYGQTGSGKTYTMGTAFNGVLDDH--VGVIPRAVHDIFTAIAEMQSEFRFAVT 136
            |.:..|||.|:||||||||||||:||.|:....::.  ||:|||.:..:|..| :.:|:|.|.:.
Human    74 KGIFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGIIPRVIQLLFKEI-DKKSDFEFTLK 137

  Fly   137 CSFVELYQEQFYDLFSSKTRDKATVDIRE-VKNRIIMPGLTELVVTSAQQVTDHLIRGSAGRAVA 200
            .|::|:|.|:..||. ..:|:||.::||| .|..|.:.||||..|..|......|.:|:..|.||
Human   138 VSYLEIYNEEILDLL-CPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVA 201

  Fly   201 ATAMNETSSRSHAIFTLTLVATKLDGKQSVTTSRFNLVDLAGSERCSKTLASGDRFKEGVNINKG 265
            :||||..|||||||||:::...|...|.....|:.:|||||||||..||.|.|||.|||:|||:|
Human   202 STAMNSQSSRSHAIFTISIEQRKKSDKNCSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRG 266

  Fly   266 LLALGNVINALGSGQAAGYIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNVAETLSTLRYAD 330
            ||.|||||:|||..:...::|||.|||||||||||||||.|||||||||||.|:.||||||||||
Human   267 LLCLGNVISALGDDKKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLSTLRYAD 331

  Fly   331 RALQIKNKPVVNLDPHAAEVNMLKDVIQKLRVELLS--GGKMSSSLISAVGAAGLGAIPCEESLA 393
            ||.:|||||:||:|||.||:|.||..:|:|:|.||.  ||.:..|         :.|.|.|
Human   332 RARKIKNKPIVNIDPHTAELNHLKQQVQQLQVLLLQAHGGTLPGS---------INAEPSE---- 383

  Fly   394 GSMANAAEIQRLKEQVRTLQDRNRKLQQELHQSLLDLTEKEMRAHIAEQAHDKLRSHVSELKN-- 456
                   .:|.|.|:.::|.:.|.||.:.|.::.....:...|..:.||.::||.:.:.||:.
Human   384 -------NLQSLMEKNQSLVEENEKLSRCLSKAAGQTAQMLERIILTEQVNEKLNAKLEELRQHV 441

  Fly   457 --KLDQREQAQFGNENTNGDNEMRDFSLLVNRVHVELQRTQEELESQ-GHESRQRLSSRSHTEGG 518
              |||.::..:     |..|.|:::        :||:....::|.:| ..|:....::...|...
Human   442 ACKLDLQKLVE-----TLEDQELKE--------NVEIICNLQQLITQLSDETVACTAAAIDTAVE 493

  Fly   519 ESGGDEVHEMLHSHSEEYTNK----QMNFAGELRNINRQLDLKQELHERIMRNFSRLDSDDEDVK 579
            |....|........|:.:|.:    |...:.|:..:|..|.||:.|..::.:|.::|.......:
Human   494 EEAQVETSPETSRSSDAFTTQHALHQAQMSKEVVELNNALALKEALVRKMTQNDNQLQPIQFQYQ 558

  Fly   580 LRLCNQKID--DLEAERRDLMDQLRNIKSKDISAKLAEERRKRLQLLEQEISDLRRKLITQANLL 642
            ..:.|.:::  :|:.|:.:|:.:|:..|.....|||:|.|.|.||.||.:|:||::||..|:.||
Human   559 DNIKNLELEVINLQKEKEELVRELQTAKKNVNQAKLSEHRHKLLQELEGQIADLKKKLNEQSKLL 623

  Fly   643 KIRDKEREKIQNLSTEIRTMKESKVKLIRAMRGESEKFRQWKMVREKELTQLKSKDRKMQSEIVR 707
            |:::.....:..|:.||..||..:|:|:|.|:.::|||||||..::||:.|||.:|||.|.|:::
Human   624 KLKESTERTVSKLNQEIWMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLK 688

  Fly   708 QQTLHSKQRQVLKRKCEEALAANKRLKDALERQASAQAQRHKYKDNGGSAAGSSNANAKTDSWVD 772
            .:....||..||:||.|||.||||||||||::|.....:|.:.:.:     |.....|:..:|:.
Human   689 LERNFQKQSSVLRRKTEEAAAANKRLKDALQKQREVTDKRKETQSH-----GKEGIAARVRNWLG 748

  Fly   773 RELEIILSLIDAEHSLEQLMEDRAVINNHYHLLQQEKTS--DP-----------AEAAEQ----- 819
            .|:|:::|..:|:..|..|:|||.::......|:::|.|  :|           :|...|
Human   749 NEIEVMVSTEEAKRHLNDLLEDRKILAQDVVQLKEKKESRENPPPKLRKCTFSLSEVHGQVLESE 813

  Fly   820 ---ARILASLEEELEMRNAQISDLQQKVCPTDLDSRIRSLAEGVQSLGESRTVSKQLLKTLVQQ- 880
               .:.:.|||.|:|:|:|||:|||||:...:.:.|.:...|.:.::.|::...|.|:..||..
Human   814 DCITKQIESLETEMELRSAQIADLQQKLLDAESEDRPKQCWENIATILEAKCALKYLIGELVSSK 878

  Fly   881 ----------RRLQASSLNEQRTTLD----------ELRAQLLDAQQQEDAASKRLRLLQSQHEE 925
                      |:.:||..:.|:...:          ||:|:|:..:||..  .|.|.|:....|.
Human   879 IHVTKLENSLRQSKASCADMQKMLFEEQNHFSEIETELQAELVRMEQQHQ--EKVLYLVSQLQES 941

  Fly   926 QMLAQQ--RAYEEKVSVLIRTANQRWAEARSPAEDQQRNQILEELLSSREALQQELDKLRAKNKS 988
            ||..:|  ::..||...|:.|...:..|.....|..::||   :||...|.::|:|..|:..::.
Human   942 QMAEKQLEKSASEKEQQLVSTLQCQDEELEKMREVCEQNQ---QLLQENEIIKQKLILLQVASRQ 1003

  Fly   989 KSKAVKSEPQDLDDSFQIVDGNETVVLSDVSDDPDWVPSTSKSKRIQSDSRNVISPPEK---QDA 1050
            |...                 |:|::..|.|.  :::|...|..|::          ||   |..
Human  1004 KHLP-----------------NDTLLSPDSSF--EYIPPKPKPSRVK----------EKFLEQSM 1039

  Fly  1051 NVTSLGNSSIQSLNSTSATEDG------------------------KRCKGCKCRTKCTTKRCGC 1091
            ::..|...|..|:|.   .|||                        |..:||.|:..|..|:|||
Human  1040 DIEDLKYCSEHSVNE---HEDGDGDGDSDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGNKQCGC 1101

  Fly  1092 LSGNNACSETCVC-KSNCRNPLNLKDHASQCGDGDGQKDETEDAD---KSDDDGDDEPQTSKENA 1152
            ....:.|...|.| .:.|||       ..|..|..|..::|:|::   |.:|..:..|..|..|.
Human  1102 RKQKSDCGVDCSCDPTKCRN-------RQQGKDSLGTVEQTQDSEGSFKLEDPTEVTPGLSFFNP 1159

  Fly  1153 VKFVTPEAPGKVVASPKQTLQEPKAAATPLMNSNVVEDINGPK--LAKMSGLA---FDTPKRK 1210
            |                        .|||  ||.:::::...:  |:|.:..|   ||.|:.|
Human  1160 V------------------------CATP--NSKILKEMCDMEQVLSKKTAPAPSPFDLPESK 1196

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Klp3A	NP_001284830.1	KISc_KIF4	7..336	CDD:276823	170/329 (52%)
		Kinesin	14..335	CDD:278646	166/323 (51%)
		Smc	<396..>994	CDD:224117	183/652 (28%)
		FlaC	522..633	CDD:225888	31/116 (27%)
		TCR	1075..1110	CDD:281618	12/35 (34%)
KIF4B	NP_001092763.1	KISc_KIF4	8..337	CDD:276823	170/329 (52%)
		KISc	9..343	CDD:214526	175/335 (52%)
		SbcC	406..995	CDD:223496	173/611 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	494..513		3/18 (17%)
		Interaction with PRC1. /evidence=ECO:0000250\|UniProtKB:O95239	663..1234		160/609 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	712..737		10/29 (34%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:O95239	793..798		0/4 (0%)
		Globular. /evidence=ECO:0000250	1000..1234		56/262 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1007..1030		6/41 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1052..1076		4/26 (15%)
		Pre-SET	<1072..1122	CDD:282838	15/56 (27%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000250\|UniProtKB:O95239	1086..1144		19/64 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1143		5/20 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1183..1234		5/14 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	313	1.000	Domainoid score	I1299
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	605	1.000	Inparanoid score	I942
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D369179at2759
OrthoFinder	1	1.000	-	-		FOG0003140
OrthoInspector	1	1.000	-	-		otm41233
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	O	PTHR47969
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3568
SonicParanoid	1	1.000	-	-		X2108
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	12	11.900