Protein Alignment Klp3A and CENPE

DIOPT Version :10

• Sequence 1: NP_525053.1
• Gene: Klp3A / 31240
• FlyBaseID: FBgn0011606
• Length: 1212
• Species: Drosophila melanogaster
• Sequence 2: NP_001804.2
• Gene: CENPE / 1062
• HGNCID: 1856
• Length: 2701
• Species: Homo sapiens
• Alignment Length: 1524
• Identity: 350/1524 - (22%)
• Similarity: 596/1524 - (39%)
• Gaps: 398/1524 - (26%)

  Fly     5 DPSCVAVALRVRPLVQSELDRGCRIAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKDLFETCV 69
            :...|||.:|||||...|...|....|....|......|:.::|:.::.||..:::.|:::|...
Human     3 EEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGSKSFNFDRVFHGNETTKNVYEEIA 67

  Fly    70 QAKVKKLLNGYNVTILAYGQTGSGKTYT-MGTAFNGVLDDHVGVIPRAVHDIFTAIAEMQSEFRF 133
            ...:...:.|||.||.|||||.|||||| ||:      :||:||||||:||||..|.:.... .|
Human    68 APIIDSAIQGYNGTIFAYGQTASGKTYTMMGS------EDHLGVIPRAIHDIFQKIKKFPDR-EF 125

  Fly   134 AVTCSFVELYQEQFYDLFSSKTRDKATVDIREVKNRIIMPGLTELVVTSAQQVTDHLIRGSAGRA 198
            .:..|::|:|.|...||.....:.|..:...:|...:.:..|||.||.:::.....:.:|...|.
Human   126 LLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNRNVYVADLTEEVVYTSEMALKWITKGEKSRH 190

  Fly   199 VAATAMNETSSRSHAIFTLTLVA------TKLDGKQSVTTSRFNLVDLAGSERCSKTLASGDRFK 257
            ...|.||:.|||||.||.:.|.:      :..:|  ||..|..||||||||||.::|.|:|.|.|
Human   191 YGETKMNQRSSRSHTIFRMILESREKGEPSNCEG--SVKVSHLNLVDLAGSERAAQTGAAGVRLK 253

  Fly   258 EGVNINKGLLALGNVINALGSGQAAGYIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNVAET 322
            ||.|||:.|..||.||..|..||..|:|.||.|||||:||:|||||:.|.:|..::|..::  ||
Human   254 EGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPVSFD--ET 316

  Fly   323 LSTLRYADRALQIKNKPVVN------------------LDPHAAEVNM----------------- 352
            |:.|::|..|..:||.|.||                  |.....||::
Human   317 LTALQFASTAKYMKNTPYVNEVSTDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLE 381

  Fly   353 LKDVIQKLRVELLSG-------------------------------------------------- 367
            .||::||::.|.:..
Human   382 EKDLLQKVQNEKIENLTRMLVTSSSLTLQQELKAKRKRRVTWCLGKINKMKNSNYADQFNIPTNI 446

  Fly   368 ----GKMSSSLISAVGAAGLGAIPCEES--LAGSMANAAEIQ-----RLKEQVRTLQDRNRKLQQ 421
                .|:|.:|:..:..:     .|.||  .:.::...:||:     :|..| ..::.....|:.
Human   447 TTKTHKLSINLLREIDES-----VCSESDVFSNTLDTLSEIEWNPATKLLNQ-ENIESELNSLRA 505

  Fly   422 ELHQSLLDL----TEKEMRAHIAEQAHDKLRSHVSELKNKLDQREQ--------------AQFGN 468
            :....:||.    ||||......::.:|.......|.|.|.||..|              |:..|
Human   506 DYDNLVLDYEQLRTEKEEMELKLKEKNDLDEFEALERKTKKDQEMQLIHEISNLKNLVKHAEVYN 570

  Fly   469 ENTNGDNEMRDFSLLVNRVHVELQRTQEELESQGHES---------------RQRLSSRSHTEGG 518
            ::.  :||:.....|:.....::::.||.::||..|:               :|...:....|..
Human   571 QDL--ENELSSKVELLREKEDQIKKLQEYIDSQKLENIKMDLSYSLESIEDPKQMKQTLFDAETV 633

  Fly   519 ESGGDEVHEMLHSHSEEYTNKQMNFAGELRNINR---------------QLDLKQELHERIMRNF 568
            ..........|.|.:.|...|....|...:.:..               |:||::|| :......
Human   634 ALDAKRESAFLRSENLELKEKMKELATTYKQMENDIQLYQSQLEAKKKMQVDLEKEL-QSAFNEI 697

  Fly   569 SRLDS--DDEDVKLRLCN----QKIDDL-----------EAERRD--LMDQLRNIKS------KD 608
            ::|.|  |.:..|..|||    .||.||           ||.|.:  |:.:|:::.|      |:
Human   698 TKLTSLIDGKVPKDLLCNLELEGKITDLQKELNKEVEENEALREEVILLSELKSLPSEVERLRKE 762

  Fly   609 ISAKLAE----------------ERRKRLQLLEQEISDLRRKLITQANLLKIRDKE--------- 648
            |..|..|                .:..|:|.|.:||...:..|.|..:..|..|:|
Human   763 IQDKSEELHIITSEKDKLFSEVVHKESRVQGLLEEIGKTKDDLATTQSNYKSTDQEFQNFKTLHM 827

  Fly   649 ----------------REKIQNLSTEIRTMKES----KVKLIRAMRGESEKFRQWKMVREK--EL 691
                            .::|.|||.|.:....|    |.:|....:...||.|:   |:|:  |:
Human   828 DFEQKYKMVLEENERMNQEIVNLSKEAQKFDSSLGALKTELSYKTQELQEKTRE---VQERLNEM 889

  Fly   692 TQLK----SKDRKMQSEIVRQQTLHSKQRQVLKRKCEEALAANKRLKDALERQASAQAQRHKYKD 752
            .|||    ::|..:|: :.|::||.:::   |::..||.....:...|..:.|.|.|.:|.:.|.
Human   890 EQLKEQLENRDSTLQT-VEREKTLITEK---LQQTLEEVKTLTQEKDDLKQLQESLQIERDQLKS 950

  Fly   753 NGGSAAGSSNANAKTDSWVDRELEIILSLIDAEHSLEQLMEDRAVINNHYHLLQQEKTSDPAEAA 817
            :   ...:.|.|..|...:...||.:....:..::|:..:.:....|.|    .:|.|.:..:..
Human   951 D---IHDTVNMNIDTQEQLRNALESLKQHQETINTLKSKISEEVSRNLH----MEENTGETKDEF 1008

  Fly   818 EQARILASLEEELEMRNAQI------------------------SDLQQKV---------CPTDL 849
            :|..:....:::||.:|.|.                        ::|||.:         ..|||
Human  1009 QQKMVGIDKKQDLEAKNTQTLTADVKDNEIIEQQRKIFSLIQEKNELQQMLESVIAEKEQLKTDL 1073

  Fly   850 DSRIRSLAEGVQSLGESRTVSKQLLK--TLVQQRRLQA------------------SSLNEQRTT 894
            ...|....|..:.|   |.:..:|.|  .:|.|.:..|                  ..|.|:...
Human  1074 KENIEMTIENQEEL---RLLGDELKKQQEIVAQEKNHAIKKEGELSRTCDRLAEVEEKLKEKSQQ 1135

  Fly   895 LDELRAQLLDAQQQEDAASKRLRLLQSQHEE---QMLAQQRAYEEKVSVLIRTANQRWAEARSPA 956
            |.|.:.|||:.|::.....|::..:::...|   :.|..:....|::. |.:..|:.:.|.:|..
Human  1136 LQEKQQQLLNVQEEMSEMQKKINEIENLKNELKNKELTLEHMETERLE-LAQKLNENYEEVKSIT 1199

  Fly   957 EDQQRNQILEEL----------------------LSSREAL----------QQELDKLRAKNKSK 989
            :::   ::|:||                      |.::|.|          |:.:|:|| ::.|:
Human  1200 KER---KVLKELQKSFETERDHLRGYIREIEATGLQTKEELKIAHIHLKEHQETIDELR-RSVSE 1260

  Fly   990 SKAVKSEPQDLDDSFQIVDGNETVVLSDVSDDPDWVPSTSKSKRIQSDSRNVISPPEKQDANVTS 1054
            ..|.....|||:.|...:. .|..||.:..:....|...|:::...::...:......:|:  |:
Human  1261 KTAQIINTQDLEKSHTKLQ-EEIPVLHEEQELLPNVKEVSETQETMNELELLTEQSTTKDS--TT 1322

  Fly  1055 LGNSSIQSLNSTSATEDGKRCKGCKCRTKCTTKRCGCLSGNNACSETCVCKSNCRNPLNLKDH-- 1117
            |....::.|......::.:.      ..|..||....|   ....|....|.:     .||:|
Human  1323 LARIEMERLRLNEKFQESQE------EIKSLTKERDNL---KTIKEALEVKHD-----QLKEHIR 1373

  Fly  1118 --ASQCGDGDGQKDETEDADKSDDD-----GDDEPQTSKENAVKFVTPEAPG------------K 1163
              .::..:...:::::.:..:.|::     .:.|....|::|:..:..|..|            |
Human  1374 ETLAKIQESQSKQEQSLNMKEKDNETTKIVSEMEQFKPKDSALLRIEIEMLGLSKRLQESHDEMK 1438

  Fly  1164 VVASPK---QTLQEPKAAATPLMNSNVVE 1189
            .||..|   |.|||...:.:..:..|:.|
Human  1439 SVAKEKDDLQRLQEVLQSESDQLKENIKE 1467

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Klp3A	NP_525053.1	KISc_KIF4	7..336	CDD:276823	133/335 (40%)
		Smc	<453..>985	CDD:440809	146/739 (20%)
		Metallothio	<1071..1115	CDD:395080	6/43 (14%)
CENPE	NP_001804.2	KISc_CENP_E	6..329	CDD:276825	133/333 (40%)
		SMC_prok_B	345..1131	CDD:274008	147/811 (18%)
		SMC_prok_B	828..1715	CDD:274008	127/679 (19%)
		SMC_prok_B	1332..2129	CDD:274008	25/150 (17%)
		SMC_N	1730..>2573	CDD:481474
		Kinetochore-binding domain	2126..2476
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2355..2376
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2508..2533
		Globular autoinhibitory domain. /evidence=ECO:0000250	2510..2698
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2588..2701