Protein Alignment PsGEF and ARHGEF10L

DIOPT Version :10

• Sequence 1: NP_996333.4
• Gene: PsGEF / 31224
• FlyBaseID: FBgn0264598
• Length: 2777
• Species: Drosophila melanogaster
• Sequence 2: NP_060595.3
• Gene: ARHGEF10L / 55160
• HGNCID: 25540
• Length: 1279
• Species: Homo sapiens
• Alignment Length: 1521
• Identity: 264/1521 - (17%)
• Similarity: 444/1521 - (29%)
• Gaps: 575/1521 - (37%)

  Fly   451 AQACPPPTSRHSQP------------KANAGQDQKQETEAAKDQDKQQD---------------- 487
            |.:.|||     ||            .::..:|...|.....|.|.::|
Human     2 ASSNPPP-----QPAIGDQLVPGVPGPSSEAEDDPGEAFEFDDSDDEEDTSAALGVPSLAPERDT 61

  Fly   488 DSPAEMAAAVTVTPQKPVATGSGSG-----SNSAAM--------------------------ALN 521
            |.|.....::.||...|.|...|:|     ||..|.                          ||.
Human    62 DPPLIHLDSIPVTDPDPAAAPPGTGVPAWVSNGDAADAAFSGARHSSWKRKSSRRIDRFTFPALE 126

  Fly   522 DEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQ 586
            ::||   .|.:..|.|               ..||..|:.||...        |.:......||:
Human   127 EDVI---YDDVPCESP---------------DAHQPGAERNLLYE--------DAHRAGAPRQAE 165

  Fly   587 ATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPF--TMTKRLTRTE 649
            ..|..||..::...::.           :...|..       .|...:.|..|  .::..|||.:
Human   166 DLGWSSSEFESYSEDSG-----------EEAKPEV-------EVEPAKHRVSFQPKLSPDLTRLK 212

  Fly   650 ERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTL 714
            |             |..:.:..:.|.|.|                   ..|:..|.......:|
Human   213 E-------------RYARTKRDILALRVG-------------------GRDMQELKHKYDCKMT- 244

  Fly   715 EIFRRSGAGATTITSTDLGQNNVHISTRLG-----AAVGLGSEEHTLATTATGTTTVMSLQRTTS 774
            ::.:.:.:|    |...|.:..:.:..::.     ..:|...||.            |.|...:.
Human   245 QLMKAAKSG----TKDGLEKTRMAVMRKVSFLHRKDVLGDSEEED------------MGLLEVSV 293

  Fly   775 TRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFT 839
            :.|:|       ||                   |::....|.:.|....||..|..::..|.::.
Human   294 SDIKP-------PA-------------------PELGPMPEGLSPQQVVRRHILGSIVQSEGSYV 332

  Fly   840 AALHFGVDRFVQPLGERK-DLISPNDHRTLFQNIDELL------RIAEDILEQLCSSDQQDQEPQ 897
            .:|...:..:..||.|.: ..:|....:.:|..:.|:|      :||  :..::...|..::...
Human   333 ESLKRILQDYRNPLMEMEPKALSARKCQVVFFRVKEILHCHSMFQIA--LSSRVAEWDSTEKIGD 395

  Fly   898 MNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPD-LTMF- 960
            :..||   .|| :.:...|..|.|....| ..::.|:..|...|:.|:....|  ..|| :|::
Human   396 LFVAS---FSK-SMVLDVYSDYVNNFTSA-MSIIKKACLTKPAFLEFLKRRQV--CSPDRVTLYG 453

  Fly   961 -IHRPLQHFREILKLMQLLAGNC---HVD--------TE---------EHKNFSTVINELQAAYR 1004
             :.:|:|.|.:.:.|:|.:..|.   |.|        ||         |.|..:..:.|:|...:
Human   454 LMVKPIQRFPQFILLLQDMLKNTPRGHPDRLSLQLALTELETLAEKLNEQKRLADQVAEIQQLTK 518

  Fly  1005 EITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTL 1069
            .::..|.|.:.|..|:..|.|           |:..|..|.|.:           |:.:|
Human   519 SVSDRSSLNKLLTSGQRQLLL-----------CETLTETVYGDR-----------GQLIK----- 556

  Fly  1070 LFSDIIVFAKVSRDRVLFITEEPIPIANV------------VDSCFHMRKKTTEFRLTVDPNGRL 1122
                       |::|.:|:..:.:..||:            :.|...:..|......|..|..::
Human   557 -----------SKERRVFLLNDMLVCANINFKPANHRGQLEISSLVPLGPKYVVKWNTALPQVQV 610

  Fly  1123 AE--SPTGYCAPDLTRTPKRGARRKS--------LILRAPSL--ELKAVWQNL-LQRQIFLVNAA 1174
            .|  ...|....|.......||::.|        :.|..|.|  ||:.:.::| :..||.|:.:.
Human   611 VEVGQDGGTYDKDNVLIQHSGAKKASASGQAQNKVYLGPPRLFQELQDLQKDLAVVEQITLLIST 675

  Fly  1175 LGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSAT 1239
            |..|                 ..::.:|.|....:.|..|  :.:|:::           :|||
Human   676 LHGT-----------------YQNLNMTVAQDWCLALQRL--MRVKEEE-----------IHSA- 709

  Fly  1240 GVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRIL-------NKTGTPKS--------SALHL 1289
                                           .|||:.       :|:|.|.|        :.|..
Human   710 -------------------------------NKCRLRLLLPGKPDKSGRPISFMVVFITPNPLSK 743

  Fly  1290 ANWMKGQLDKQQQQARLAAIARSQENVSAEDEQLIFNSDSEQDERITYWTR------QQLEKRTK 1348
            .:|:        .:..||.|...:||     :......|.::..:..:|..      .....|..
Human   744 ISWV--------NRLHLAKIGLREEN-----QPGWLCPDEDKKSKAPFWCPILACCIPAFSSRAL 795

  Fly  1349 ELNLAKENGGLSARPNFGGKRLSGVEELSMSATSD-IYSTSEAEGVTSVISQSHSTTSDSQITVR 1412
            .|.|    |.|...|  ....|.|...:|.|.... ::.....||....: :..|....|..||:
Human   796 SLQL----GALVHSP--VNCPLLGFSAVSTSLPQGYLWVGGGQEGAGGQV-EIFSLNRPSPRTVK 853

  Fly  1413 SSPIVLDKLAVCRHC------HKNCQQSGPGGARPGGGGGVNNSSSTPVLLC-----NSLKVQHS 1466
            |.|:....|  |...      ....:...|..|.|        |::....:|     .|:.:..|
Human   854 SFPLAAPVL--CMEYIPELEEEAESRDESPTVADP--------SATVHPTICLGLQDGSILLYSS 908

  Fly  1467 QSSPNRC---CK----------------LSNGIAGATEMANPKNSNRT-----------GTGTGS 1501
            ..:..:|   |:                |..|:...|..|.|:.|...           ..|.|.
Human   909 VDTGTQCLVSCRSPGLQPVLCLRHSPFHLLAGLQDGTLAAYPRTSGGVLWDLESPPVCLTVGPGP 973

  Fly  1502 VTSMSSSTITGELSSKVVASSSRRETGDTESDVAQLITDEISQSQSEATDDSVGAMPNSSRSAGE 1566
            |.::.|      |...|.||...|.|      |.:..|.:..||.....|::|.........:|.
Human   974 VRTLLS------LEDAVWASCGPRVT------VLEATTLQPQQSFEAHQDEAVSVTHMVKAGSGV 1026

  Fly  1567 GNPVITGSRLR--------HLDPPVSVSS-----LP-------------NGHCSAGVGGGSPKPL 1605
            .....:|:.:|        ||. .:::::     ||             .|....|...|....|
Human  1027 WMAFSSGTSIRLFHTETLEHLQ-EINIATRTTFLLPGQKHLCVTSLLICQGLLWVGTDQGVIVLL 1090

  Fly  1606 PPPRRTRVVAQMCQEPPRPKANQQVKAIVTITETARIMRSSPTKGSGSGSATGTGSVGGSPAKYA 1670
            |.||                    ::.|..||            |.|..|..|
Human  1091 PVPR--------------------LEGIPKIT------------GKGMVSLNG------------ 1111

  Fly  1671 ACHCRCTPEDFTHAQLSPDKMEHQTCKLLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLC 1735
              ||                          .|               ::.:.:..:.|||.....
Human  1112 --HC--------------------------GP---------------VAFLAVATSILAPDILRS 1133

  Fly  1736 GQERI--PKEENSTPTIAVVPPTPDS 1759
            .||..  |:.|...|......|.|||
Human  1134 DQEEAEGPRAEEDKPDGQAHEPMPDS 1159

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PsGEF	NP_996333.4	DUF4799	<99..209	CDD:318308
		C2	286..>381	CDD:425499
		PDZ_canonical	645..716	CDD:483948	11/70 (16%)
		RhoGEF	835..1007	CDD:470622	42/201 (21%)
		PRK12323	<2304..>2415	CDD:481241
ARHGEF10L	NP_060595.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..91		19/93 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	159..197		7/55 (13%)
		RhoGEF	317..501	CDD:238091	41/192 (21%)
		PH_19	526..681	CDD:465965	37/209 (18%)
		WD40_2	789..1279	CDD:465964	87/488 (18%)
		WD40 repeat	927..970	CDD:293791	6/42 (14%)
		WD40 repeat	978..1007	CDD:293791	11/40 (28%)
		WD40 repeat	1016..1046	CDD:293791	3/29 (10%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1135..1163		9/25 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1186..1208