Protein Alignment PsGEF and Arhgef10

DIOPT Version :10

• Sequence 1: NP_996333.4
• Gene: PsGEF / 31224
• FlyBaseID: FBgn0264598
• Length: 2777
• Species: Drosophila melanogaster
• Sequence 2: XP_017168158.1
• Gene: Arhgef10 / 234094
• MGIID: 2444453
• Length: 1468
• Species: Mus musculus
• Alignment Length: 1645
• Identity: 311/1645 - (18%)
• Similarity: 514/1645 - (31%)
• Gaps: 541/1645 - (32%)

  Fly   430 FPLNELVHPDSGVSAGSYKLHAQACP-PPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEM 493
            ||:...:..::. :.|.|||.:..|| .|..|......:|.|..::|...:.:|.:.....|||.
Mouse    35 FPVTRSLRGEAS-TLGHYKLVSFLCPLCPRGRKGPLSPDAVQAWRKEARLSMEQGEASPPVPAEH 98

  Fly   494 AAAVTVT---------------------PQKPVA------------------TGSGSGSNSAAMA 519
            .|....:                     .|.|.|                  .|:|:|:..|..|
Mouse    99 EAKCDTSNNEEEGELFDFDSGDEVPEADRQVPSADDRTRGAEAGGADENTCPAGNGTGAEPAPEA 163

  Fly   520 LNDE-VISISIDSMGKED--PLLPQQPQQPMKLSKKALHQRDA---DENLFLRF----------- 567
            ...: |:...::.....|  ||...||..|           ||   :|.:.||.
Mouse   164 EPAKLVVPTKVNPYSVIDITPLQEDQPSSP-----------DANTEEEGVGLRVPSGYSVPVPCG 217

  Fly   568 ------LELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSG--GS 624
                  |.|..||  .::....:|::...:.:|....:...|.|::        ..:|:|.  ||
Mouse   218 YAVPSNLPLLLPA--YSSPVIIRAESVEEEEAAETVGDGQCNSLSS--------EDLPHSSEQGS 272

  Fly   625 SVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVD 689
            ..|.|:.:.......|                 .|...|. |.|...:..:.....| |.:|:.|
Mouse   273 QEGSALARWAADPANT-----------------AWMENPE-EAIYDDVPRENSDSEP-DEMIYDD 318

  Fly   690 KHNVV----TMPE--------------ADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNN 736
            ..|..    :.||              :|      |:|.:.....|.||.....  .|.||.:..
Mouse   319 VENGEEGGNSSPEYGWSSSEFESYEEPSD------SEGKNGIPRSFLRSSHKKQ--LSHDLTRFK 375

  Fly   737 VHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAA 801
            .|...::          ..|..:..|...:...::....::|.|..:      |..||...:|..
Mouse   376 AHCEEKM----------RGLVASTVGAMEIQQAKQRQERKMQKLMKA------AKEGTKDGLEKT 424

  Fly   802 KRRL----------------HLPQVTFSKES--------------IVPVTDN-------RRRFLL 829
            |..:                |  :..|.:|.              :.|:.:.       ||..|.
Mouse   425 KAAVKRGGSFIRTRSLVSQDH--RCYFEEEQNLFIDVDCKHPEAVLTPMPEGLSQQQVVRRYILG 487

  Fly   830 QLISREQNFTAALHFGVDRFVQPLGERKD-LISPNDHRTLFQNIDELL------------RIAE- 880
            .::..|:|:..||...::::.:||.|.:. |:|....|.:|..:.|:|            |::|
Mouse   488 SIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHSMFQIALASRVSEW 552

  Fly   881 DILEQLCSSDQQDQEPQMNFASRVYLS--KTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIA 943
            |::|.:               ..|:::  ..:.:..||.:|.|....|..|| .|:..|...|:.
Mouse   553 DVVETI---------------GDVFVASFSKSMVLDAYSEYVNNFSTAVAVL-KKTCATKPAFLE 601

  Fly   944 F--ITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREI 1006
            |  :::.:.| .|..|...:.||:|.|.:.:.|:|.:..|......:.......:.||:.
Mouse   602 FLKLSQDSSP-DRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELET----- 660

  Fly  1007 TVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAV----------QGRQWIFGGD-----LS 1056
                 |.|.|.|.:     :|.:.|   .:.|....|:          .|.:::...|     :.
Mouse   661 -----LAEKLNERK-----RDADQR---CEIKQIAKAINERYLNKLLSSGNRYLVRSDDVIETVY 712

  Fly  1057 RVEGRSVKPYWTLLF--SDIIVFAKVS-----------RDRVLFITEEPIPIANVV--------- 1099
            ...|..||.....:|  :|:::.|..|           ..|.|.....|:...:|:
Mouse   713 NDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAVMSQRYLLKWSVPLGHVDVIQYNGGSGAG 777

  Fly  1100 DSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAP-DLTRTPKRGARRKSLILRAPSL--ELKAVWQ 1161
            :.|.|....:.| .|.|..|   |:....|..| .|.:..:......:::.:...|  .|:..:|
Mouse   778 EHCRHHAAHSPE-SLAVVAN---AKPHKVYMGPGQLYQDLQNLLHDLNVVGQISQLIGNLRGSYQ 838

  Fly  1162 NL-----------LQRQIFLVNAAL-------------GSTPLSS-PLDSPDVLNTLVP-LSDIG 1200
            ||           |||.|.....|:             |....|. |.....|.|||.| :.:..
Mouse   839 NLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFTAVFNTLTPAIKESW 903

  Fly  1201 LTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHV--- 1262
            :::..|..:.|               ...|..|      ....|...:.:....|.:..||:
Mouse   904 VSSLQMAKLAL---------------EEENHMG------WFCVDDDGNLAKKETHPLLVGHMPVM 947

  Fly  1263 --EQIELLIDEKCRILN---------KTGTPKSSALHLANWMKGQLDKQQQQARLAAIARSQENV 1316
              :|.|..|:  |...|         :..:|.::...|  |: |....|..|..:.:...|...|
Mouse   948 VAKQPEFKIE--CAAYNPEPYLSNESQPASPSTARGFL--WI-GSCSNQMGQVAIVSFQGSNPKV 1007

  Fly  1317 SAEDEQLIFNSDSEQDERI---TYWTRQQLEKRTKELNLAKENGGLSARPNFGGKRLSGVEELSM 1378
            ..     .||.:|    ||   .|...:  |.:.:|....|:....::|.........|.||.|:
Mouse  1008 IE-----CFNVES----RILCMVYIPAE--ESKPQETTETKDPEATASRAPHVPTICLGTEEGSI 1061

  Fly  1379 SATSDIYSTSEAEGVTSVISQSHSTTSDSQITVRSSPIVLDKLAVCRHCHKNCQQSGPGGARPG- 1442
            |    ||.:|  :|...|..| |....|.. ||.|        ..|          .|.|...|
Mouse  1062 S----IYKSS--QGCKKVRLQ-HFYAPDKS-TVMS--------LAC----------SPQGLYAGL 1100

  Fly  1443 GGGGVNNSSSTPVLLCNSLKVQHSQSSPNR--------CCKLSNGIAGATEMANPKNSNRTGTGT 1499
            ..|.|.:.:..|           :.|||..        |..|....:||..:        :|||.
Mouse  1101 VNGSVASYTKAP-----------APSSPGSPWAEFCLICVFLPCLPSGAYAI--------SGTGC 1146

  Fly  1500 GSVTSMSSSTITGE---------LSSKVVASSSRRETGDTESDVAQLITDEISQSQSEATDDSVG 1555
            |:|....|.....:         :.|.::...:.......:..:|.:.|..| ::|.||..|. |
Mouse  1147 GAVKGYGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTI-ENQLEAHQDE-G 1209

  Fly  1556 AMPNSSRSAGEGNPV--ITGSRLR-------------HLDPPV----------SVSSL--PNGHC 1593
            .:.:....||.|..:  .:||.||             ::|.||          ||:||  .:|..
Mouse  1210 MVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLL 1274

  Fly  1594 SAGVGGGSPKPLPPPRRTRVVAQMCQEPPRPKA-----------NQQVKAIVTIT--ETARIMRS 1645
            ..|...|....||.||...:          ||.           |..||.||:.|  :.....|.
Mouse  1275 MVGTSLGVVVALPVPRLQGI----------PKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARD 1329

  Fly  1646 SPTKGSGSGSATGTGSVGGSPAKYAAC-----HCRCTPEDFTHAQL------------------- 1686
            ||..||         .:.....|...|     .|...|:.:|...|
Mouse  1330 SPRSGS---------ELQDEDPKDLLCSEEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGS 1385

  Fly  1687 -----SPDKMEHQT-----CKLLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLCG----- 1736
                 ....:||::     |.||..|..:..:..|...          .|:.:.|..:||
Mouse  1386 LNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSRPQGSR----------RARASSALVVCGGQGHR 1440

  Fly  1737 ----QERIPKEENSTPTIAV 1752
                :.|.|.:|:...::.|
Mouse  1441 RVHRKARQPSQEDLVSSVMV 1460

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PsGEF	NP_996333.4	DUF4799	<99..209	CDD:318308
		C2	286..>381	CDD:425499
		PDZ_canonical	645..716	CDD:483948	14/88 (16%)
		RhoGEF	835..1007	CDD:470622	41/189 (22%)
		PRK12323	<2304..>2415	CDD:481241
Arhgef10	XP_017168158.1	RhoGEF	482..666	CDD:238091	46/210 (22%)
		PH_19	689..838	CDD:465965	25/152 (16%)
		WD40_2	946..1466	CDD:465964	123/607 (20%)