Protein Alignment Raf and braf

DIOPT Version :9

• Sequence 1: NP_001096867.3
• Gene: Raf / 31221
• FlyBaseID: FBgn0003079
• Length: 739
• Species: Drosophila melanogaster
• Sequence 2: XP_031754388.1
• Gene: braf / 779570
• XenbaseID: XB-GENE-1014202
• Length: 963
• Species: Xenopus tropicalis
• Alignment Length: 833
• Identity: 352/833 - (42%)
• Similarity: 457/833 - (54%)
• Gaps: 129/833 - (15%)

  Fly     3 SESSTEGDSDLYDPL--------------------AEELHNVQLVKHVTRENIDALNAKFANLQE 47
            |..||||.|.|...|                    .||:.|::.:..:|:|:|:||..||.....
 Frog    16 SGGSTEGPSLLNGDLLESETGGLGGAGEMCASGGYPEEVWNIKQMIKLTQEHIEALLDKFGGEHN 80

  Fly    48 PPAMYLIEYQELTSKLHELEAKEQELMERL-NSQDQQEDSSLVERFKEQPHYQNQTQILQQQRQL 111
            ||::|:..|:|.||||..|:.:||:|:|.: |..|...                    |..:..|
 Frog    81 PPSIYVEAYEEYTSKLDLLQQREQQLLESMGNGTDSTS--------------------LPSELTL 125

  Fly   112 ARVHHGNDLTDSLGS---QPGSQCGTLTRQP-KILLRAHLPNQQRTSVEVISGVRLCDALMKALK 172
            :.|.....|..|..|   .|........:.| |.::|..|||:|||.|...|||.:.|:|.|||.
 Frog   126 SSVPSNLSLAPSALSALRTPSDPSRINPKSPQKPIVRVFLPNKQRTVVPARSGVTVRDSLKKALM 190

  Fly   173 LRQLTPDMCEVSTTHSG-RHIIPWHTDIGTLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFC 236
            :|.|.|:.|.|.....| :..|.|.|||..:..||:.|.:|:..|:.|   |..:|||||:|.||
 Frog   191 MRGLIPECCAVYRVQDGEKKPIGWDTDISWITGEELHVEVLENVPLTT---HNFVRKTFFTLAFC 252

  Fly   237 EGCRRLLFTGFYCSQCNFRFHQRCANRVPMLCQPFP-MD-SYYQLLLAENPDNGVGFPGRGTAVR 299
            :.||:|||.||.|..|.::|||||:..||::|..:. :| .:.......:|.:....|....|..
 Frog   253 DFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDRLDLLFVSKFFEHHPISQEEAPFEEVAPA 317

  Fly   300 FNMSS-----RSRSRRCSSSGSSSSSKP---PSSSSGNHRQGRPPRISQDDRSNSAPNVCINNIR 356
            ...|:     .:....|..|.|.|.|.|   |.....:||.    :..|.|||:|||||.||.|.
 Frog   318 AESSTPPPPPDATGPLCLPSTSPSKSIPIPQPFRPDEDHRN----QFGQRDRSSSAPNVHINTIE 378

  Fly   357 SVT---------------------SEVQRSLIMQARPPLPHP--------------CTDHSNSTQ 386
            .|.                     :...|....:...||.|.              |...:....
 Frog   379 PVNIDDLIRDQGLQCSEGAPSVQPTRCHRKYHSRDPSPLLHTLPSEIVFDFESDPVCRGSTPGLS 443

  Fly   387 ASPTSTL----------------KHNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPRI 435
            |:|.::|                :..|..:.|:.|....:.....:.|.::|.|...:|.:|.||
 Frog   444 ATPPASLPGSLTNVKALQKSPGQQRERKSSSSSTEDRSRMKTLGRRDSSDDWEIPDGQITVGQRI 508

  Fly   436 GSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIV 500
            |||||||||:..|||.||||.|||..|:|.|||||||||.:|:||||.|||||||..:||.|.:|
 Frog   509 GSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLRLV 573

  Fly   501 TQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIG 565
            ||||||||||.|:|:.||||::..||||.||.||||||||||:|||||||||||||||||:||||
 Frog   574 TQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIG 638

  Fly   566 DFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGH 630
            ||||||.|:||||..|..|.:||||||||||||||:.|||||||||||||||:|||:...|||.:
 Frog   639 DFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDNNPYSFQSDVYAFGIVLYELMTGQLPYSN 703

  Fly   631 ISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPK 695
            |:|:|||:||||||.|.||.|:|||:.|:|:|||..||:|....:||||..:|..:|.:.|:|||
 Frog   704 INNRDQIIFMVGRGYLSPDFSKVRSNCPKAMKRLMADCLKKKRDERPLFPQILASIELLARSLPK 768

  Fly   696 IHRSASEPNLTQSQLQNDEF-LY-LPSPKTPV-------------NFNNFQFF 733
            ||||||||:|.::..|.::| || ..|||||:             |||...|:
 Frog   769 IHRSASEPSLNRAGFQTEDFSLYTCASPKTPIQAGGYEISPRWVNNFNEPLFY 821

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_001096867.3	RBD_RAF	142..212	CDD:340514	30/70 (43%)
		C1_Raf	221..269	CDD:410361	25/47 (53%)
		STKc_Raf	435..687	CDD:270964	179/251 (71%)
braf	XP_031754388.1	RBD_BRAF	158..236	CDD:340654	32/77 (42%)
		C1	234..293	CDD:412127	29/61 (48%)
		STKc_Raf	508..760	CDD:270964	179/251 (71%)
		DERM	815..962	CDD:405404	3/7 (43%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	376	1.000	Domainoid score	I858
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3197
Inparanoid	1	1.050	569	1.000	Inparanoid score	I1051
OMA	1	1.010	-	-		QHG49873
OrthoDB	1	1.010	-	-		D129822at33208
OrthoFinder	1	1.000	-	-		FOG0001587
OrthoInspector	1	1.000	-	-		otm48176
Panther	1	1.100	-	-	LDO	PTHR44329
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X994
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	12.080