Protein Alignment Raf and raf1b

DIOPT Version :10

• Sequence 1: NP_525047.1
• Gene: Raf / 31221
• FlyBaseID: FBgn0003079
• Length: 739
• Species: Drosophila melanogaster
• Sequence 2: NP_001186684.1
• Gene: raf1b / 557109
• ZFINID: ZDB-GENE-090826-2
• Length: 629
• Species: Danio rerio
• Alignment Length: 593
• Identity: 290/593 - (48%)
• Similarity: 374/593 - (63%)
• Gaps: 54/593 - (9%)

  Fly   143 LRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEV---STTHSGRHI-IPWHTDIGTLH 203
            :|..|||:|||.|.|.:|:.|.:.|:||||:|.|.|:.|.|   ...||.:.: :.|.||..:|.
Zfish    56 IRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLV 120

  Fly   204 VEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVPMLC 268
            .||:.|.:||..|:.|   |..:||||..|.||:.|::.|..||.|..|.::||:.|:.:||.:|
Zfish   121 GEELLVEVLDHVPLTT---HNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMC 182

  Fly   269 QPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSKPPSSSSGNHRQG 333
            ..:  .:..||||         ||..|......:...| |||..:|..|.|.        .||..
Zfish   183 VDW--SNIRQLLL---------FPTPGENGALTLPPLS-SRRMQNSRFSFSP--------YHRYS 227

  Fly   334 RP-----------PRISQDDR--SNSAPNVCINNIRSVTSEVQRSLIMQARPPLPHPCTDHSNST 385
            .|           |.:|...:  |.|.|||   ::.|.|..|..:...:|   |.|   |.|.|.
Zfish   228 TPNALNYITPLPSPGVSLGHKVLSTSTPNV---HMVSTTQPVDEAAYQEA---LRH---DESGSP 283

  Fly   386 Q-ASPTSTLK----HNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVYR 445
            | .|||...|    .:|.:|.|::...|.:..|:.:.|...|.|.|.|:|:..|||||||||||:
Zfish   284 QNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYK 348

  Fly   446 AHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSLY 510
            ..|||.||||.|.|..|:|.|||||:||||:|:||||.||:||||.::|..||||||||||||||
Zfish   349 GKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLY 413

  Fly   511 KHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATAKTR 575
            ||:||.||.|::..|:||.||.||||||||||||||||:|||||||||.|:||||||||||.|.|
Zfish   414 KHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKAR 478

  Fly   576 WSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFM 640
            |||..:..||:||||||||||||||:.||||||||||::|||:|||:...|||..|.|:|||:||
Zfish   479 WSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFM 543

  Fly   641 VGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPNL 705
            ||||.|.||:|::....|:|:|||..|||:.|.::||||..:|:.:|.:..:||||:||||||:|
Zfish   544 VGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSL 608

  Fly   706 TQSQLQND 713
            .::....|
Zfish   609 HRATHTED 616

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_525047.1	RBD_RAF	142..212	CDD:340514	30/72 (42%)
		C1_Raf	221..269	CDD:410361	19/47 (40%)
		STKc_Raf	435..687	CDD:270964	173/251 (69%)
raf1b	NP_001186684.1	Ubl1_cv_Nsp3_N-like	53..129	CDD:475130	30/72 (42%)
		C1_A_C-Raf	134..185	CDD:410420	21/53 (40%)
		Protein Kinases, catalytic domain	319..601	CDD:473864	185/281 (66%)