DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Raf and braf

DIOPT Version :9

Sequence 1:	NP_001096867.3	Gene:	Raf / 31221	FlyBaseID:	FBgn0003079	Length:	739	Species:	Drosophila melanogaster
Sequence 2:	NP_001311445.1	Gene:	braf / 403065	ZFINID:	ZDB-GENE-040805-1	Length:	817	Species:	Danio rerio

Alignment Length:	811	Identity:	351/811 - (43%)
Similarity:	464/811 - (57%)	Gaps:	144/811 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 LAEELHNVQLVKHVTRENIDALNAKFANLQEPPAMYLIEYQELTSKLHELEAKEQELMERLNSQD 81
            :.||:.|::.:..:|:|:::||..||.....||::||..|:|.||||..|:.:||:|:|.:.:..
Zfish    53 IPEEIWNIKQMIKLTQEHLEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLEAIGNGT 117

  Fly    82 Q---QEDSSLV----ERFKEQPHYQNQTQILQQQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQP 139
            :   ....:|:    :..:..|...|...:||..            ||:....|        |.|
Zfish   118 EFCSSPTPTLLDVKGQGIQSAPTAPNTLAVLQTP------------TDATRGNP--------RSP 162

  Fly   140 -KILLRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEVSTTHSG-RHIIPWHTDIGTL 202
             |.::|..|||:|||.|....|:.:.|:|.|||.:|.|.|:.|.|.....| :..|.|.|||..|
Zfish   163 QKPIVRVFLPNKQRTVVPARCGMTVRDSLKKALMMRGLIPECCAVYRVQDGEKKPIGWDTDISWL 227

  Fly   203 HVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVPML 267
            ..||:.|.:|:..|:.|   |..:|||||:|.||:.||:|||.||.|..|.::|||||:..||::
Zfish   228 TGEELHVEVLENVPLTT---HNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLM 289

  Fly   268 CQPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSK-----PPSSSS 327
            |..:   ....||||.               :|.:.........||.|::..|:     |||.|:
Zfish   290 CVNY---DQLDLLLAS---------------KFLVHHPITQEEVSSEGTTPISEMCPSLPPSEST 336

  Fly   328 G------------------------NHRQGRPPRISQDDRSNSAPNVCINNIRSVTSEVQRSLIM 368
            |                        :||.    :..|.|||:|||||.||.|..|..:   .||.
Zfish   337 GSLCHPTVSPSKSIPIPQSFRPGEEDHRN----QFGQRDRSSSAPNVHINTIEPVNID---DLIR 394

  Fly   369 -QARP-----PLPHPC---------------TDHSN--------------ST---QASPTSTL-- 393
             |..|     |..||.               :.|.|              ||   .|:|.::|
Zfish   395 DQGLPRSDGAPSQHPARCLRKNRTRTSSPLLSSHPNDIVFDFEPEPVFRGSTTGLSATPPASLPG 459

  Fly   394 --------------KHNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVY 444
                          :..:|.:.|.|.:....|.|  :.|.::|.|...:|.:|.|||||||||||
Zfish   460 SLPNVKVSKSPCQPRERKPSSSSEDRNKMKTLGR--RDSSDDWEIPEGQITLGQRIGSGSFGTVY 522

  Fly   445 RAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSL 509
            :..|||.||||.|||..|:|.|||||||||.:|:||||.|||||||..:||.|||||||||||||
Zfish   523 KGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYTTKPQLAIVTQWCEGSSL 587

  Fly   510 YKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATAKT 574
            |.|:|:.||||::..||||.||.||||||||||:|||||||||||||||||:||||||||||.|:
Zfish   588 YHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKS 652

  Fly   575 RWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILF 639
            ||||..|..|.:||||||||||||:|:.|||||||||||||||:|||::..|||.:|:|:|||:|
Zfish   653 RWSGSHQFEQLSGSILWMAPEVIRLQDKNPYSFQSDVYAFGIVLYELMSGALPYSNINNRDQIIF 717

  Fly   640 MVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPN 704
            |||||.|.||:|:|||:.|:|:|||..||:|...::||||..:|..:|.:.|:|||||||||||:
Zfish   718 MVGRGYLSPDLSKVRSNCPKAMKRLMADCLKKKREERPLFPQILASIELLARSLPKIHRSASEPS 782

  Fly   705 LTQSQLQNDEF-LY-LPSPKTPVNFNNFQFF 733
            |.::..|.::| || ..|||||:....:..|
Zfish   783 LNRAGFQTEDFSLYTCASPKTPIQAGGYGEF 813

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_001096867.3	RBD_RAF	142..212	CDD:340514	29/70 (41%)
		C1_Raf	221..269	CDD:410361	25/47 (53%)
		STKc_Raf	435..687	CDD:270964	180/251 (72%)
braf	NP_001311445.1	Raf_RBD	166..237	CDD:176411	29/70 (41%)
		C1_1	245..290	CDD:278556	25/44 (57%)
		Pkinase_Tyr	507..764	CDD:285015	183/256 (71%)
		STKc_Raf	513..765	CDD:270964	180/251 (72%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	382	1.000	Domainoid score	I789
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3197
Inparanoid	1	1.050	584	1.000	Inparanoid score	I967
OMA	1	1.010	-	-		QHG49873
OrthoDB	1	1.010	-	-		D129822at33208
OrthoFinder	1	1.000	-	-		FOG0001587
OrthoInspector	1	1.000	-	-		otm26079
orthoMCL	1	0.900	-	-		OOG6_100006
Panther	1	1.100	-	-	LDO	PTHR44329
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2599
SonicParanoid	1	1.000	-	-		X994
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	14.010