DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Raf and Ksr2

DIOPT Version :9

Sequence 1:	NP_001096867.3	Gene:	Raf / 31221	FlyBaseID:	FBgn0003079	Length:	739	Species:	Drosophila melanogaster
Sequence 2:	NP_001299843.1	Gene:	Ksr2 / 333050	MGIID:	3610315	Length:	951	Species:	Mus musculus

Alignment Length:	666	Identity:	179/666 - (26%)
Similarity:	281/666 - (42%)	Gaps:	156/666 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly   145 AHLPNQQRTS----VEVISGVRLCDALMKALKLRQLTPDMCEVS-----TTHSGRHIIPWHTDIG 200
            |:.|..::.|    :::.|||..|:    .:..:|.:|.:.|.|     ..|.     |:.....
Mouse   326 ANTPKAKKKSKPLNLKIHSGVGSCE----NIPAQQRSPLLSERSLRSFFVGHG-----PFLPSTP 381

  Fly   201 TLHVEEIF-------VRLLDKFPIR---THIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFR 255
            .:|.|..|       .|...:.|.|   ..|||:...|.:.|.. |..|.:.:..|..|..|..:
Mouse   382 PVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQT-CTVCGKGMLFGLKCKNCKLK 445

  Fly   256 FHQRCANRVPMLCQPFPMDSYYQLLLAENPD---------------NGVGFPGRGTAVRFNM--- 302
            .|.:|....|. |         .||:....|               |.|..|.|.:.:..:.
Mouse   446 CHNKCTKEAPP-C---------HLLIIHRGDPARLVRTESVPCDINNPVRKPARYSDLHISQTLP 500

  Fly   303 ------------------SSRSRSRRCSSSGSSSSSKPPSSS--SGNHRQGRPPR---------- 337
                              ||...|...|:..|.:...|||::  |..|...:.||
Mouse   501 KTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCPRQKKNFNLPAS 565

  Fly   338 --------------ISQDDRSNSAPNVCINNIRSVTSEVQRSLIMQARPPLPHPCTDHSNSTQAS 388
                          :...:....||.|.::.:.|.|       |::..|.|         ..:..
Mouse   566 HYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNT-------ILEGNPLL---------QIEVE 614

  Fly   389 PTSTLK--HNRPRARSADESNKNLLLRDAKSSE----------ENWNILAEEILIGPRIGSGSFG 441
            |||..:  ||.......:....||.|..|:|..          :.|:|..|::.||..||.|.||
Mouse   615 PTSENEESHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFG 679

  Fly   442 TVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMG-CVSKPSLAIVTQWCE 505
            .||...|||.||::.::::..:..||:|||.||...::|||.|::|||| |:|.|.|||:|..|:
Mouse   680 QVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCK 744

  Fly   506 GSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLA 570
            |.:||..|..::....:|....|.:::.:||.|||||.|:|:||||.|:| :::..|.|.||||.
Mouse   745 GRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVF-YDNGKVVITDFGLF 808

  Fly   571 TAKTRWSGEKQANQ-------PTGSILWMAPEVIRM------QELNPYSFQSDVYAFGIVMYELL 622
            :.    ||..||.:       ..|.:..:|||:||.      ::..|:|..|||:|.|.:.|||.
Mouse   809 SI----SGVLQAGRRDDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELH 869

  Fly   623 AECLPYGHISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLE 687
            |...|: .....:.|::.:|.| ::|::||:  ...:.:..:...|..:..::||.|..|::|||
Mouse   870 AREWPF-KTQPAEAIIWQMGTG-MKPNLSQI--GMGKEISDILLFCWAFEQEERPTFTKLMDMLE 930

  Fly   688 NMLRTLPKIHRSASEP 703
            .    |||.:|..|.|
Mouse   931 K----LPKRNRRLSHP 942

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_001096867.3	RBD_RAF	142..212	CDD:340514	17/82 (21%)
		C1_Raf	221..269	CDD:410361	13/47 (28%)
		STKc_Raf	435..687	CDD:270964	96/265 (36%)
Ksr2	NP_001299843.1	KSR1-SAM	24..152	CDD:290277
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	237..298
		C1	414..456	CDD:237996	10/42 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	489..558		13/68 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	614..634		5/19 (26%)
		PK_KSR2	666..935	CDD:271055	102/281 (36%)
		Pkinase_Tyr	667..929	CDD:285015	97/270 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910