Protein Alignment Raf and LIMK1

DIOPT Version :9

• Sequence 1: NP_001096867.3
• Gene: Raf / 31221
• FlyBaseID: FBgn0003079
• Length: 739
• Species: Drosophila melanogaster
• Sequence 2: NP_511139.2
• Gene: LIMK1 / 32207
• FlyBaseID: FBgn0283712
• Length: 1257
• Species: Drosophila melanogaster
• Alignment Length: 828
• Identity: 178/828 - (21%)
• Similarity: 280/828 - (33%)
• Gaps: 276/828 - (33%)

  Fly   107 QQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQPKILLRAH-----LPNQQRTSVEVISGVRLCDA 166
            |||..|....|.   ..||:..|...|  ...|   |.||     ||:.:...|..:.....||.
  Fly     4 QQRLRANGGRGG---TGLGAGSGPVSG--GHSP---LCAHCRGQLLPHPEEPIVMALGQQWHCDC 60

  Fly   167 LMKALKLRQLTPDMCEVSTTHSGRHIIPWHTD-IGTLHV-EEIFVRLLDKF---------PIRTH 220
            ...:         :||       .|:..|:.: .|.|:. |:.:.|..|..         |:...
  Fly    61 FRCS---------VCE-------GHLHNWYFEREGLLYCREDYYGRFGDACQQCMAVITGPVMVA 109

  Fly   221 IKHQIIRKTFFSLVFCEGCRRLLFTG----------FYCSQCNFRFHQRCANRVPMLCQPF---- 271
            .:|:...:.|    .|..|...:..|          .||.||   :.:|.       |||.
  Fly   110 GEHKFHPECF----CCTACGSFIGEGESYALVERSKLYCGQC---YGKRS-------CQPADAKA 160

  Fly   272 -------PMDSYYQLLLAENPD--------NGVGFPGRGTAVR-------FNMSSRSRSRRCSSS 314
                   ||.|   :.|.|.|.        :||........||       |.:...|.:.:|..:
  Fly   161 RITTAGKPMHS---IRLVEIPKDATPGLRVDGVALDDGCPTVRITDLFCNFFLWLTSMAEQCCGA 222

  Fly   315 -------------GS---SSSSKPPSSSS---------GNHR------QGRPPRI----SQDD-- 342
                         |.   ..:..|.|.||         .|.:      :..|.::    ||.|
  Fly   223 PYRIDVNLTNLHIGDRILEVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQ 287

  Fly   343 RSNSAPNVCINNIRSVTS-EVQRSLIMQARPPLPHPCTDHSNSTQASPT---------------- 390
            |:.||..:.:....|.:| ||.|..:.:..............:|.||.|
  Fly   288 RAMSASTLILPLSTSASSVEVGRERLYKTPGEQGTKARKLRQATNASTTIPPAAGATAMTQLKEK 352

  Fly   391 ---STLKHNRPRARSADESNKNLLLRDAKSSEENWNIL--------AEEILIGPRIGSGSFGTVY 444
               |:|.........|.:.:.:..|.|. |..::..::        |.:::||.::|.|.||.|:
  Fly   353 ERCSSLSKLLDEQHQAQQHSAHPQLYDL-SRTQSCRVVQKPQRIFRATDLVIGEKLGEGFFGKVF 416

  Fly   445 RA--HWHGPVAV-KTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSK-PSLAIVTQWCE 505
            :.  ...|.|.| |.|: :....|| :.|..|||:|:...|.::|.|:|.:.| ..|.:||::..
  Fly   417 KVTHRQSGEVMVLKELH-RADEEAQ-RNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVA 479

  Fly   506 GSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLA 570
            |..|.:.:|...........:.:.|.:|.||.|||:.|||||||.|.|..:.||.||.:.|||||
  Fly   480 GGCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLA 544

  Fly   571 ------------------------------TAKTRWSGEKQANQ---PTGSILWMAPEVIRMQEL 602
                                          :...|.|..:|..|   ..|:..|||||:::..:
  Fly   545 RSVDAPRLPSGNMTPGGYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLK- 608

  Fly   603 NPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFMVGRGLLRPDMSQVRSD----------- 656
              |..:.||::|||::.|:                   :||....||.....||
  Fly   609 --YDEKVDVFSFGIMLCEI-------------------IGRVEADPDFMPRNSDFSLNQQEFREK 652

  Fly   657 ----APQALKRLAEDCIKYTPKDRPLFRPLLNMLENM---------------------------- 689
                .|:...::|..|....|..||.|..|...|:.:
  Fly   653 FCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASS 717

  Fly   690 -----------LRTLPKIHRSASEPNLTQSQLQNDEFLYLPS--PKTP 724
                       |..|.::.:||.:..::..:.:.:..:..|.  ||:|
  Fly   718 EDALSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSP 765

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Raf	NP_001096867.3	RBD_RAF	142..212	CDD:340514	16/76 (21%)
		C1_Raf	221..269	CDD:410361	10/57 (18%)
		STKc_Raf	435..687	CDD:270964	84/303 (28%)
LIMK1	NP_511139.2	LIM1_LIMK	33..88	CDD:188750	14/70 (20%)
		LIM2_LIMK	95..148	CDD:188751	10/59 (17%)
		PDZ_signaling	172..271	CDD:238492	16/98 (16%)
		STKc_LIMK	407..693	CDD:271056	85/309 (28%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45445215
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	1	0.930